ID   A0A1V8TMX4_9PEZI        Unreviewed;      1063 AA.
AC   A0A1V8TMX4;
DT   07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT   07-JUN-2017, sequence version 1.
DT   27-MAR-2024, entry version 27.
DE   RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE            EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE   AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN   ORFNames=B0A48_02189 {ECO:0000313|EMBL:OQO12725.1};
OS   Rachicladosporium antarcticum.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Dothideomycetidae; Cladosporiales; Cladosporiaceae; Rachicladosporium.
OX   NCBI_TaxID=1507870 {ECO:0000313|EMBL:OQO12725.1, ECO:0000313|Proteomes:UP000192596};
RN   [1] {ECO:0000313|Proteomes:UP000192596}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCFEE 5527 {ECO:0000313|Proteomes:UP000192596};
RA   Coleine C., Masonjones S., Stajich J.E.;
RT   "Genomes of endolithic fungi from Antarctica.";
RL   Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OQO12725.1}.
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DR   EMBL; NAJO01000004; OQO12725.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1V8TMX4; -.
DR   STRING; 1507870.A0A1V8TMX4; -.
DR   InParanoid; A0A1V8TMX4; -.
DR   OrthoDB; 656at2759; -.
DR   Proteomes; UP000192596; Unassembled WGS sequence.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR   CDD; cd07960; Anticodon_Ia_Ile_BEm; 1.
DR   Gene3D; 1.10.730.20; -; 1.
DR   Gene3D; 3.40.50.620; HUPs; 2.
DR   Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR033708; Anticodon_Ile_BEm.
DR   InterPro; IPR002301; Ile-tRNA-ligase.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   NCBIfam; TIGR00392; ileS; 1.
DR   PANTHER; PTHR42765:SF1; ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR42765; SOLEUCYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   PRINTS; PR00984; TRNASYNTHILE.
DR   SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR   SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR   SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW   ECO:0000256|RuleBase:RU363035};
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW   ECO:0000256|RuleBase:RU363035};
KW   Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW   ECO:0000256|RuleBase:RU363035};
KW   Reference proteome {ECO:0000313|Proteomes:UP000192596}.
FT   DOMAIN          56..738
FT                   /note="Aminoacyl-tRNA synthetase class Ia"
FT                   /evidence="ECO:0000259|Pfam:PF00133"
FT   DOMAIN          783..930
FT                   /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT                   anticodon-binding"
FT                   /evidence="ECO:0000259|Pfam:PF08264"
FT   REGION          691..716
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1063 AA;  116659 MW;  FACB66F3156CB4AA CRC64;
     MLRFFPSRIL RAAANTLAHP DWSKTLHLPR STFPARPKAA ELQTYRQKCA DDLYAWQATA
     RSKTTREADG GKDVDNEFVL HDGPPYANGS VHAGHALNKI LKDIIVRSQL SRGKRVKYQP
     GWDCHGLPIE LKALQQRSTS GKGNAARDAG VGLSALEIRQ TANALASKTI DEQRAEFKIW
     GVMGEWDTPY KTMDLDFEIR QLGVFREMVE KGLIFRDYRP VYWSPSSRTA LAEAELEYDD
     QHKCSAAFVK MPFAQVPSAL SQYEGIDESR LHALIWTTTP WTLPANKAIA HNNDIEYAVI
     ELTIPGAERK PADQLLLAKD RVEHVLSFLP EGTTYKSIIG STMGSELSGG DATCLNVFQN
     SHSRIVPADF VTATTGTGLV HMAPGHGMED YSVCQKYGIG PAFAPIDDAG CFTIEAFPGS
     SETLTGLFAE TKGAAAVLET LAKPSHASTS PSLLASHPFT HKNPIDWRTK KPVIVRATAQ
     WFADVSKIKT RALEAIEAVR FIPESGRNRL KAFIEGRSQW CISRQRAWGV PIPALYHAET
     GEVCMSDESI GHIIDTIKVR GTDAWFFDAE HEAVWLHSSL EPGKWVRGKD TMDVWFDSGT
     TWTSLGPRSD ATKPLADVYV EGSDQHRGWF QSSLLTAVAT QDVNEKPKAP YGTLITHGFI
     LDGEGRKMSK SLGNVVSPVE IVSGALLLQQ QPKSKPGKSG KVKPQAVPQK SKGRSDSLGP
     DLLRLWVASS DYTRDVPISQ QGLLGVQQAL QKLRVTLKFL CGVLADHDSS TTLPTSDDVT
     LADKMMLSAL ASTSREVWHS YQHYRFHEGV REVTNFVNAD LSALYFEVVK DTLYAGVAAD
     RQRVQGVLTI VLAEMLHWLA PIVPHLIEET AEHLPASMKQ GDLAKPLQRV WTKSYVQTAP
     AHNELVATFR AISAATKIAQ ERARAAGKLG SGLACRVEVK SHPSGGSEMG INDRFIQRTR
     SLLDSGELAA MLVVSQAAMV LQISGSTGEN DTVGQASTAD WSFEEPVTVK LSSNSGDATD
     FTVGSVVVLP PQQSKCVRCW QYTAENEELP CERCLRAVNE KKP
//