ID   A0A1V9X320_9ACAR        Unreviewed;       724 AA.
AC   A0A1V9X320;
DT   07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT   07-JUN-2017, sequence version 1.
DT   24-JAN-2024, entry version 20.
DE   RecName: Full=Cysteine--tRNA ligase, cytoplasmic {ECO:0000256|ARBA:ARBA00039362};
DE            EC=6.1.1.16 {ECO:0000256|ARBA:ARBA00012832};
DE   AltName: Full=Cysteinyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00031499};
GN   ORFNames=BIW11_13197 {ECO:0000313|EMBL:OQR67975.1};
OS   Tropilaelaps mercedesae.
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC   Parasitiformes; Mesostigmata; Gamasina; Dermanyssoidea; Laelapidae;
OC   Tropilaelaps.
OX   NCBI_TaxID=418985 {ECO:0000313|EMBL:OQR67975.1, ECO:0000313|Proteomes:UP000192247};
RN   [1] {ECO:0000313|EMBL:OQR67975.1, ECO:0000313|Proteomes:UP000192247}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Wuxi-XJTLU {ECO:0000313|EMBL:OQR67975.1};
RX   PubMed=28327890; DOI=10.1093/gigascience/gix008;
RA   Dong X., Armstrong S.D., Xia D., Makepeace B.L., Darby A.C., Kadowaki T.;
RT   "Draft genome of the honey bee ectoparasitic mite, Tropilaelaps mercedesae,
RT   is shaped by the parasitic life history.";
RL   Gigascience 6:1-17(2017).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|ARBA:ARBA00001947};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OQR67975.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; MNPL01026465; OQR67975.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1V9X320; -.
DR   STRING; 418985.A0A1V9X320; -.
DR   InParanoid; A0A1V9X320; -.
DR   OrthoDB; 2140072at2759; -.
DR   Proteomes; UP000192247; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004817; F:cysteine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006423; P:cysteinyl-tRNA aminoacylation; IEA:InterPro.
DR   CDD; cd00672; CysRS_core; 1.
DR   Gene3D; 3.40.50.620; HUPs; 1.
DR   HAMAP; MF_00041; Cys_tRNA_synth; 1.
DR   InterPro; IPR015803; Cys-tRNA-ligase.
DR   InterPro; IPR024909; Cys-tRNA/MSH_ligase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR032678; tRNA-synt_1_cat_dom.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   NCBIfam; TIGR00435; cysS; 1.
DR   PANTHER; PTHR10890:SF3; CYSTEINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR   PANTHER; PTHR10890; CYSTEINYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF01406; tRNA-synt_1e; 1.
DR   PRINTS; PR00983; TRNASYNTHCYS.
DR   SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR   SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:OQR67975.1};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW   Reference proteome {ECO:0000313|Proteomes:UP000192247};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT   DOMAIN          35..426
FT                   /note="tRNA synthetases class I catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF01406"
FT   COILED          624..656
FT                   /evidence="ECO:0000256|SAM:Coils"
SQ   SEQUENCE   724 AA;  82820 MW;  0A44EF82F75BCB4C CRC64;
     MEEKRKRCQP IWQVPVGHSA SLKLYNSLTR EKEPFVPQNG RRVTWYNCGP TVYDASHMGH
     ARSYISFDIL RRVLQDYFGY DVFFVMNITD IDDKIIKRAR QNHLFEKYRL AGPATTLVED
     GKRAVQRYRE KANKEEDRDK KAMLERLITQ ADVALANPAD EAGLWEALKD PLSDWLDSQK
     GSSVTDHSIF NNLSRFWEKE FFSDMKELNV LPPDVITRVS EYVPEIIKFV ETIITQGLAY
     ESQGSVYFDT IKFDQCENHF YAKLVPEAFG DEKALQEGEG DLSQGGEKRN SSDFALWKAS
     KPGEPEWDSP WGKGRPGWHI ECSVMASELL GESMDIHSGG YDLKFPHHDN EIAQSEAHFN
     NDSWVKYFLH SGHLTISGCK MSKSLKNFIT IKEALRKNTA RQLRLAFLLH QWKDTLDYSD
     NTMEIAMQYE KVFNEFFLSV KDLVRSGGTS ITESLLKWTA NEKQLNTVFQ HSRASVHKAL
     CDSIDTRSAL DALRDSVSAS NVYMKEMKAA KRTLNVPLLR NVAAYVTRIL RVFGAIESTE
     SIGFPTGGSG GGDASAEAIV TPYLTALSNF RDEVRAHARE CKQTAILKVC DLLRDEVMPE
     LGVRLEDHEG QRTVVKLSDR ETLLRERQAK LKAEEKRRLE KEAKKREQEV AAAEKELLRR
     IPPEELFRRE TGKYSAFDET GLPILDAEGK EVSKGLQKKL KKLQQAHEKK YQEYLAEREN
     NSEQ
//