ID A0A1V9ZV58_9STRA Unreviewed; 455 AA.
AC A0A1V9ZV58;
DT 07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT 07-JUN-2017, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE RecName: Full=tRNA(Phe) 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4)]-methyltransferase {ECO:0000256|ARBA:ARBA00012750};
DE EC=2.1.1.282 {ECO:0000256|ARBA:ARBA00012750};
DE AltName: Full=tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase {ECO:0000256|ARBA:ARBA00030554};
GN ORFNames=THRCLA_05665 {ECO:0000313|EMBL:OQS01903.1};
OS Thraustotheca clavata.
OC Eukaryota; Sar; Stramenopiles; Oomycota; Saprolegniales; Saprolegniaceae;
OC Thraustotheca.
OX NCBI_TaxID=74557 {ECO:0000313|EMBL:OQS01903.1, ECO:0000313|Proteomes:UP000243217};
RN [1] {ECO:0000313|EMBL:OQS01903.1, ECO:0000313|Proteomes:UP000243217}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 34112 {ECO:0000313|EMBL:OQS01903.1,
RC ECO:0000313|Proteomes:UP000243217};
RX PubMed=25527045; DOI=10.1093/gbe/evu276;
RA Misner I., Blouin N., Leonard G., Richards T.A., Lane C.E.;
RT "The secreted proteins of Achlya hypogyna and Thraustotheca clavata
RT identify the ancestral oomycete secretome and reveal gene acquisitions by
RT horizontal gene transfer.";
RL Genome Biol. Evol. 7:120-135(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in
CC tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-
CC carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:36635, Rhea:RHEA-COMP:10378, Rhea:RHEA-
CC COMP:10379, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73543, ChEBI:CHEBI:73550; EC=2.1.1.282;
CC Evidence={ECO:0000256|ARBA:ARBA00001168};
CC -!- SIMILARITY: Belongs to the TYW3 family.
CC {ECO:0000256|ARBA:ARBA00008569}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OQS01903.1}.
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DR EMBL; JNBS01001337; OQS01903.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1V9ZV58; -.
DR STRING; 74557.A0A1V9ZV58; -.
DR OrthoDB; 122704at2759; -.
DR Proteomes; UP000243217; Unassembled WGS sequence.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 2.120.10.80; Kelch-type beta propeller; 2.
DR Gene3D; 3.30.1960.10; tRNA wybutosine-synthesizing-like; 1.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR InterPro; IPR003827; tRNA_yW-synthesising.
DR InterPro; IPR036602; tRNA_yW-synthesising-like_sf.
DR PANTHER; PTHR48330; TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3; 1.
DR PANTHER; PTHR48330:SF1; TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3; 1.
DR Pfam; PF01344; Kelch_1; 1.
DR Pfam; PF13418; Kelch_4; 1.
DR Pfam; PF13854; Kelch_5; 1.
DR Pfam; PF02676; TYW3; 1.
DR SUPFAM; SSF117281; Kelch motif; 1.
DR SUPFAM; SSF111278; SSo0622-like; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000243217};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 7..202
FT /note="tRNA wybutosine-synthesizing protein"
FT /evidence="ECO:0000259|Pfam:PF02676"
SQ SEQUENCE 455 AA; 49351 MW; 94A56A846AF261E0 CRC64;
MLAFDASKRE SLQKLQDAID KSPKGSIDAP IVDMIEALNA HPNYVTSSSY SGRIAVFCGV
AQNEATLMHL ITKGGKWLVS SHAPVSYDEL HAKVLADPSV LNGMVIFKHE PFIMHVQCRD
EEAAKVMLQC GLACGFRESG IVLGNKKTMV AIRTTANSME IPIALNGKLM LDENYLQWIL
AIANEKFEAN RLKTQRFMQE LDSTLFSLPR MLLQDVAASQ ADTVVPRAGH SNKNSLCIVG
GQGQTSNGTA RLSDLVIFDR TTKNVIKTTA LSMSPRMHHT AVERQGKLIL FGGRASPAKP
LNDLISIDMT SFNVENITVD GSPPSPRWGH TSILVGTKMI VIGGRNATSV LSDVHILDLS
SKTPSWKYGA CGKPMFAHSA VNVKGKIYCF GGRSNLDALS DISLDTIQVY DSTLDNWSIE
STSGKTIPSA RSNAQAVIVQ DQHGILTAGI QAERQ
//
