ID   A0A1W1Y3P0_9SPHN        Unreviewed;       428 AA.
AC   A0A1W1Y3P0;
DT   07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT   07-JUN-2017, sequence version 1.
DT   27-MAR-2024, entry version 29.
DE   RecName: Full=Citrate synthase {ECO:0000256|PIRNR:PIRNR001369, ECO:0000256|RuleBase:RU003370};
GN   ORFNames=SAMN06272759_101221 {ECO:0000313|EMBL:SMC30840.1};
OS   Novosphingobium sp. B1.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Novosphingobium.
OX   NCBI_TaxID=1938756 {ECO:0000313|EMBL:SMC30840.1, ECO:0000313|Proteomes:UP000192500};
RN   [1] {ECO:0000313|Proteomes:UP000192500}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B1 {ECO:0000313|Proteomes:UP000192500};
RA   Varghese N., Submissions S.;
RL   Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16;
CC         Evidence={ECO:0000256|ARBA:ARBA00000308,
CC         ECO:0000256|RuleBase:RU003370};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2. {ECO:0000256|ARBA:ARBA00004751,
CC       ECO:0000256|RuleBase:RU003370}.
CC   -!- SIMILARITY: Belongs to the citrate synthase family.
CC       {ECO:0000256|ARBA:ARBA00010566, ECO:0000256|PIRNR:PIRNR001369,
CC       ECO:0000256|RuleBase:RU003406}.
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DR   EMBL; FWXL01000001; SMC30840.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1W1Y3P0; -.
DR   STRING; 1938756.SAMN06272759_101221; -.
DR   OrthoDB; 9800864at2; -.
DR   UniPathway; UPA00223; UER00717.
DR   Proteomes; UP000192500; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06114; EcCS_like; 1.
DR   Gene3D; 2.20.28.60; -; 1.
DR   Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR   Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   InterPro; IPR010953; Citrate_synthase_typ-I.
DR   NCBIfam; TIGR01798; cit_synth_I; 1.
DR   PANTHER; PTHR42871; CITRATE SYNTHASE; 1.
DR   PANTHER; PTHR42871:SF1; CITRATE SYNTHASE; 1.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; Citrate synthase; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR001369};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius};
KW   Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532,
KW   ECO:0000256|RuleBase:RU003370}.
FT   TRANSMEM        364..387
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   ACT_SITE        306
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
FT   ACT_SITE        363
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
SQ   SEQUENCE   428 AA;  47506 MW;  69BCE9F2BC1C8EFB CRC64;
     MSDNQASLTA DGKTIEMPVK SGTIGPDVID IRKLYGQTGM FTYDPGFTST ASCDSAITYI
     DGDEGVLLHR GYPIGQLAEH SSFMEVSYLL LNGELPSSQE LADFSRTITR HTMLHEQLAT
     FYRGFRRDAH PMAIMCGVVG ALSAFYHDST DIADPNHRKI SSHRLIAKMP TIAAMAYKYS
     VGQPFVYPDN KLSYTGNFLK MTFGVPAEEY EVIPAVEKAM DRIFILHADH EQNASTSTVR
     LAGSSGANPF ACIAAGIACL WGPAHGGANE AALNMLKEIG TPDKIPHYIE RAKDKNDPFR
     LMGFGHRVYK NYDPRATVMQ STVREVFDAL KVNDPLFETA LRLEEIALND PYFIEKKLFP
     NVDFYSGIIL NAIGFPTTMF TVLFALARTV GWVAQWNEMI SDPGQKIGRP RQLYTGPTQR
     DYVPLSNR
//