ID A0A1W6L945_9BURK Unreviewed; 726 AA.
AC A0A1W6L945;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU003887};
DE EC=5.4.99.- {ECO:0000256|RuleBase:RU003887};
GN ORFNames=A4W93_12865 {ECO:0000313|EMBL:ARN20714.1};
OS Piscinibacter gummiphilus.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Sphaerotilaceae; Piscinibacter.
OX NCBI_TaxID=946333 {ECO:0000313|EMBL:ARN20714.1, ECO:0000313|Proteomes:UP000193427};
RN [1] {ECO:0000313|EMBL:ARN20714.1, ECO:0000313|Proteomes:UP000193427}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NS21 {ECO:0000313|EMBL:ARN20714.1,
RC ECO:0000313|Proteomes:UP000193427};
RA Tabata M., Kasai D., Fukuda M.;
RT "Complete genome sequence of natural rubber-degrading, novel Gram-negative
RT bacterium, Rhizobacter gummiphilus strain NS21.";
RL Submitted (APR-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000256|ARBA:ARBA00008348, ECO:0000256|RuleBase:RU003887}.
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DR EMBL; CP015118; ARN20714.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1W6L945; -.
DR STRING; 946333.A4W93_12865; -.
DR KEGG; rgu:A4W93_12865; -.
DR Proteomes; UP000193427; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02556; PseudoU_synth_RluB; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.70.1560; Alpha-L RNA-binding motif; 1.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR NCBIfam; TIGR00093; pseudouridine synthase; 1.
DR PANTHER; PTHR47683; PSEUDOURIDINE SYNTHASE FAMILY PROTEIN-RELATED; 1.
DR PANTHER; PTHR47683:SF3; RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE B; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|RuleBase:RU003887};
KW Reference proteome {ECO:0000313|Proteomes:UP000193427};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT DOMAIN 247..309
FT /note="RNA-binding S4"
FT /evidence="ECO:0000259|SMART:SM00363"
FT REGION 1..174
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 200..243
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 485..665
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 702..726
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 131..152
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 201..227
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 486..518
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 564..582
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 726 AA; 76466 MW; CE51F5317E0CFCBB CRC64;
MKQPEDTTAT APDAHTEVPA ADAKPVRKRR TTKAAAAAAE GAAPAVAEVT APAPEADAGA
EAAEAKPARK RRAPAKKAAV ADEAAVSEVP AEAAPVEAPA PAPAPAVASA PRVEAQPAAA
EASAPADGEG DASEARAEGE GEGEGEGRSG RNRRRNRKDR NRGEAREGGE PRADVANLVA
PAALEAGEVF AQVLSGDFDV EPEGEAPEAA AEGDDTAAEE VADAAAEGEE EAPGAPKRVL
QPDADAPKLH KVLAQAGIGS RRDMEELILD GQITVNGEPA HIGQRISFGD RIQVKGKPVR
VRIVPPPPRI IAYHKPVGEV VTHDDPEHRP TVFRRLPRLQ QGKWQSVGRL DLNTEGLLLF
TNSGELANQL MHPRFGVERE YAVRVLGTLE PHAKAKLLEG VQIDGQAAAF KSIEDGGGEG
ANHWYRVVIT EGRNREVRKL FDAVGLTVSR LIRIRYGTVV LPRGLKRGVW VDLSDNDVKT
IRRLAGGNDG QQQQQRGEGR GNGQPQQQRG NEQRQGSAQN RNDRNDRNVR GGNNQNNRGD
RNDRGPRGNR GNGPQQNPIP AQVREPRAER DYDDDDLDFD PSKIPNPLEQ TFDKRFVQGK
SRGPVGGAGF GRGGGGFGAG GSAPPRAPQQ KKGGPKEPDP LQTSMGYLGA DAYHGRKGGG
GRGGNRGGVA GVATAAVAVV ATGAVVAVVA AAAETVRKPP VFEDSPAFRH QGTRLHELES
KALPSA
//
