ID   A0A1W6MJU7_9FLAO        Unreviewed;       545 AA.
AC   A0A1W6MJU7;
DT   05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT   05-JUL-2017, sequence version 1.
DT   27-MAR-2024, entry version 19.
DE   SubName: Full=Peptidase M1 {ECO:0000313|EMBL:ARN77870.1};
GN   ORFNames=BST97_07565 {ECO:0000313|EMBL:ARN77870.1};
OS   Nonlabens spongiae.
OC   Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC   Flavobacteriaceae; Nonlabens.
OX   NCBI_TaxID=331648 {ECO:0000313|EMBL:ARN77870.1, ECO:0000313|Proteomes:UP000193431};
RN   [1] {ECO:0000313|EMBL:ARN77870.1, ECO:0000313|Proteomes:UP000193431}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 13191 {ECO:0000313|EMBL:ARN77870.1,
RC   ECO:0000313|Proteomes:UP000193431};
RA   Kumagai Y.;
RT   "Trade-off between light-utilization and light-protection in marine
RT   flavobacteria.";
RL   Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|PIRSR:PIRSR634015-3};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000256|PIRSR:PIRSR634015-3};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP019344; ARN77870.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1W6MJU7; -.
DR   STRING; 331648.BST97_07565; -.
DR   OrthoDB; 100605at2; -.
DR   Proteomes; UP000193431; Chromosome.
DR   GO; GO:0008237; F:metallopeptidase activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd09603; M1_APN_like; 1.
DR   Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR   Gene3D; 2.60.40.1730; tricorn interacting facor f3 domain; 1.
DR   InterPro; IPR045357; Aminopeptidase_N-like_N.
DR   InterPro; IPR042097; Aminopeptidase_N-like_N_sf.
DR   InterPro; IPR014782; Peptidase_M1_dom.
DR   InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR   PANTHER; PTHR11533:SF303; AMINOPEPTIDASE N; 1.
DR   PANTHER; PTHR11533; PROTEASE M1 ZINC METALLOPROTEASE; 1.
DR   Pfam; PF01433; Peptidase_M1; 1.
DR   Pfam; PF17900; Peptidase_M1_N; 1.
DR   SUPFAM; SSF63737; Leukotriene A4 hydrolase N-terminal domain; 1.
DR   SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE   4: Predicted;
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR634015-3};
KW   Reference proteome {ECO:0000313|Proteomes:UP000193431};
KW   Signal {ECO:0000256|SAM:SignalP}; Zinc {ECO:0000256|PIRSR:PIRSR634015-3}.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           22..545
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5010867848"
FT   DOMAIN          50..226
FT                   /note="Aminopeptidase N-like N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF17900"
FT   DOMAIN          269..472
FT                   /note="Peptidase M1 membrane alanine aminopeptidase"
FT                   /evidence="ECO:0000259|Pfam:PF01433"
FT   ACT_SITE        338
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-1"
FT   ACT_SITE        412
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-1"
FT   BINDING         337
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-3"
FT   BINDING         341
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-3"
FT   BINDING         360
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634015-3"
SQ   SEQUENCE   545 AA;  63183 MW;  B3E67C1789233C01 CRC64;
     MKNIKIIVAI ILLMGIQQVS AQLLQDKNQF TRQDTLRGSI TQERAWWDLK YYDLEVEVFP
     DRKFIKGQNT ITYTVNEPYQ LMQVDLQAPM KITKVTQNGK NLLVTDEGNT HFIKLINNQK
     TGKEYEVVVH FEGEPREARN APWDGGFSWK KDKNGKHFIA TSNQGLGASV WWPCKDHMYQ
     EPEGMTITAT VPEDLICVSN GRLIKEKNKG GKTAYTWQVK NPINNYGVNL NIGDYVNFSE
     VYDGEKGDLD MDYWVLKDNL EKAKEHFKDA PKMMKAFEHW FGPYPFYEDS FKLVEVPYLG
     MEHQSSVTYG NKYVKGYLGQ DLSRTGWGLK FDFIIIHEAG HEWFANNITN KDIADMWIHE
     GFTAYSESLF LDYHYGTDAA NAYVIGTRRS IQNDRPLIGV YGVNNEGSGD MYYKGANLLH
     TLRQIVSNDE KWRSILRGMN ETFYHRTVTT QEIEDYLNKN IDLDLKPIFD QYLRTTDIPL
     FKYEIKDGKL YYHYDNVVTG FSMPLQVAIN GTLHMLQPQE EEQVFSLDQL IEKVEVNPNF
     YVGVQ
//