ID A0A1W6ML22_9FLAO Unreviewed; 950 AA.
AC A0A1W6ML22;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=BST97_09900 {ECO:0000313|EMBL:ARN78277.1};
OS Nonlabens spongiae.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Nonlabens.
OX NCBI_TaxID=331648 {ECO:0000313|EMBL:ARN78277.1, ECO:0000313|Proteomes:UP000193431};
RN [1] {ECO:0000313|EMBL:ARN78277.1, ECO:0000313|Proteomes:UP000193431}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 13191 {ECO:0000313|EMBL:ARN78277.1,
RC ECO:0000313|Proteomes:UP000193431};
RA Kumagai Y.;
RT "Trade-off between light-utilization and light-protection in marine
RT flavobacteria.";
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
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DR EMBL; CP019344; ARN78277.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1W6ML22; -.
DR STRING; 331648.BST97_09900; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000193431; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06139; DNA_polA_I_Ecoli_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000193431};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 9..270
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 357..538
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 707..914
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
FT REGION 308..344
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 308..334
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 950 AA; 107011 MW; DF8235E10D6DEFBE CRC64;
MTNDGTDDKR LFLLDAYALI FRGYYALIKN PRINSKGMDT SAIMGFTNSL FDVIRREKPE
YLAVAFDKEG SAERTELYED YKANRDETPE AIRIAIPYIQ KILKAMHIPI VEIAGVEADD
LIGTLSKQAE KEGFTVYMVT PDKDYAQLVS ENIFMYRPAR MGNGIEIWGI PEVQKRFEVE
RPEQVIDYLG MMGDASDNIP GLPGVGDKTA KKFLKQFGSM EGLLDNTDQL KGKLKEKVIA
NAELGRLSKQ LATIKLDCDV QFNAKNYTLD DPDVEQVHEV FDELEFRRAK ETMAKIFAPD
GVPGVNYKEA SSASSSSSSS DSNSDGQFSL FDTPGSGTAA EADATGRKTL ATTDHLYQIV
EEGLGMSLFL KKLMQQKSVC FDTETTSLDP LVAELVGIAF SWEAGKGFYL PFPEERDKAQ
EIVNQLKPFF ESNEIEKIGQ NLKYDIKVLD KYDVEVAAPA FDTMLAHYLI NPDMRHNMDV
LAETYLNYTP QSITELISKK GKNQKSMRDV ELEKQKEYAV EDADITLQLK QYFVKELDSA
GTNKLFTEIE MPLVQVLADM EIEGINLDED YLGTLSRKLT EDIAQLEENI YKEAGEEFNI
GSPKQLGEIL FGKLKLVDKP KKTKTGQYST AEDVLSYLAA NHQIIADVLE YRGLTKLQST
YVDALPNQVN PKTQRIHTNY MQTVAATGRL SSTDPNLQNI PIRTERGRQV RNAFIPRDEN
YTLMAADYSQ IELRIIAALS EEENMIAAFK NGEDIHASTA ARVFNVDIEK VTREQRSNAK
TVNFGIIYGV SAFGLSNQTD LDRSEAKELI DTYYKTYPKL RAYMDDQVAF ARENGYVETV
LGRRRYLKDI NSSNNVVRGA AERNAVNAPI QGSAADIIKI AMINIHEKFK ELNCKSKMLL
QVHDELVFDI HNDELEDMKK LIQDEMQNAY KLSVPLDVEV GVGMNWLEAH
//
