ID A0A1W6WDW4_9FIRM Unreviewed; 646 AA.
AC A0A1W6WDW4;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE RecName: Full=Aconitate hydratase A {ECO:0000256|ARBA:ARBA00019378};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
DE AltName: Full=Iron-responsive protein-like {ECO:0000256|ARBA:ARBA00031977};
DE AltName: Full=RNA-binding protein {ECO:0000256|ARBA:ARBA00031081};
GN ORFNames=B6259_05800 {ECO:0000313|EMBL:ARP50435.1};
OS Ruminococcaceae bacterium CPB6.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
OX NCBI_TaxID=1572656 {ECO:0000313|EMBL:ARP50435.1, ECO:0000313|Proteomes:UP000195433};
RN [1] {ECO:0000313|EMBL:ARP50435.1, ECO:0000313|Proteomes:UP000195433}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CPB6 {ECO:0000313|EMBL:ARP50435.1,
RC ECO:0000313|Proteomes:UP000195433};
RA Tao Y.;
RT "Complete genome sequence of Ruminococcaceae bacterial strain CPB6.";
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
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DR EMBL; CP020705; ARP50435.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1W6WDW4; -.
DR STRING; 1572656.B6259_05800; -.
DR KEGG; rbp:B6259_05800; -.
DR OrthoDB; 9764318at2; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000195433; Chromosome.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd01585; AcnA_Bact; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR006250; Aconitase_put.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01342; acon_putative; 1.
DR PANTHER; PTHR43160; ACONITATE HYDRATASE B; 1.
DR PANTHER; PTHR43160:SF3; ACONITATE HYDRATASE, MITOCHONDRIAL; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000195433}.
FT DOMAIN 7..407
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 510..577
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 646 AA; 68881 MW; B915EE0C70D995EE CRC64;
MGLTIAQKII KSHLVSGNMT VSSDIGLHID QTLTQDATGT MAYLEFEAMG IDRVKTEKSV
AYIDHNTLQT GFENADDHRF IQTTAQKHGI WFSRPGNGIC HQVHLERFGV PGKTLIGSDS
HTPTGGGIGM LAIGAGGLDV AVAMGGGAYH INMPKMLRVN LTGKLPAWVS AKDIILKVLQ
VLTVKGGVGK IVEYAGEGVS TLTVPERATI TNMGAELGAT TSIFPSDEVT REFLAAQGRD
ADWKPLASDP DAVYDEEITI DLSSLEPMAA CPHSPDNVRT VRELAGMKID QVCIGSCTNS
SYLDMMRVAS ILKGKTVANN VSLAIAPGSK QVYNMLAKNG ALGDIIGAGA RVLECACGPC
IGMGQSPNSA GISLRTFNRN FEGRSGTKDG QIYLVSPETA AASALAGVMT DPRTLGAENA
IALPKTFLIN DNMVIAPASP EEAKNVEVLR GPNIKPFPST TALAPSIEAK ALLKVGDNIT
TDHIMPAGAK ILPYRSNIPR LSDFCFTVCD PDFPERARKY KKGIIVGGHN YGQGSSREHA
ALVPLYLGIK AVLVKSFARI HCANLVNAGI VPLVFQNQND YDQIDQMDEL SLPNIRAELE
SGLPVTVKNE TKGFAFHADS PLTDRQRKMI LAGGLLNYTK AKHHSV
//
