ID A0A1W9H4J2_9PROT Unreviewed; 734 AA.
AC A0A1W9H4J2;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN ORFNames=A4S08_01370 {ECO:0000313|EMBL:OQW42348.1};
OS Proteobacteria bacterium SG_bin4.
OC Bacteria; Pseudomonadota.
OX NCBI_TaxID=1827381 {ECO:0000313|EMBL:OQW42348.1, ECO:0000313|Proteomes:UP000192864};
RN [1] {ECO:0000313|EMBL:OQW42348.1, ECO:0000313|Proteomes:UP000192864}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SG_bin4 {ECO:0000313|EMBL:OQW42348.1};
RX PubMed=28390307; DOI=10.1016/j.watres.2017.03.042;
RA Wang Y., Ma L., Mao Y., Jiang X., Xia Y., Yu K., Li B., Zhang T.;
RT "Comammox in drinking water systems.";
RL Water Res. 116:332-341(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OQW42348.1}.
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DR EMBL; LWDH01000015; OQW42348.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1W9H4J2; -.
DR STRING; 1827381.A4S08_01370; -.
DR Proteomes; UP000192864; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 6..287
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 288..564
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 27..34
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 734 AA; 82435 MW; D2217A18B919FF00 CRC64;
MSSLLADLNP QQLEAITLPY QSVLVLAGAG SGKTRVLTTR IAYLIQSGQV SPHGILAVTF
TNKAAKEMLA RISAMLPINL RGMWIGTFHG LCHRMLRTHF QDAGLPQTFQ ILDSADQLAL
IKRILRDLQA DDKKFPPRQV QWFINNAKES GLRAAYVTVE DAFSRRMLEF YQAYEQQCQR
EGAVDFAELL LRSYELLTRH EPLCQHYRAR FRHILIDEFQ DTNQLQYKWL KLLAGAETDH
AAAVFAVGDD DQSIYAFRGA YSGNMKDFER DFGISTVIKL EQNYRSHGYI LDAANALIEH
NNDRLGKNLW TNEGKGEPLR VYNAPNDYDE AVFIVEEVKA LQADGVALSE IALLYRSNAQ
SRVLEHSLFN AGVPYRVYGG MRFFDRQEIK HALAYLRLIA NPDDDSALLR VINFPARGIG
ARSLEQLLDD AKVQGISLWQ AALRKGGGET SILQKPAEAL KGTTSFVQKM LLMRRDCAAL
PLPQIIEYML THSELSAYYA KEREGVERLE NLHELINAAA SFVHEAENDS LAEFLAHASL
EAGEHQAGNG QNALQLMTIH AAKGLEFHSV FLSGLEEGLF PHENSLNEIN GLSEERRLMY
VAMTRARRHL YLSFAQSRLL HGQTRYNIAS RFLDEIPQDF LKWLKPSSPH NFSFTSNTLN
HSPSAPFFSG SPHRSPNNIA GLPWKIGQSV LHDKFGTGVI IDYEGSGSDT RVQVKFAQAG
TKWLLMSLAK LTAL
//
