ID   A0A1W9HVX5_9PROT        Unreviewed;       444 AA.
AC   A0A1W9HVX5;
DT   05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT   05-JUL-2017, sequence version 1.
DT   27-MAR-2024, entry version 17.
DE   RecName: Full=N-acetylmuramoyl-L-alanine amidase {ECO:0000256|ARBA:ARBA00011901};
DE            EC=3.5.1.28 {ECO:0000256|ARBA:ARBA00011901};
GN   ORFNames=A4S15_11275 {ECO:0000313|EMBL:OQW51404.1};
OS   Proteobacteria bacterium SG_bin8.
OC   Bacteria; Pseudomonadota.
OX   NCBI_TaxID=1827387 {ECO:0000313|EMBL:OQW51404.1, ECO:0000313|Proteomes:UP000192872};
RN   [1] {ECO:0000313|EMBL:OQW51404.1, ECO:0000313|Proteomes:UP000192872}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SG_bin8 {ECO:0000313|EMBL:OQW51404.1};
RX   PubMed=28390307; DOI=10.1016/j.watres.2017.03.042;
RA   Wang Y., Ma L., Mao Y., Jiang X., Xia Y., Yu K., Li B., Zhang T.;
RT   "Comammox in drinking water systems.";
RL   Water Res. 116:332-341(2017).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolyzes the link between N-acetylmuramoyl residues and L-
CC         amino acid residues in certain cell-wall glycopeptides.; EC=3.5.1.28;
CC         Evidence={ECO:0000256|ARBA:ARBA00001561};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OQW51404.1}.
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DR   EMBL; LWDL01000019; OQW51404.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1W9HVX5; -.
DR   STRING; 1827387.A4S15_11275; -.
DR   Proteomes; UP000192872; Unassembled WGS sequence.
DR   GO; GO:0008745; F:N-acetylmuramoyl-L-alanine amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009253; P:peptidoglycan catabolic process; IEA:InterPro.
DR   CDD; cd02696; MurNAc-LAA; 1.
DR   Gene3D; 2.60.40.3500; -; 1.
DR   Gene3D; 3.40.630.40; Zn-dependent exopeptidases; 1.
DR   InterPro; IPR002508; MurNAc-LAA_cat.
DR   PANTHER; PTHR30404; N-ACETYLMURAMOYL-L-ALANINE AMIDASE; 1.
DR   PANTHER; PTHR30404:SF0; N-ACETYLMURAMOYL-L-ALANINE AMIDASE AMIC; 1.
DR   Pfam; PF01520; Amidase_3; 1.
DR   SMART; SM00646; Ami_3; 1.
DR   SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE   4: Predicted;
FT   DOMAIN          275..431
FT                   /note="MurNAc-LAA"
FT                   /evidence="ECO:0000259|SMART:SM00646"
SQ   SEQUENCE   444 AA;  47859 MW;  4C352DA0363FC0AA CRC64;
     MESTRSTLAG TALPITASQR REKKCRTASA ARGITAAVFI LLASFSPVSA QTPAADGRLN
     SVPSALPVAS EARLIGDQRR TRFVMDIDRR LDIAVSLLAE PSRVVVDLPE TFFKVSPDSG
     REGRGLVSGF RFGQVASGKS RIVLDASSPV LIDRAFVLDP LDGAPARLVV DLVKTGQDEF
     LRAVRRSAAT KISDVKDRGR AGERGGATRA ERPLVVLDPG HGGVDTGAVG QDTTPEKVIT
     LEMAKALKAA LEASGQVRVI LTREGDSFIS LPDRVRFARE RQAALFLSIH ADSSAKPAQA
     LSGASVYTLS ETASDEEAAA YAERENKADQ IAGVDLPPDT SDVADILFDL MHRETKNFSV
     HFARQLVSEL KQSVRLVKNP HRFAGFRVLR APDVPSALLE LGYLTNKDDT RLLNSQEWRE
     KTALRLTKAI ISFVSVTVGR SQAP
//