ID A0A1X0DA07_9MYCO Unreviewed; 518 AA.
AC A0A1X0DA07;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE RecName: Full=Diacylglycerol O-acyltransferase {ECO:0000256|ARBA:ARBA00013244, ECO:0000256|RuleBase:RU361241};
DE EC=2.3.1.20 {ECO:0000256|ARBA:ARBA00013244, ECO:0000256|RuleBase:RU361241};
GN ORFNames=BST26_14200 {ECO:0000313|EMBL:ORA68590.1};
OS Mycolicibacterium insubricum.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=444597 {ECO:0000313|EMBL:ORA68590.1, ECO:0000313|Proteomes:UP000192801};
RN [1] {ECO:0000313|EMBL:ORA68590.1, ECO:0000313|Proteomes:UP000192801}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 45130 {ECO:0000313|EMBL:ORA68590.1,
RC ECO:0000313|Proteomes:UP000192801};
RA Tortoli E., Trovato A., Cirillo D.M.;
RT "The new phylogeny of genus Mycobacterium.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00001039,
CC ECO:0000256|RuleBase:RU361241};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004771, ECO:0000256|RuleBase:RU361241}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC {ECO:0000256|ARBA:ARBA00009587, ECO:0000256|RuleBase:RU361241}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ORA68590.1}.
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DR EMBL; MVHS01000035; ORA68590.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1X0DA07; -.
DR STRING; 444597.BST26_14200; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000192801; Unassembled WGS sequence.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR NCBIfam; TIGR02946; acyl_WS_DGAT; 1.
DR PANTHER; PTHR31650:SF1; DIACYGLYCEROL O-ACYLTRANSFERASE TGS4-RELATED; 1.
DR PANTHER; PTHR31650; O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR Pfam; PF03007; WS_DGAT_cat; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000256|RuleBase:RU361241};
KW Glycerol metabolism {ECO:0000256|ARBA:ARBA00022798,
KW ECO:0000256|RuleBase:RU361241};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516,
KW ECO:0000256|RuleBase:RU361241};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098,
KW ECO:0000256|RuleBase:RU361241};
KW Reference proteome {ECO:0000313|Proteomes:UP000192801};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU361241}.
FT DOMAIN 3..267
FT /note="O-acyltransferase WSD1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03007"
FT DOMAIN 320..469
FT /note="O-acyltransferase WSD1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06974"
FT REGION 477..518
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 518 AA; 55441 MW; AE4E9A04221413F1 CRC64;
MVALDTSTAH NTIGIVGIYD PSTRGESRGP VTYEEVLKYL EARLHVAESF RERLVEVPFG
LDRPWWIRDG DFDLEYHLRE IALPAPGSWR QLCTQIARIH ARPLDLSRPP WELYLIDGLD
GVDHLPQGAF AVFLRIHHSA IDGVAGAEIL TAIHSQEPGD SAPPIPEGYH WEPDTVPSDL
DLLSRAALHG VARPVDVLRQ VVSVAPQLPA LVSDTRDPSV TSPATLPKAT RFNQVVSPHR
VFGTAYTTLD VLKEIRAAVP NATINDVGVA VVGGAIRRYL LDKDELPDEA MVAMMPISVR
PTATRASGTA TSETSTQTSG NEYSVAPITM ATDEEDPLVR LARIVTSTAH VKESGAHPVR
ALIAMSEEAI GGLMGTVQRA AVRALSRRGR AVAAHTLVSN VPGPQTPMYF CGAQMVDTTG
LGPVLDGMGL NNGIGSYGNR VTFCFTADRK ALPDPEVYEQ CLSGAVEELL DAARAKVQAE
PAKRAPAKKA PAKAAPVKAA AAKQAPAKKT AVKKAPAK
//
