ID A0A1X0DLS0_9MYCO Unreviewed; 1225 AA.
AC A0A1X0DLS0;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969,
GN ECO:0000313|EMBL:BBZ68493.1};
GN ORFNames=BST26_03400 {ECO:0000313|EMBL:ORA73089.1}, MINS_39220
GN {ECO:0000313|EMBL:BBZ68493.1};
OS Mycolicibacterium insubricum.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=444597 {ECO:0000313|EMBL:ORA73089.1, ECO:0000313|Proteomes:UP000192801};
RN [1] {ECO:0000313|EMBL:ORA73089.1, ECO:0000313|Proteomes:UP000192801}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 45130 {ECO:0000313|EMBL:ORA73089.1,
RC ECO:0000313|Proteomes:UP000192801};
RA Tortoli E., Trovato A., Cirillo D.M.;
RT "The new phylogeny of genus Mycobacterium.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:BBZ68493.1, ECO:0000313|Proteomes:UP000466223}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 16366 {ECO:0000313|EMBL:BBZ68493.1,
RC ECO:0000313|Proteomes:UP000466223};
RX PubMed=31287781;
RA Matsumoto Y., Kinjo T., Motooka D., Nabeya D., Jung N., Uechi K., Horii T.,
RA Iida T., Fujita J., Nakamura S.;
RT "Comprehensive subspecies identification of 175 nontuberculous mycobacteria
RT species based on 7547 genomic profiles.";
RL Emerg. Microbes Infect. 8:1043-1053(2019).
RN [3] {ECO:0000313|EMBL:BBZ68493.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=JCM 16366 {ECO:0000313|EMBL:BBZ68493.1};
RA Matsumoto Y., Motooka D., Nakamura S.;
RL Submitted (FEB-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
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DR EMBL; AP022618; BBZ68493.1; -; Genomic_DNA.
DR EMBL; MVHS01000005; ORA73089.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1X0DLS0; -.
DR STRING; 444597.BST26_03400; -.
DR KEGG; mij:MINS_39220; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000192801; Unassembled WGS sequence.
DR Proteomes; UP000466223; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR CDD; cd18810; SF2_C_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000192801}.
FT DOMAIN 658..819
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 837..994
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1225 AA; 130764 MW; 413086B5569D9A54 CRC64;
MTAPGTVHAQ TPIAGLVDLA LAAPTLRDLT ERAADGPATL DLVGPPAARV FVTAALARGG
PLLVVTATGR EAEDLTAELR GVFGDAAALF PSWETLPHER LSPAVDTVGT RLHLLRRLAH
PDDDRLGPPL QVVVTTARSL LQPMAGGDGA AVDAEPVTLA VGAEADFDDV ITRLVELAYT
RVDMVGKRGE FAVRGGILDI FAPTAEHPVR LEFWGDEVSE MRMFAVADQR SIPEIAVEHL
VAMPCRELLV TDAVREQAAQ LAGATPATDN TVVGTVGEML AKVAEGIPVD GMESLAPVLW
TGQPRLLTDH LPAGAPVLIC DPEKVRSRAA DLIKTGQEFL EASWSVAALG GAAPVDIEAL
GGSGFRTLDE VRAGAGEHPW WTLSQLSNET AVQIDARPAP NTRGAQQSID EVFAMLRAHI
ATGGVAAIVA PGSGTAARVV EQLGEADVAA TLLEPGARPQ PGVVGVLKGP LHSGLILPGA
DLVVITETDL TGNRVTEVAG KRLAAKRRNT VDPLALTAGD LVVHDQHGIG KFIEMTERAV
GGARREYLVL EYASAKRGGG TDKLYVPMDS LDQLSRYVGG ATPTLSRLGG SDWANTKTKA
RKAVREIATE LVALYAKRQA APGHAFGPDT PWQAEMEDAF GFTETVDQLT AITEVKSDME
KSVPMDRVIC GDVGYGKTEI AVRAAFKAVQ DGKQVAVLVP TTLLADQHLQ TFTARMTGFP
VTVKGLSRFT DGSESKAVVA GLADGSVDIV IGTHRLLQTG VRWKDLGLVI VDEEQRFGVE
HKEHIKSLRS NVDVLTMSAT PIPRTLEMSL AGIREMSTIL TPPEERYPVL TYVGPHDDKQ
VAAALRRELL RDGQAFYIHN RVRSIDEAAA RIRALVPEAR VVVAHGQMPE EQLERTVEGF
WNREFDILVC TTIVETGLDI SNANTLIVER ADTFGLSQLH QLRGRVGRSR ERGYAYFLYP
PEQPLTETAH DRLATIAQNN DLGAGMSVAL KDLEIRGAGN VLGVEQSGHV AGVGFDLYVR
LVGEAVEAYR AAADGQPITA TETKEVRIDL PIDAQLPPDY IGSDRLRLEG YRRLASAADD
DAVARVVDEL TDRYGPLPEP ARRLVAVARL RLLCRRYGVT EVSATGSAGQ PSTLRLAPLP
LVDSQQMRLA RVYPGANYRP TTATVQVPIP RAGSGIGAPR IRDVALAQAV ADLLLVLDGQ
PQGEFDITDL ATAMPTRTTN DGGTA
//
