ID A0A1X0NV89_9TRYP Unreviewed; 964 AA.
AC A0A1X0NV89;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
GN ORFNames=TM35_000162200 {ECO:0000313|EMBL:ORC88582.1};
OS Trypanosoma theileri.
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=67003 {ECO:0000313|EMBL:ORC88582.1, ECO:0000313|Proteomes:UP000192257};
RN [1] {ECO:0000313|EMBL:ORC88582.1, ECO:0000313|Proteomes:UP000192257}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Edinburgh {ECO:0000313|EMBL:ORC88582.1};
RA Kelly S., Ivens A., Mott A., O'Neill E., Emms D., Macleod O., Voorheis P.,
RA Matthews J., Matthews K., Carrington M.;
RT "An alternative strategy for trypanosome survival in the mammalian
RT bloodstream revealed through genome and transcriptome analysis of the
RT ubiquitous bovine parasite Trypanosoma (Megatrypanum) theileri.";
RL Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665,
CC ECO:0000256|RuleBase:RU363044};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946,
CC ECO:0000256|RuleBase:RU363044};
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- SIMILARITY: Belongs to the helicase family. PIF1 subfamily.
CC {ECO:0000256|ARBA:ARBA00009781}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ORC88582.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; NBCO01000016; ORC88582.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1X0NV89; -.
DR STRING; 67003.A0A1X0NV89; -.
DR VEuPathDB; TriTrypDB:TM35_000162200; -.
DR OrthoDB; 169314at2759; -.
DR Proteomes; UP000192257; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR CDD; cd18037; DEXSc_Pif1_like; 1.
DR CDD; cd18809; SF1_C_RecD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR010285; DNA_helicase_pif1-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR47642; ATP-DEPENDENT DNA HELICASE; 1.
DR Pfam; PF05970; PIF1; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU363044};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU363044};
KW DNA recombination {ECO:0000256|RuleBase:RU363044};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU363044};
KW Helicase {ECO:0000256|RuleBase:RU363044, ECO:0000313|EMBL:ORC88582.1};
KW Hydrolase {ECO:0000256|RuleBase:RU363044};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU363044};
KW Reference proteome {ECO:0000313|Proteomes:UP000192257};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..17
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 18..964
FT /note="ATP-dependent DNA helicase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5012348863"
FT DOMAIN 302..444
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT REGION 867..886
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 927..964
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 941..956
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 964 AA; 106152 MW; B68642F960E005B2 CRC64;
MLRRLLLPVA NVGGMFATSL SGCGGSMGIS HPSTATTIIR STRTTTLVGS TCPLFCSRRL
IGEKQPKTVR NNVKNNTKKY GKKSITTASN LSSEMMTGAK ESLPPPPPPG TPLANLQKGV
TPPVIKKGST DHQIEPKKVV KRNLEKLPPQ TNKIYEPTNS TLVYNALIGR MTSETSPSVL
CLKSIGFGIN EKRQVYVEKP DVLNQLAQKM REGTASLPAN WPISIIRALG VIMRSRNIPD
PADAIQQMIQ VKINNLTKGH YSTIINTVGE TTETSKFQSV MDELESVELN EEQERVINLA
LKGHLLYIGG SAGTGKTVLL RVIHQRMQAE RLRVAMTATT GVAGCHIGGS TFHHSLGVTS
QGDFLRKNQL LDYDVIIIDE VSMLSKKLFE EFDRVLREEA GTPDLPFGGV QIILCGDFLQ
LGVINEASIL SSKLFQNVFV KVRLGVQVRQ ASDKLFAEAL QQMRVGVVPE TLMESVQQLP
PGTMVESAVN LLPTNKEVQI ANDKELERLP GDAIMLTPET GITALRCDST VTVLLRTESG
FNEEEFSRHI RSLLQATLDL PKASLLSLYR VYEDGHAMRV CLPPGESAAW RDAMRERFLE
VAGLINDLEL GAVVTEILPN ADGLHTPESE EYLQKLMSKH PIAQPITFKK GCRVLLRANL
NSRLVNGSIG TVIDFVECSL DNIPGFLRSE RVDNCVERYR IYCMTECGMQ VPLLPVVKFH
SGEIITVPPW EFTVGGGPIT NYYTLSSVAL PLTLAYAFTV HKVQGLTLVG RVHLELSRMW
PCEHLLYVAM SRVRNPEQLS ISSFTPNMVV ANAECVAFDS SLPTVESVGP TDNYPVSSWK
RCNDTIYHLR RRGASLSHLL RGATLKESEK NSMATGRDSG SVQVAGPLKG SLEHSVLVAR
RMRKLIKQTE RVAKMQESRR RSKTIAIAIN DHNSNNNGSS SNDDDDDDDD NNDDSDTTEV
EHIL
//
