ID A0A1X1MU70_9VIBR Unreviewed; 597 AA.
AC A0A1X1MU70;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=Glutathione-regulated potassium-efflux system protein KefB {ECO:0000256|HAMAP-Rule:MF_01412};
DE AltName: Full=K(+)/H(+) antiporter {ECO:0000256|HAMAP-Rule:MF_01412};
GN Name=kefB {ECO:0000256|HAMAP-Rule:MF_01412};
GN ORFNames=ST37_07940 {ECO:0000313|EMBL:ORT50814.1};
OS Vibrio sp. qd031.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=1603038 {ECO:0000313|EMBL:ORT50814.1, ECO:0000313|Proteomes:UP000193432};
RN [1] {ECO:0000313|EMBL:ORT50814.1, ECO:0000313|Proteomes:UP000193432}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=qd031 {ECO:0000313|Proteomes:UP000193432};
RA Xiang T., Song Y., Huang L., Wang B., Wu P.;
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ORT50814.1, ECO:0000313|Proteomes:UP000193432}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=qd031 {ECO:0000313|Proteomes:UP000193432};
RA Chen G.;
RT "Denovo assembling a bacteria genome.";
RL Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Pore-forming subunit of a potassium efflux system that
CC confers protection against electrophiles. Catalyzes K(+)/H(+) antiport.
CC {ECO:0000256|HAMAP-Rule:MF_01412}.
CC -!- SUBUNIT: Interacts with the regulatory subunit KefG.
CC {ECO:0000256|HAMAP-Rule:MF_01412}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|HAMAP-
CC Rule:MF_01412}; Multi-pass membrane protein {ECO:0000256|HAMAP-
CC Rule:MF_01412}. Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-pass
CC membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 2 (CPA2)
CC transporter (TC 2.A.37) family. KefB subfamily. {ECO:0000256|HAMAP-
CC Rule:MF_01412}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ORT50814.1}.
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DR EMBL; JXQD01000022; ORT50814.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1X1MU70; -.
DR STRING; 1603038.ST37_07940; -.
DR OrthoDB; 9781411at2; -.
DR Proteomes; UP000193432; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0015503; F:glutathione-regulated potassium exporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:1902600; P:proton transmembrane transport; IEA:InterPro.
DR Gene3D; 1.20.1530.20; -; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR HAMAP; MF_01412; K_H_efflux_KefB; 1.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR004771; K/H_exchanger.
DR InterPro; IPR020884; K_H_efflux_KefB.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR003148; RCK_N.
DR NCBIfam; TIGR00932; 2a37; 1.
DR PANTHER; PTHR46157:SF11; GLUTATHIONE-REGULATED POTASSIUM-EFFLUX SYSTEM PROTEIN KEFB; 1.
DR PANTHER; PTHR46157; K(+) EFFLUX ANTIPORTER 3, CHLOROPLASTIC; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
DR Pfam; PF02254; TrkA_N; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS51201; RCK_N; 1.
PE 3: Inferred from homology;
KW Antiport {ECO:0000256|ARBA:ARBA00022449, ECO:0000256|HAMAP-Rule:MF_01412};
KW Cell inner membrane {ECO:0000256|HAMAP-Rule:MF_01412};
KW Cell membrane {ECO:0000256|HAMAP-Rule:MF_01412};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065, ECO:0000256|HAMAP-
KW Rule:MF_01412};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|HAMAP-Rule:MF_01412};
KW Potassium {ECO:0000256|ARBA:ARBA00022958, ECO:0000256|HAMAP-Rule:MF_01412};
KW Potassium transport {ECO:0000256|ARBA:ARBA00022538, ECO:0000256|HAMAP-
KW Rule:MF_01412}; Reference proteome {ECO:0000313|Proteomes:UP000193432};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|HAMAP-
KW Rule:MF_01412};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, ECO:0000256|HAMAP-
KW Rule:MF_01412};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|HAMAP-Rule:MF_01412}.
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 33..51
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 57..76
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 88..110
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 148..173
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 179..200
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 236..255
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 267..287
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 293..312
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT TRANSMEM 355..374
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01412"
FT DOMAIN 400..522
FT /note="RCK N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51201"
SQ SEQUENCE 597 AA; 64955 MW; 413336DA7375C1C6 CRC64;
MALGNEFLQN SVTFLVAAVA AVPLAQRLGL GSVLGYLLAG VAIGPWGLGL IKDVESILHF
SEFGVVLLLF LIGLELNPKK LWQMRRPIIG LGGAQVLVTS ALLTGAVSLL GFSWNVSTAI
AMGLALSSTA IALRVIEEQG LAGSETGQSG FAVLLFQDIA VIPMLALLPV LAGNVAGDWL
SALSTIAGVL ALLVAGHFLL RPLFRYAIST RTREMFTVTA LLVVVGIAYV MNFMGLSMAL
GSFLAGVLLA ESEFRHELEI TIEPFKGLLL GLFFMSVGMA VNLGLLALYP LEIFTAVIAL
VVTKALVLYV LARLFGTRAK ARSKMAAILS QGGEFAFVLY TAAQSQGLLD AETVSFLLVI
VSLSMVTTPL LLIAQDRWFA RTINEPEFNE PQTVIDKKPR VIVAGFGRFG QIVGRLMYAN
KVKITILESD ASQIQLLRKY GYKVYYGDAT NLELLRSAGV ETAEAILLCT DSPDEILAIV
GLCKQHFPHL KVMARARSRV EAYQLINHGV DNFSRETFLG ALDLGRQALI ELGMHPYQAA
RAEAHFKKLD NRMLEELLPQ HTDDKDLALR AKEARKELEE IFGREMEKDK PQGHDWS
//
