ID A0A1X1QVK7_MYCFA Unreviewed; 939 AA.
AC A0A1X1QVK7;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595,
GN ECO:0000313|EMBL:BBY97152.1};
GN ORFNames=AWC04_19935 {ECO:0000313|EMBL:ORU95408.1}, MFAL_06190
GN {ECO:0000313|EMBL:BBY97152.1};
OS Mycolicibacterium fallax (Mycobacterium fallax).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=1793 {ECO:0000313|EMBL:ORU95408.1, ECO:0000313|Proteomes:UP000193484};
RN [1] {ECO:0000313|EMBL:ORU95408.1, ECO:0000313|Proteomes:UP000193484}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44179 {ECO:0000313|EMBL:ORU95408.1,
RC ECO:0000313|Proteomes:UP000193484};
RA Tarcisio F., Conor M., Antonella G., Elisabetta G., Giulia F.S., Sara T.,
RA Anna F., Clotilde B., Roberto B., Veronica D.S., Fabio R., Monica P.,
RA Olivier J., Enrico T., Nicola S.;
RT "The new phylogeny of the genus Mycobacterium.";
RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:BBY97152.1, ECO:0000313|Proteomes:UP000467101}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 6405 {ECO:0000313|EMBL:BBY97152.1,
RC ECO:0000313|Proteomes:UP000467101};
RX PubMed=31287781;
RA Matsumoto Y., Kinjo T., Motooka D., Nabeya D., Jung N., Uechi K., Horii T.,
RA Iida T., Fujita J., Nakamura S.;
RT "Comprehensive subspecies identification of 175 nontuberculous mycobacteria
RT species based on 7547 genomic profiles.";
RL Emerg. Microbes Infect. 8:1043-1053(2019).
RN [3] {ECO:0000313|EMBL:BBY97152.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=JCM 6405 {ECO:0000313|EMBL:BBY97152.1};
RA Matsumoto Y., Motooka D., Nakamura S.;
RL Submitted (FEB-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946,
CC ECO:0000256|HAMAP-Rule:MF_00595};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
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DR EMBL; AP022603; BBY97152.1; -; Genomic_DNA.
DR EMBL; LQOJ01000082; ORU95408.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1X1QVK7; -.
DR STRING; 1793.AWC04_19935; -.
DR KEGG; mfx:MFAL_06190; -.
DR OrthoDB; 9768133at2; -.
DR Proteomes; UP000193484; Unassembled WGS sequence.
DR Proteomes; UP000467101; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW Rule:MF_00595};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW Pyruvate {ECO:0000313|EMBL:ORU95408.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000193484}.
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 165
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10111"
FT ACT_SITE 597
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10112"
SQ SEQUENCE 939 AA; 102519 MW; BD2BE3B36ECCF816 CRC64;
MAAPRDADPD TALAPIGDVH RTQSGRDATE PMRADIRLLG GILGDTIREQ NGEAVFDLVE
RARTEAFRVR RSEIDRAEMA ALFDGLDTAG AIPVIRAFTN FALLANVAED IHRERRRAIH
IAAGRPPQDS SLAASYAKLD AADVGAELVG ERLAGALVAP VITAHPTETR RRTVFETQNR
ITELMRARVP GGTRTTDGTD VEPALRRHVL RLWQTALIRL SRLRIIDEIE SGLRYYPAAF
FEVIPAVNTE VRAALRARWP GTDLLAEPIL RPGSWIGGDR DGNPNVTAEV VRQATGRAAC
TAFEHYLAEL TVLEIELPLS ARLVTVSAEL DALAGQVDQP LRADEPYRRA VRVIHGRLTA
TAAAILDERP ANLRELGLPA YHRAGELLAD LDIVDDSLRG HGDALLADDR LARLREAVRT
FGFQLCGLDM RQNSESHEQV VAELLAYAGV HPDYRGLDED RRVALLTAEL STRRPLTRPG
DGLSELARKE LGVLAAAAEA VRRYGPDTVP NYIISMCQSV SDLLEAALLL KEAGLLDASG
ERPHAPVGIV PLFETIEDLQ AGASIMAAAL DLPLYRRIVA ARGDRQEVML GYSDSNKDGG
YLAANWALYR AELDLVEMAQ HTGIRLRLFH GRGGTVGRGG GPSYDAILAQ PPGAVRGSLR
LTEQGEVIAA KYAEPKMARR NLETLVAATL ESTLLDVEGL GAGAEAAYAV LDDLAARARD
CYADLVHRTP GFVEYFQTST PVGEIGALNI GSRPTARKQT TAVSDLRAIP WVLAWSQSRV
MLPGWYGTGT AFADYLAEGT ARGEDRLAVL AELYRRWPFF TTVLSNMAQV LAKSDMGLAA
RYAELVEDPA LRERVFGKIV AEHDRCIAMY QAITGHDDLL ADNPALARSV FNRFPYLEPL
NHLQVELLRR YRRGDDDELV QRGILMTMSG LATALRNSG
//
