ID A0A1X1STK6_9MYCO Unreviewed; 329 AA.
AC A0A1X1STK6;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=cholesterol 7-desaturase {ECO:0000256|ARBA:ARBA00026095};
DE EC=1.14.19.21 {ECO:0000256|ARBA:ARBA00026095};
GN ORFNames=AWC00_26695 {ECO:0000313|EMBL:ORV34072.1}, MCNS_16290
GN {ECO:0000313|EMBL:BBZ38566.1};
OS Mycobacterium conspicuum.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycobacterium.
OX NCBI_TaxID=44010 {ECO:0000313|EMBL:BBZ38566.1, ECO:0000313|Proteomes:UP000467385};
RN [1] {ECO:0000313|EMBL:ORV34072.1, ECO:0000313|Proteomes:UP000193407}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44136 {ECO:0000313|EMBL:ORV34072.1,
RC ECO:0000313|Proteomes:UP000193407};
RA Tarcisio F., Conor M., Antonella G., Elisabetta G., Giulia F.S., Sara T.,
RA Anna F., Clotilde B., Roberto B., Veronica D.S., Fabio R., Monica P.,
RA Olivier J., Enrico T., Nicola S.;
RT "The new phylogeny of the genus Mycobacterium.";
RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:BBZ38566.1, ECO:0000313|Proteomes:UP000467385}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 14738 {ECO:0000313|EMBL:BBZ38566.1,
RC ECO:0000313|Proteomes:UP000467385};
RX PubMed=31287781;
RA Matsumoto Y., Kinjo T., Motooka D., Nabeya D., Jung N., Uechi K., Horii T.,
RA Iida T., Fujita J., Nakamura S.;
RT "Comprehensive subspecies identification of 175 nontuberculous mycobacteria
RT species based on 7547 genomic profiles.";
RL Emerg. Microbes Infect. 8:1043-1053(2019).
RN [3] {ECO:0000313|EMBL:BBZ38566.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=JCM 14738 {ECO:0000313|EMBL:BBZ38566.1};
RA Matsumoto Y., Motooka D., Nakamura S.;
RL Submitted (FEB-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cholesterol + H(+) + NADH + O2 = 7-dehydrocholesterol + 2 H2O
CC + NAD(+); Xref=Rhea:RHEA:51644, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:16113, ChEBI:CHEBI:17759,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.14.19.21;
CC Evidence={ECO:0000256|ARBA:ARBA00029355};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:51645;
CC Evidence={ECO:0000256|ARBA:ARBA00029355};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cholesterol + H(+) + NADPH + O2 = 7-dehydrocholesterol + 2 H2O
CC + NADP(+); Xref=Rhea:RHEA:45024, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16113,
CC ChEBI:CHEBI:17759, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC EC=1.14.19.21; Evidence={ECO:0000256|ARBA:ARBA00029369};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45025;
CC Evidence={ECO:0000256|ARBA:ARBA00029369};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|ARBA:ARBA00001962};
CC -!- PATHWAY: Hormone biosynthesis. {ECO:0000256|ARBA:ARBA00004972}.
CC -!- PATHWAY: Steroid hormone biosynthesis; dafachronic acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00025712}.
CC -!- SIMILARITY: Belongs to the cholesterol 7-desaturase family.
CC {ECO:0000256|ARBA:ARBA00025729}.
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DR EMBL; AP022613; BBZ38566.1; -; Genomic_DNA.
DR EMBL; LQOR01000080; ORV34072.1; -; Genomic_DNA.
DR STRING; 44010.AWC00_26695; -.
DR OrthoDB; 5243643at2; -.
DR Proteomes; UP000193407; Unassembled WGS sequence.
DR Proteomes; UP000467385; Chromosome.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProt.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:UniProt.
DR GO; GO:0008203; P:cholesterol metabolic process; IEA:InterPro.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR CDD; cd03469; Rieske_RO_Alpha_N; 1.
DR Gene3D; 2.102.10.10; Rieske [2Fe-2S] iron-sulphur domain; 1.
DR InterPro; IPR045605; KshA-like_C.
DR InterPro; IPR017941; Rieske_2Fe-2S.
DR InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR PANTHER; PTHR21266:SF60; CHOLESTEROL 7-DESATURASE; 1.
DR PANTHER; PTHR21266; IRON-SULFUR DOMAIN CONTAINING PROTEIN; 1.
DR Pfam; PF19298; KshA_C; 1.
DR Pfam; PF00355; Rieske; 1.
DR SUPFAM; SSF55961; Bet v1-like; 1.
DR SUPFAM; SSF50022; ISP domain; 1.
DR PROSITE; PS51296; RIESKE; 1.
PE 3: Inferred from homology;
KW 2Fe-2S {ECO:0000256|ARBA:ARBA00022714};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 13..116
FT /note="Rieske"
FT /evidence="ECO:0000259|PROSITE:PS51296"
SQ SEQUENCE 329 AA; 37512 MW; 73FCFD08168F6A05 CRC64;
MAKPPLSMKP TGWFQVAWSD EIGAGDVHTM KYFDQEMVAW RAESGALTVM NAYCEHLGAH
LGFGGKVVGE VLQCPFHGWQ WSQQGRNVCI PYQDRPNRGR RIRTYPVVER NESIYIWHDV
EGREPYFDAP DIFAGFNDGS SADDYYPQQR LFRAGLELHP QYVLENGVDF AHFKYVHATP
IVPVFTRHDF AEPVSFVDFT ITFEGDDNQK IEDIDSRVEA INGGLGIAVT KSYGMVDNRT
ISAITPVDDS TSDVRFMVYI GRKPSKDPQR AEAKAREFGQ EVIRQFEQDI HIWSHQRYSD
PPALAPDEYE GFTAIRNWAK QFYAAPQKG
//
