ID A0A1X1T6M7_9MYCO Unreviewed; 165 AA.
AC A0A1X1T6M7;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=3'-5' exoribonuclease {ECO:0000256|HAMAP-Rule:MF_00977};
DE EC=3.1.13.- {ECO:0000256|HAMAP-Rule:MF_00977};
GN ORFNames=AWC01_11875 {ECO:0000313|EMBL:ORV40241.1}, MDOR_23890
GN {ECO:0000313|EMBL:BBZ08220.1};
OS Mycolicibacterium doricum.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=126673 {ECO:0000313|EMBL:ORV40241.1, ECO:0000313|Proteomes:UP000193564};
RN [1] {ECO:0000313|EMBL:ORV40241.1, ECO:0000313|Proteomes:UP000193564}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44339 {ECO:0000313|EMBL:ORV40241.1,
RC ECO:0000313|Proteomes:UP000193564};
RA Tarcisio F., Conor M., Antonella G., Elisabetta G., Giulia F.S., Sara T.,
RA Anna F., Clotilde B., Roberto B., Veronica D.S., Fabio R., Monica P.,
RA Olivier J., Enrico T., Nicola S.;
RT "The new phylogeny of the genus Mycobacterium.";
RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:BBZ08220.1, ECO:0000313|Proteomes:UP000467201}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 12405 {ECO:0000313|EMBL:BBZ08220.1,
RC ECO:0000313|Proteomes:UP000467201};
RX PubMed=31287781;
RA Matsumoto Y., Kinjo T., Motooka D., Nabeya D., Jung N., Uechi K., Horii T.,
RA Iida T., Fujita J., Nakamura S.;
RT "Comprehensive subspecies identification of 175 nontuberculous mycobacteria
RT species based on 7547 genomic profiles.";
RL Emerg. Microbes Infect. 8:1043-1053(2019).
RN [3] {ECO:0000313|EMBL:BBZ08220.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=JCM 12405 {ECO:0000313|EMBL:BBZ08220.1};
RA Matsumoto Y., Motooka D., Nakamura S.;
RL Submitted (FEB-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Exonuclease that cleaves single-stranded 3' overhangs of
CC double-stranded RNA. {ECO:0000256|HAMAP-Rule:MF_00977}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00977};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|HAMAP-Rule:MF_00977};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00977}.
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DR EMBL; AP022605; BBZ08220.1; -; Genomic_DNA.
DR EMBL; LQOS01000030; ORV40241.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1X1T6M7; -.
DR STRING; 126673.AWC01_11875; -.
DR KEGG; mdr:MDOR_23890; -.
DR OrthoDB; 4640719at2; -.
DR Proteomes; UP000193564; Unassembled WGS sequence.
DR Proteomes; UP000467201; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0004532; F:RNA exonuclease activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR HAMAP; MF_00977; 3_5_Exoribonuc_actinobact; 1.
DR InterPro; IPR030853; 3_5_Exoribonuc_actinobac.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR033390; Rv2179c-like.
DR NCBIfam; NF033638; RNase_AS; 1.
DR Pfam; PF16473; Rv2179c-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 3: Inferred from homology;
KW Exonuclease {ECO:0000256|HAMAP-Rule:MF_00977};
KW Hydrolase {ECO:0000256|HAMAP-Rule:MF_00977};
KW Magnesium {ECO:0000256|HAMAP-Rule:MF_00977};
KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_00977};
KW Nuclease {ECO:0000256|HAMAP-Rule:MF_00977};
KW Reference proteome {ECO:0000313|Proteomes:UP000193564}.
FT DOMAIN 2..150
FT /note="3'-5' exoribonuclease Rv2179c-like"
FT /evidence="ECO:0000259|Pfam:PF16473"
FT REGION 6..9
FT /note="RNA binding"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00977"
FT BINDING 6
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00977"
SQ SEQUENCE 165 AA; 19022 MW; 16CF654E5746C1D5 CRC64;
MRYFYDTEFI DNGRTIELIS IGVAAEDGRE YYAVSTEFDP ERAGSWVRKH VLPKLPSPAS
KSWRSRREIR SELEDFFGID GEDPIELWAW VGAYDHVVLC QLWGPMTALP PAIPRFTREL
RQFWEDRGSP RMPPRPRDSH DALVDARHNL LRYQVMTGTA GMAGP
//
