ID   A0A1X1T8M7_9MYCO        Unreviewed;       448 AA.
AC   A0A1X1T8M7;
DT   05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT   05-JUL-2017, sequence version 1.
DT   27-MAR-2024, entry version 24.
DE   SubName: Full=Type VII secretion-associated serine protease mycosin {ECO:0000313|EMBL:ORV40906.1};
GN   ORFNames=AWC01_10350 {ECO:0000313|EMBL:ORV40906.1};
OS   Mycolicibacterium doricum.
OC   Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=126673 {ECO:0000313|EMBL:ORV40906.1, ECO:0000313|Proteomes:UP000193564};
RN   [1] {ECO:0000313|EMBL:ORV40906.1, ECO:0000313|Proteomes:UP000193564}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 44339 {ECO:0000313|EMBL:ORV40906.1,
RC   ECO:0000313|Proteomes:UP000193564};
RA   Tarcisio F., Conor M., Antonella G., Elisabetta G., Giulia F.S., Sara T.,
RA   Anna F., Clotilde B., Roberto B., Veronica D.S., Fabio R., Monica P.,
RA   Olivier J., Enrico T., Nicola S.;
RT   "The new phylogeny of the genus Mycobacterium.";
RL   Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004162};
CC       Single-pass membrane protein {ECO:0000256|ARBA:ARBA00004162}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004167}; Single-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004167}.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family.
CC       {ECO:0000256|ARBA:ARBA00011073, ECO:0000256|PROSITE-ProRule:PRU01240}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:ORV40906.1}.
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DR   EMBL; LQOS01000028; ORV40906.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1X1T8M7; -.
DR   STRING; 126673.AWC01_10350; -.
DR   OrthoDB; 9798386at2; -.
DR   Proteomes; UP000193564; Unassembled WGS sequence.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.200; Peptidase S8/S53 domain; 1.
DR   InterPro; IPR000209; Peptidase_S8/S53_dom.
DR   InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR   InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR   InterPro; IPR023834; T7SS_pept_S8A_mycosin.
DR   NCBIfam; TIGR03921; T7SS_mycosin; 1.
DR   PANTHER; PTHR43806:SF59; CEREVISIN-RELATED; 1.
DR   PANTHER; PTHR43806; PEPTIDASE S8; 1.
DR   Pfam; PF00082; Peptidase_S8; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   SUPFAM; SSF52743; Subtilisin-like; 1.
DR   PROSITE; PS51892; SUBTILASE; 1.
PE   3: Inferred from homology;
KW   Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU01240};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW   ProRule:PRU01240}; Reference proteome {ECO:0000313|Proteomes:UP000193564};
KW   Serine protease {ECO:0000256|ARBA:ARBA00022825, ECO:0000256|PROSITE-
KW   ProRule:PRU01240}; Signal {ECO:0000256|SAM:SignalP};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           24..448
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5012077921"
FT   TRANSMEM        421..440
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          83..381
FT                   /note="Peptidase S8/S53"
FT                   /evidence="ECO:0000259|Pfam:PF00082"
FT   REGION          26..48
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          160..180
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        92
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        123
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        334
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01240"
SQ   SEQUENCE   448 AA;  46034 MW;  A416CE70CD162CC7 CRC64;
     MQRVGVVLAA LLLALASAPP VGAIEPPSID PAALPPDQTG PDSPTEQRRI CSAPTVLPNF
     NFADKPWPND YLQLAEAQEF ATGAGVTVAV IDTGVNGSTR VPAEPGGDFV DQAGNGMSDC
     DSHGTLTASI IAGRRSPTDG FVGVAPDARV LSLRQTSEAF QPVGARQDPN DPNTTPTAGS
     LRSLSRAVVH AANLGAQVIN ISEAACYRIT RPIDETGLGA AINYAVNVRN AVVVVAAGNT
     GQDCTQNPPP DPAIPADPRG WQQVQTVVSP AWYSPLVLTV GSINDNGQPS NFSMSGPWVG
     AAAPGANITA LGYDGKPVNA LQGQDGPVPI GGTSFSAAYV SGLAALIKQR FPALTPAQIM
     NRITATARHP GGGVDNYVGA GVIDPVAALT WEVPEGPAAI PYRVKQLPAP VYVPPPDRGP
     ITGVVVIGAG LTLILGITAL TRRALRRR
//