ID A0A1X2GQK2_9FUNG Unreviewed; 906 AA.
AC A0A1X2GQK2;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=sn-1-specific diacylglycerol lipase {ECO:0000256|ARBA:ARBA00026104};
DE EC=3.1.1.116 {ECO:0000256|ARBA:ARBA00026104};
GN ORFNames=DM01DRAFT_1211570 {ECO:0000313|EMBL:ORX59026.1};
OS Hesseltinella vesiculosa.
OC Eukaryota; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina;
OC Mucoromycetes; Mucorales; Cunninghamellaceae; Hesseltinella.
OX NCBI_TaxID=101127 {ECO:0000313|EMBL:ORX59026.1, ECO:0000313|Proteomes:UP000242146};
RN [1] {ECO:0000313|EMBL:ORX59026.1, ECO:0000313|Proteomes:UP000242146}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL 3301 {ECO:0000313|EMBL:ORX59026.1,
RC ECO:0000313|Proteomes:UP000242146};
RG DOE Joint Genome Institute;
RA Mondo S.J., Dannebaum R.O., Kuo R.C., Labutti K., Haridas S., Kuo A.,
RA Salamov A., Ahrendt S.R., Lipzen A., Sullivan W., Andreopoulos W.B.,
RA Clum A., Lindquist E., Daum C., Ramamoorthy G.K., Gryganskyi A., Culley D.,
RA Magnuson J.K., James T.Y., O'Malley M.A., Stajich J.E., Spatafora J.W.,
RA Visel A., Grigoriev I.V.;
RT "Pervasive Adenine N6-methylation of Active Genes in Fungi.";
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + H2O = a 2-acylglycerol + a fatty
CC acid + H(+); Xref=Rhea:RHEA:33275, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17389, ChEBI:CHEBI:17815,
CC ChEBI:CHEBI:28868; EC=3.1.1.116;
CC Evidence={ECO:0000256|ARBA:ARBA00024531};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33276;
CC Evidence={ECO:0000256|ARBA:ARBA00024531};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ORX59026.1}.
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DR EMBL; MCGT01000006; ORX59026.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1X2GQK2; -.
DR OrthoDB; 1341793at2759; -.
DR Proteomes; UP000242146; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR CDD; cd00519; Lipase_3; 1.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002921; Fungal_lipase-like.
DR PANTHER; PTHR45792; DIACYLGLYCEROL LIPASE HOMOLOG-RELATED; 1.
DR PANTHER; PTHR45792:SF8; DIACYLGLYCEROL LIPASE-ALPHA; 1.
DR Pfam; PF01764; Lipase_3; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Reference proteome {ECO:0000313|Proteomes:UP000242146};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
FT DOMAIN 581..722
FT /note="Fungal lipase-like"
FT /evidence="ECO:0000259|Pfam:PF01764"
FT REGION 177..205
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 246..297
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 330..353
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 792..825
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 872..906
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 181..205
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 246..261
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 270..286
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 906 AA; 101799 MW; 44359DB29A906D89 CRC64;
MRVRLGSQQY YSSRSHDPHG NWNEGFLFTI SYHNQLFDTI ELDLYDRKKG WRSKTKHIGK
AKLKIAKLKH HEDVFLTFVP MYEYHTKRYL PAHIQLPHNF LKANMPNFPS LPHPMSKKNG
TANPVQEHYL HYEQHPSHRK HSMTPFIGSI QIRIRYQYQH PDDSLDHSTK PVYLPYNASK
EVDPSSPSRP VSLHPHSSQA TSLSSHTMLT MATTQATDDD DLSSSDNDSM LDNASVCCSL
YSTASALDSQ PPRSTPPTKQ VQADEPPTPV PDVVVSPTSP THPPDLEPEV KPSKPDRAWP
FSNTRLRFAA AQSLSASSSA STHRIINRAS STSMSSRTSS VRSRASDCSS HSAMSPSAGF
GFSRCDDDRR HALLSRHGLG NNHHDGASLF SDRDSAISDS ASIRSNNFGD KNFAFRWINE
NFEEVALSHP SLDRMIGMVV SPQTRILVRA VVKMMNAFGQ GFKITSVQLF SSLSMLQKFY
NEIPRSPPPA SKVTDLRFLD EAHYFLGHAI IAYGWRGISY LGDYAKYLKD VMKTRSNKLA
IVRFLKMPPE DLLGYEYGLR KGAVFQPSYF VSVDRGHEAI VLGIRGTWSL YDVITDLVCD
YKPWKGGLVH SGMLASAQWF FTRIVPQIFH YIHQQCKQDK PPHIKSFIIT GHSLGAGTAA
ILTMMIADHM DELLQISGNP NFRLHCYSYA PVASVSLDIS EKYKDYVDSF VCHDDLVARL
SYGTASCTKE LVMDALIAAD GLGGITKVNH DQETRRQCFD IISTRRQEIF QANEPRYPLL
YVPGRVFQFR RRPIPARPPP QRRASVHEPA HPLSSHGQAH RKPSDASVDF TLHESSALLS
EEMLITKTCL EDHMLVTYLK AFQTARENCM RDSHLEKSSA PSSSPTPDQP PPSILLHDPD
GQLSSI
//
