ID A0A1X2I7A9_9FUNG Unreviewed; 1037 AA.
AC A0A1X2I7A9;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=BCR42DRAFT_422184 {ECO:0000313|EMBL:ORZ10848.1};
OS Absidia repens.
OC Eukaryota; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina;
OC Mucoromycetes; Mucorales; Cunninghamellaceae; Absidia.
OX NCBI_TaxID=90262 {ECO:0000313|EMBL:ORZ10848.1, ECO:0000313|Proteomes:UP000193560};
RN [1] {ECO:0000313|EMBL:ORZ10848.1, ECO:0000313|Proteomes:UP000193560}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL 1336 {ECO:0000313|EMBL:ORZ10848.1,
RC ECO:0000313|Proteomes:UP000193560};
RG DOE Joint Genome Institute;
RA Mondo S.J., Dannebaum R.O., Kuo R.C., Labutti K., Haridas S., Kuo A.,
RA Salamov A., Ahrendt S.R., Lipzen A., Sullivan W., Andreopoulos W.B.,
RA Clum A., Lindquist E., Daum C., Ramamoorthy G.K., Gryganskyi A., Culley D.,
RA Magnuson J.K., James T.Y., O'Malley M.A., Stajich J.E., Spatafora J.W.,
RA Visel A., Grigoriev I.V.;
RT "Pervasive Adenine N6-methylation of Active Genes in Fungi.";
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ORZ10848.1}.
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DR EMBL; MCGE01000023; ORZ10848.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1X2I7A9; -.
DR STRING; 90262.A0A1X2I7A9; -.
DR OrthoDB; 656at2759; -.
DR Proteomes; UP000193560; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07960; Anticodon_Ia_Ile_BEm; 1.
DR Gene3D; 1.10.730.20; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 1.10.10.830; Ile-tRNA synthetase CP2 domain-like; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR HAMAP; MF_02002; Ile_tRNA_synth_type1; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033708; Anticodon_Ile_BEm.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023585; Ile-tRNA-ligase_type1.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42765:SF1; ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR42765; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000313|EMBL:ORZ10848.1}; ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000193560}.
FT DOMAIN 113..737
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 781..942
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1037 AA; 118884 MW; 142F0B624937EB65 CRC64;
MSYQWTRPLV GDSLFKHLLQ QRNQQTSKLS SLSLSPLRST RIKQRMYQAM EYATSNTSKS
LQSAPTMKAN KKNPYSDTIL LPKTDFSLRA DAVNREHLFR DRCTKDLYPW QLENNPKDLF
VLHDGPPYAN GSVHSGHAMN KILKDMINRY QVLRGRKVWY RPGWDCHGLP IEMKALEQLR
KKNLHDSLSP MEIRDLARKK ALKEIDIQRT AFKSWGVMGD WNNPYRTLDK DYELRQLDVF
HEMVKRGYIY RQLKPVYWSP SSKSALAESE LEYNEAHKSR SLHVRFQLAT FGTSLQEKWK
KHINNTYAVI WTTTPWTLPS NKAVSVHPKL EYSLVRLADD FSSNGDQAKL YIIASDRVNA
FETELGQAAQ VVDTASGSSL IGSSYKHPLW KGSNMPFIEG HHVTADSGTG LVHTAPGHGM
EDYDACSKYG IAPFSPVGDD GLYTNEVASL GLEGKSAFED GTTAVIELLR NESSIMLEQD
YIHKYPYDWR TKQPVMLRAT AQWFANVEQL QIKAVEALKN TRMVPDVSHR RLEQFTLSRK
EWCISRQRSW GVPIPALYDT NTGEALLTEE SVQHIIDVFD KSGSTDIWWE ASDDSIFVAP
KYRDNGKTYT RGYDTMDVWF DSGTSWTMLK DLPGRSTTAD SPLADIYLEG SDQHRGWFQS
SLLTSIALTG KAPYGTLITH GFALDEQGRK MSKSIGNTLE PILITNGGKD KKKYPAYGSD
VLRMWVANCE YTRDVNIGPN VIAQMSEIMR KVRTTARFML GNLQDFTYKD SVRYNDLKEI
DKYMLHELHQ FDQNVKSAFD EFSFNRAMQH VQNFTTNQLS AFYFDVIKDR VYNEKQDATS
RRMAQTVLYE IINIYTTALA PIVCHTAEEI YEHYRSKTPH PETSIFKLDN WHEQRSINSE
WNNPDLVDKW TILKNLKTEV NQVLEQARQE KAVRSSQEAE IIVSIDSKTP VGQIIHSMDD
SELASLFLTS RVQVKEGDDE VQAESVGYHR QTQLESIDCS IKAKKSSYHK CPRCWNYHAA
EIDCLCTRCE NVVSKTV
//
