ID   A0A1X6Z0H4_9RHOB        Unreviewed;       558 AA.
AC   A0A1X6Z0H4;
DT   05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT   05-JUL-2017, sequence version 1.
DT   24-JAN-2024, entry version 24.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000256|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000256|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000256|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000256|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000256|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000256|HAMAP-Rule:MF_01543,
GN   ECO:0000313|EMBL:SLN37110.1};
GN   ORFNames=PSM7751_01623 {ECO:0000313|EMBL:SLN37110.1};
OS   Pseudooceanicola marinus.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC   Paracoccaceae; Pseudooceanicola.
OX   NCBI_TaxID=396013 {ECO:0000313|EMBL:SLN37110.1, ECO:0000313|Proteomes:UP000193963};
RN   [1] {ECO:0000313|EMBL:SLN37110.1, ECO:0000313|Proteomes:UP000193963}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CECT 7751 {ECO:0000313|EMBL:SLN37110.1,
RC   ECO:0000313|Proteomes:UP000193963};
RA   Afonso C.L., Miller P.J., Scott M.A., Spackman E., Goraichik I.,
RA   Dimitrov K.M., Suarez D.L., Swayne D.E.;
RL   Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000256|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000256|HAMAP-Rule:MF_01543}.
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DR   EMBL; FWFN01000003; SLN37110.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1X6Z0H4; -.
DR   OrthoDB; 9761733at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000193963; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW   Ligase {ECO:0000256|HAMAP-Rule:MF_01543, ECO:0000313|EMBL:SLN37110.1};
KW   Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW   One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW   Rule:MF_01543}; Reference proteome {ECO:0000313|Proteomes:UP000193963}.
FT   BINDING         67..74
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   558 AA;  59077 MW;  8388CA0134D210FB CRC64;
     MSQPSDIEIA RAARKKPITE IADRLGIPEE AVLPYGRDKA KVDRDFIASL ADRRRGKLVL
     VTAVTPTPAG EGKTTTTVGL GDGLARIGEN VAICLREASL GPCFGMKGGA AGGGHAQVVP
     MEEMNLHFTG DFHAITSAHN LLAAMIDNHI HWGNALDIDA RRVTWRRVMD MNDRALRDVV
     VSLGGVGNGF PRQTGFDITV ASEIMAILCL ARDLDDLQAR LGRIIIGYTR DKAPVTAKDL
     KADGAMVVLL REAMQPNLVQ TLENTPAFVH GGPFANIAHG CNSVVATDTA LRLADIVVTE
     AGFGADLGAE KFLDIKCRKA GLSPDAAVLV ATVRSLKMSG GVDKADLGAE DAKAVARGCA
     NLGRHLENLR GYGLPVLVAI NRFDGDTEAE LAAVRDYVEG QGAEVHLCTH WADGGAGIEG
     LARRVAEVVQ AGDADFAPIY PDDMGLVEKI ETVARRIYRA EGVDMPAPVR AQLADWEKAG
     FGDLPVCMAK TQYSFSADPG LRGAPEGFRV PVREVRLSAG AGFVVAICGE IMTMPGLPRV
     PAAEAIGLNE AGEIEGLF
//