ID A0A1X7AFQ7_9GAMM Unreviewed; 3988 AA.
AC A0A1X7AFQ7;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE SubName: Full=Polyketide synthase PksN {ECO:0000313|EMBL:SMA38449.1};
DE EC=2.3.1.- {ECO:0000313|EMBL:SMA38449.1};
GN Name=pksN {ECO:0000313|EMBL:SMA38449.1};
GN ORFNames=EHSB41UT_00869 {ECO:0000313|EMBL:SMA38449.1};
OS Parendozoicomonas haliclonae.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Endozoicomonadaceae; Parendozoicomonas.
OX NCBI_TaxID=1960125 {ECO:0000313|EMBL:SMA38449.1, ECO:0000313|Proteomes:UP000196573};
RN [1] {ECO:0000313|EMBL:SMA38449.1, ECO:0000313|Proteomes:UP000196573}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SB41UT1 {ECO:0000313|EMBL:SMA38449.1};
RA Afonso C.L., Miller P.J., Scott M.A., Spackman E., Goraichik I.,
RA Dimitrov K.M., Suarez D.L., Swayne D.E.;
RL Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005194}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP
CC synthases family. {ECO:0000256|ARBA:ARBA00008467}.
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DR EMBL; FWPT01000002; SMA38449.1; -; Genomic_DNA.
DR OrthoDB; 9766402at2; -.
DR UniPathway; UPA00094; -.
DR Proteomes; UP000196573; Unassembled WGS sequence.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd05274; KR_FAS_SDR_x; 2.
DR CDD; cd00833; PKS; 3.
DR Gene3D; 3.30.70.3290; -; 2.
DR Gene3D; 3.40.47.10; -; 3.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 3.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 2.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF37; FATTY ACID SYNTHASE; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 2.
DR Pfam; PF00109; ketoacyl-synt; 3.
DR Pfam; PF02801; Ketoacyl-synt_C; 3.
DR Pfam; PF08659; KR; 3.
DR Pfam; PF21089; PKS_DH_N; 1.
DR Pfam; PF00550; PP-binding; 3.
DR Pfam; PF14765; PS-DH; 2.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00822; PKS_KR; 3.
DR SMART; SM00825; PKS_KS; 3.
DR SMART; SM00823; PKS_PP; 3.
DR SMART; SM01294; PKS_PP_betabranch; 1.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 5.
DR SUPFAM; SSF53901; Thiolase-like; 3.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00606; KS3_1; 3.
DR PROSITE; PS52004; KS3_2; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000313|EMBL:SMA38449.1};
KW Fatty acid biosynthesis {ECO:0000256|ARBA:ARBA00023160};
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00023160};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023160};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000196573};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:SMA38449.1}.
FT DOMAIN 768..843
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 874..1299
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1963..2042
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2066..2493
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 3452..3532
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3562..3986
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
SQ SEQUENCE 3988 AA; 433883 MW; 85F2C8092D091701 CRC64;
MPREKPITST LDLSESQYSY IREHRVLKVP VVPATVWLSS LSALGAMFWQ TLGLRLSAID
LLKLQALYSD NTFTLSLSDC TENELAKECS VKADKQGDIL ARLRMQPLSK GNSSSDSRLF
PFLSGIPDQA ETLQSSTVYE RLRQLSIQWG TNWEWLQQCY LYNEAVVCRI AAPSSEDYQQ
APLHPVLLDN AFASGLLINN THDSKSPLLP VHIESIVIYQ PAQGPVWSEF RLRSSDSSAP
GEPVVADITL WDLQGECLAE ISGFTSRHIP HSQLYTLFSR PVFRVVDAVL AIEEDTSPAT
CCDVQFSPLA ESDELFCQQI AEQAKADGDS PVIQPVQLES GQEGTTDHIQ LIITQLPESD
DPDICHVVLQ RVGDWLKRLI ISDKANRSAE APLFLYLGMS HAPLDLPLVV AVLSLVRVFN
REHPRCCVKV LVGDISVEWP VIRHSLINWE QELGSEHMLW LDKKQLRIPD CQKIPEVMSD
RPDNDVQTPL LPENSSVLVT GGTGGIARSL IPWLAHTTGA RHITLLSRHP IDEDQLTQLV
EACGKGCQIT HLCCDISDHS SLKAVLDNLP SEWPLKAVFH TAGINEDKTF AELDDASLAR
VFSGKAIGAW HLHQLTQDKN LQWFMLFSSL ASVFGADGQA SYAAANGFLD GLARLREKDG
LPVHTINWGP WADEGMAAST GAVMRQRMRD RGLEEMPAGI GLMQLETILA QPSGCYLAAA
FTSVSGSEGL SRLEKHLVEY LSDDRQVDVG TSQAPEKQPA LDHTTSASVL MELVRGAVID
ALKIPDASQL ENHRSFQRYG LDSLLALDIR NRLQKSIPGA LPSTLLFEYP TINTLVGYLQ
ERLQEQLAGV KPAPAEQRPA PQRLSEVSHP KNFDEPVAVI GMGFRFPGDV NDEQSFWELL
SEGRDAIREV PPERWDIDAF YDPDPSAIGK MRTRCGGFLK DIDQFDAGLF DIPAEEAEAM
DPQHRLLLET SWEAFENAGY TLAKLAGSRT GVIVGLMFNE YLHRYGNHYE AIDGFFGSGN
ADSVASGRLS YFYGLEGPSL TIDTACSSSL VAIDLALKQL HSGQSDMVLA GGVSLNLSPA
SFIEFSRLDG LAPDGRCKTF SDQADGVSWS EGCGLVLLKR LSDALRDGDP IDGVIRASAV
NQDGHSQGLT APNTRAQRKV IETCLESAGL TIDDIDYIEA HGTGTPMGDP IELQALGQVC
TRRTADRPLM IGSVKSNFGH TQAAAGLAGL IKTLLAIKHK TLPSSLHCDQ KTSAFDWQAQ
PLRVVTTPTF WPEIGNRPAR AGISSFSISG TNAHILVEEP PLPEPVKEAD NGHGLPLVLS
AHTQAALDLA LVHLQQFVRQ NPAISLMAIA RTLALAKTPL PVRRAWLVQS RDGLLNLNNE
NSLPAQDFPE PIRPLVERYL QGESVDWQPV FQAHTPCVRL PTISFQRQRY WKVFADEPAD
SQPAKQTSSN DHYTLEWEPL EPEWPSVREG IPQNWQVFGD AESLQSPLFR NLERLAKNRD
VTLVINPDED SGRSPDCILV VSLTGNIDNP LEASPQWPDL AGVIKPLQHC VQEQLGAGRK
ELPVWLLTVQ ACQVHKGESV IPDNSRFRGV HLALQAEYPE ISSRWLDIPA DTDDELWQQA
LDCLCTPLSS QRLFECQALR PGPAGLVQWW QQVLCPVERT PLSIDLPETI LITGGLGALG
LAVAEYLGKR LAEQQKQGCL ILVSRRAGPD EVAQQRLDAI GQAGCDVHYY QADVADQEQL
SGLFLQLGQQ GQLPDLIIHC AGVLDDGLLT NLTDQQWQTV LRPKIDGAWN LHCLTREWPE
TRLVMFSSIS SVFGNIGQAN YMTGNCYLDA LAQYRTSHGL PTLSLAWGGW EGEGMSARIG
SIESQESSVV TLLNPKRALH TMEKALATLE QNVTPSPVHV IAAINWSGMA QRFTGKPVPP
FLHHMLSQQN LVSEQPLPEA VKPAKQTAYT AQLLSMEEAA RREHLLDWLY LQVLDLLGVA
PDSHEALARD SLTGLFEMGL DSLKAVAFGQ RMEKQLGLEL PATLAVTVPN LQALADRILA
ELVKVVGSES VPEAAALPKM AVNDSEQPIA IIGVGCRLPG GVHDLSSLWE MLEQGSDTTC
DIPEDRFDIN PLFDTDPNAT GKSYTRRGAF IEEPGRFDAG FFGIPPREAE SMDPQHRLLL
ESAWTALENA GLNPEQLHDS ETGLYVGIGP SEYEQLQDRG LDNLNALDVT GTHTSFAAGR
LSYWLGLQGP SLAVDTACSS SLVAIHLAIQ ALRNGECDLA LAGGVQLLLS PAGFVALSRT
RAIAPDGRCK TFSDQADGYG RGEGCGMVAL RRLDEAQRRG EPVLAVIRGS AVNHDGSSSG
MTVPNGLAQQ KVLRQALDNA GVPADSVDFV ETHGTGTPLG DPIEVDALQA VYGQAPGRIQ
PLRIGSIKAN IGHLESAAGV AGLLKLVAIL NRRALPPHPL DGPLNTRINW SDISVVVNSE
LRPLLNNDSR QSLRCGLSSF GISGTNVHLI MEQAPPASTA ANDEEQPVLV LLSGKNKAAL
QRRIQQLAAQ LRQADSVTDS LQGLAYTLAV GRAHYPWRAA IICSSVEQLQ AGLEALSEGR
AGEGCFLSEA VNVNGTPSTQ PLPEQPLATE SLPLMAEYFC QGRALDWAEI YRALGIQARL
VNLPGYPFQG EHFWIQSDRL RSHFSSSQPN DEVIPATGRY PFSGQRIPLS QSGDSLYQLT
ISESETPFLY HHKVYGEVAL AGAVHISFML AAASDMLATD QLLLTSISFT EPMLPASRDT
LYCRLTPVGV GDSRMHCRVY SEHQGGLREH AQGYVEPLFT QVSSQTESGV LMPTLENAPN
FSVVEWLRCL DQYFGIHFDP LWCWGQGLQA TGSRVLNRIQ GPDAAAGIAP LHPVLLDNAI
ASGAILMAEQ EDAASRYIPL SIERLTLYGA APVRALSDYQ LLSDNDTVKG DSDLSTRRAN
FSLRDKQGHC VVEVEGFACK QTDRDTLLRQ HSEAEAKASG AGPALWTVGW QPAGEHSLSE
NSPSRDNNAI QIVVLDKSDT RNALADCTDE VDANESVTVV FWPGQESTEE RDFVPEELEQ
LTQKALVWLK VLADEQTTAR HVWIFPVQNN SSSCYPVSAP VLAMARSAQR EHPQLPLTCL
LLSATVEARQ HLLSQVHWLT GQTLPRECLI TIEGDIARVA VPELRVKPES ANSTPVSLEG
ETLLVTGAFG SLAQHILPEL MEWLRPSCVV LLGRHPPNVE AKTVIAGVES RGATVICKQC
DISDEQAISA QIAAIPVEYP LTAIFHLAGE LQDALLTDTQ PEQLEKALSG KANGAWWLHI
ASLSGPKPLK YFVLFSSAAA VLGSPGQTAY SAANGFLDAI GVYRRQQGLA CQVIHWGPWQ
STGMAGGLSQ QDLQRLHSMG ITPLASEQAL VLLKQVLAAD DRDPVIAMDY QPQTLRQSSM
LQRPIVDNKQ PVSGTTGDAV HLLSLPDDEF LASLTTMVKK ALMPLLALPD DQSFIDVHQP
LQSLGLDSLL AVELRNTLMT QLSSVLDGGL PATLAFDYPT LHKLVAHLAD RMSSRRARMH
TEAKTKSVQS QATRNALRPK ESGKIAVIGL GCRYPSGIQN PEGLWAFLQQ DESVAREVPP
SRWDINAYYD PVPGTPGKTM TRNGGFIDQV DLFDADFFGL TDHEADLMDP QQRILLETSW
EAFERAGYPL TRLRGSRTGV FMGLMSSDYG LLLRQGAETL DGSIASGNSP SLISGRLSYF
FDLQGPSLTV DTACSSSLVA LELACQKLRA GQCDLALAGG VSLNLSPVMY VEFSSLEGMA
PDGRCKPFSA SADGVGWGEG CGVAVLKRLD DAIEDGDTIL SVIAGCSSNH DGRSQGVTAP
NGPAQVQVIR AALDDANLNP EAIDLIEAHG TGTRLGDPIE ARALEQVFRN GPDHRLFLSA
VKHNLGHTQA AAGISGLIKV TLCLNHEQIP GSSSFSQLSG EVDWEHSPLI PAADSQVWSS
SPLRKRRAGI SSFGISGTNA HIVVEEAP
//
