ID A0A1X7AFQ9_9GAMM Unreviewed; 4030 AA.
AC A0A1X7AFQ9;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE SubName: Full=Erythronolide synthase, modules 5 and 6 {ECO:0000313|EMBL:SMA38199.1};
DE EC=2.3.1.94 {ECO:0000313|EMBL:SMA38199.1};
GN Name=eryA_1 {ECO:0000313|EMBL:SMA38199.1};
GN ORFNames=EHSB41UT_00852 {ECO:0000313|EMBL:SMA38199.1};
OS Parendozoicomonas haliclonae.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Endozoicomonadaceae; Parendozoicomonas.
OX NCBI_TaxID=1960125 {ECO:0000313|EMBL:SMA38199.1, ECO:0000313|Proteomes:UP000196573};
RN [1] {ECO:0000313|EMBL:SMA38199.1, ECO:0000313|Proteomes:UP000196573}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SB41UT1 {ECO:0000313|EMBL:SMA38199.1};
RA Afonso C.L., Miller P.J., Scott M.A., Spackman E., Goraichik I.,
RA Dimitrov K.M., Suarez D.L., Swayne D.E.;
RL Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005194}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP
CC synthases family. {ECO:0000256|ARBA:ARBA00008467}.
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DR EMBL; FWPT01000002; SMA38199.1; -; Genomic_DNA.
DR OrthoDB; 9766402at2; -.
DR UniPathway; UPA00094; -.
DR Proteomes; UP000196573; Unassembled WGS sequence.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0047879; F:erythronolide synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd02440; AdoMet_MTases; 1.
DR CDD; cd05195; enoyl_red; 1.
DR CDD; cd05274; KR_FAS_SDR_x; 1.
DR CDD; cd00833; PKS; 4.
DR Gene3D; 3.30.70.3290; -; 2.
DR Gene3D; 3.40.47.10; -; 4.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.90.180.10; Medium-chain alcohol dehydrogenases, catalytic domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 3.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR013154; ADH-like_N.
DR InterPro; IPR011032; GroES-like_sf.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR013217; Methyltransf_12.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR020843; PKS_ER.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR002364; Quin_OxRdtase/zeta-crystal_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF37; FATTY ACID SYNTHASE; 1.
DR Pfam; PF08240; ADH_N; 1.
DR Pfam; PF13602; ADH_zinc_N_2; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 4.
DR Pfam; PF00109; ketoacyl-synt; 4.
DR Pfam; PF02801; Ketoacyl-synt_C; 4.
DR Pfam; PF08659; KR; 2.
DR Pfam; PF08242; Methyltransf_12; 1.
DR Pfam; PF00550; PP-binding; 3.
DR SMART; SM00829; PKS_ER; 1.
DR SMART; SM00822; PKS_KR; 2.
DR SMART; SM00825; PKS_KS; 4.
DR SMART; SM00823; PKS_PP; 3.
DR SMART; SM01294; PKS_PP_betabranch; 2.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF50129; GroES-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 5.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR SUPFAM; SSF53901; Thiolase-like; 4.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00606; KS3_1; 2.
DR PROSITE; PS52004; KS3_2; 4.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
DR PROSITE; PS01162; QOR_ZETA_CRYSTAL; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000313|EMBL:SMA38199.1};
KW Fatty acid biosynthesis {ECO:0000256|ARBA:ARBA00023160};
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00023160};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023160};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000196573};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:SMA38199.1}.
FT DOMAIN 1..439
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 611..685
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 699..1114
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1943..2020
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2042..2474
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 3516..3591
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3607..4026
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
SQ SEQUENCE 4030 AA; 435476 MW; C3F6CFDEFC0495F4 CRC64;
MQYAIGGIAA LLPGDEDRSD QALNNRSKWN CLSNSNIYSV DRCIRDCPPN RIPDHLSDKD
MFQLWSQYGK RAGWLENYDH FDAGFFQLSD AEATGMDPRQ RLMLQTAYHA LEQSGLTNTA
LTDCKTGVFV GASQDDFSCC LAGSKEQQPF LNPDAKPVTG DNRAMLAGRI AHALNLSGMA
CVVDTASSSG LSALALACRS LQAGECDQAL VGSVQLNLDP LYTCRLGLAG MLSPKGRCAS
FSTQADGYVR AEGCIALVLM PLERALAEKR PINGIIRGLA TNHDGRTKIP TSPDQAAQQA
VIQQALTDAG CLPEDIGYLE THGSGTPVGD PIELAALQQV FSHSEAARGH ALPLGAVKAQ
IGHTEAVAGL AGLVHALAVL KSHERPASAL DASHSLLAGG DYPGLQLSQT PIVWGAGNKL
AGVSAFGISG TNVHVIVEAA QSESDTVVPS QAPAQDKDDN EILLPVSAKT PEALKQQLDN
IQHWLLQNPT ISLQDVMYTL AVGRESFDCR VLITGRNREE LLEQLSRYQN PQLLHPPHDV
PAVKDQRSNS LQALAANWRD GHSVDWTLAL DSGRIVSLPG YPFQTRAFPT PSLQRRPSEQ
QWQAPILLSH DEISANVLTI CASVLGIDRN DLKPDIALRD QGLSSILTQV LHQKLESLCA
GTLPKTLLFN YQTPAAVIGY LQKTGVSPST KETNQQVKDE RIAIVGIGCR FPGSINNPEQ
FWELLISGKD GIEPLTNQRW AASFNRELPQ GAGLLDDISH FDAAAFGIPE SALEEMDPQM
AVLFQVVQQA LTDAGLTRDQ LRGSRTGVFI GISSNEHLSR RMQGDITANT LLFTAHSAMV
GRLSYWLDLH GPNLAIDTAC SSSLVAIHKA CQSLRAGECD LAIAGGVNLL LDPATTFYYQ
KLGVLSPTSR CRTFSTEADG YVRAEGSGIV ILTRCNNAGV ETALHTQAPR GFIAGSAVNH
DGYSQGMTAP NGLAQQAVIH SALQQAGCGA GDIGYAEAHG TGTALGDPVE VEALNAVYGQ
GRDADRPLLL GAVKSNIGHT ESAAGVAGLI KVVLALEHGE LPANLNSQPL SSAIPWEELS
LKVVSENQPG PASRWACVSS FGFSGTNAHA IIQRASIEEG QSQAIVRPYS PPVVTASDAV
PELGWKPLGV SFDSHPPSLY NLSWELCGEA GPWPSEMDLS ISEENDHLVI WTLSGTRDTA
RQQVMAAIDK ALAMLQMAIK RQEPSQPLVW LLTEDDNEAS RLMVAALQGL SRVFVAEHSE
QSFYCITVAH NQGAIEACLK DRPAESVVIW KENGFHGLRL RSASPEFGLE TQQRKLIIQQ
PGQLGGFGIT SSPRQSPEAG EVEIAVRCAG LNFRDVMNIL GLYPGEAGEP GLEVSGTVSR
VGEGCQRLKS GDAVYGLAPG GFSQYTTTNE QYLCRKGEWL DYSQAAGIPV VWLTAWHALV
TLGNLQAGET VLIHCAAGGV GMAAVQIARF RGARVIATAS ARKWDALREM GVEHLFSSRD
TGFYQGIREL FPDNGESGVD LILNSLTGDI LDRSLDLLTA QGTFIELGKV DIRSAEWLAE
HYPEISYHHF DLMSVAAANP DHITATLQHL NGLFEQKVFQ PLPVQTFPLS DSEKALRFMA
GAHHIGKLVL ENDLGSSRSS IQTTSEPIAL SPHAEEIIVI SGGTGAMGIH LAGWLRTQVS
ATLLLVGRKP LTSKQQSLLG QWQTDADRKS DSKAATKASV RFWQGDISSP EIADALCDEI
RPQGRVIAVY HLAGVIEDCA LIKANPEQFE RVMAGKVWGA WNLHHRLVRY WPAANLVLFS
SVSGLFGIPG QGSYVTANAS LEGLANLRRA QSLPVQIIYW GHWAGTGMAS DMTESQWHYI
HQQGLQALDP DQALDAWEPV LAQPEQSTSV AILSLDLQSD KVTALPLIKP LLPEEVSSEA
KALSCSDKGG EKGDQDNQQA IEPTREHLQQ LIISGLQRIL PGGASHLDMG RPLEGQGLDS
LLGVEFRRYL NNKLALNLPA SLLFDYPTIK TLIDHLMETL DSQSDNRGAS DSPSPDAIKG
KKDAIAIIGM GCRFPGGVTS PEQFWQLLEQ GTDAITSIPD RGWEPEAFYS HEAGTPSRMI
CRAGGFLDHI YDFDNEFFEV LPKEATYLDP RLRLFMEVAW EALQRAGQTC TGLLNSMTSV
YAGFCGSEYQ STVMADADTI TPYSLLGTSF SAMAARLSYW LGLNAANLAI DTACSSSLVA
VDLACQSLLQ GHADLAIAGG ANLILGPESS IYFSQLKALS PTDRCHSFGD KADGYVRAEG
CGVVVLKRLS QALADGDRVD GVIRATAVNH DGQSQGPTAP NGRAQSRLLQ SALQSAGVNA
EQVAYIEAHG TGTPLGDPVE TNALADVFAS PDKSLLIGSV KSNIGHTEAA AGIAGLIKTV
LCLQKRYVPP SLNAQQVNPR CTALLPHSDN GEEKPPSLKV VTAGTSLPDA DQPVLAGVSA
FGFSGTNAHV LVESPPHNKQ SEQPSFAETV LIILSAKTPR ALMMQAVNLQ QYIDEYPATN
LEQLAHSLAL TRDGFAWRLA FLADSLADIN QKLEQYLSLV RSDSAPDKTT AVTEDKWDGL
WQSSPESDMP ERHRDNVPVA PSLALTTLAD LFTQGYDLDW SVVYPNVRRP LLRLPVYPYN
KTTFRFQSHP QKQPSYKQQA EPRHPQGFQL KGRCWTQYPL SATDRPDEFP IDQENQLLEI
LNQLSVRAIM EALPVLGVET TPGCTFDPEV FPDPLSAHIP RRRLYRRLLT ILRNNGYCLP
VADGHSWQWQ EPHQAASVNT LIPEQQKSHP AVRLFKRCAP QLGQVLSGTV DPLSLVFPDG
DSQDVMGIYR DMATSRLINQ QVAQQVAALA RGLSGPVRIL EIGAGTGSTT ESVLRELADT
PVHYTFTDIG QRFVTMARSS LIGLDNPNHR FTFETLDISQ NPAGSPVSQL GPYDIVIAVN
VLHATPDIQQ CLKHIRTLCA PSGQFIFVEM LKPVPWLDLI FGLLDGWWCF TDHPQRTEHP
LINASQWFEQ LSAAEFHGGR VVPVPGSGAF QKLLMASASD VVLLGDPEQG ADKDTRHWLC
ICSSATDVNG ITGQLTASGE SCEWLQLESV ANAQSLMERA PTDILYFPAV DGEGLLEQVW
SACEPLLIFL RRLLKVEHDL DSTAVEVRSF WMITRGAQPF DDETVPDSLS ASLALSCTAG
LLRAIHQEMP EIHCRHLDFS AESSLPQQRP QLIETILSES ILSEPMLILH WHKGECYRLT
MDSLPAESPR AVTLTKNGSY LITGGFGAVG QAWMEYLVEH GARTLILLGR NSPSQSVEKR
LASLRADGIT IHCVHGDIGQ PQTLAELFKK DWLQAGDGLK GVIHAAGTIH DITLAGLSWE
VMADVLSAKA LGAWQLHQLT CGRALDFFVL CSSVVTVAGG PGQGAYIAAN LLLDTLAEFR
IRSGLPACSL NLPVIPDFGM AARHPPRPEA LAAFARDGVY PMQASSLSVL LAQTMAQSHC
GQLMVYPEPA ASSVTADGIK TDTRQTGIDD SRSKDKLTRS ELDRLLCQSL GWPEDHQLSP
RQSFREMGLD SLVAVEIRSR LMKRVGRPLP ISLLYDFPTP ERLYSFLTEN ADDPAPNSKP
CTSTAPDRRI AIIGMGCRYP GGADSPEEFW ELLKSGTDAV GPVPKSRLNT MTGTGFSNIE
GGFLENIYLF DAGFFGIAPA EARELDPQLF VMMQTAWNAL ERAGQRRETL YGSDTGVWIG
LYANGHQGRM LNLPDSAINA TNLINSEHSA MVGRLSYSLG LQGPNMPINT GCSSSLVAIH
QACLGLLNDD CSLAVAGGVS LIERPGHYPM LARMNVLSPR FRCRPFAADA DGFVPAEGCG
MVVLKRLEDA RRDGDPVLAV ISASAINQDG HSQGPTAPNG EAQIQLLRKT LKRTGLEGSD
IDYMEAHGTG TRLGDPLEAR AVGEVYGRGR RDSELLWMGS AKGNIGHSEA AAGVAGVIKT
VLAMNHGSIP ASLHCSELSP EIPWHSLNLT VPREQIPWPV NTKDKRRAAV SAFGISGTNA
HLILEEPDHE
//
