ID A0A1X7H8L5_9PROT Unreviewed; 638 AA.
AC A0A1X7H8L5;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=Nif-specific regulatory protein {ECO:0000256|ARBA:ARBA00015308, ECO:0000256|RuleBase:RU368029};
GN ORFNames=SAMN02982917_5238 {ECO:0000313|EMBL:SMF81715.1};
OS Azospirillum oryzae.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Azospirillaceae; Azospirillum.
OX NCBI_TaxID=286727 {ECO:0000313|EMBL:SMF81715.1, ECO:0000313|Proteomes:UP000192936};
RN [1] {ECO:0000313|EMBL:SMF81715.1, ECO:0000313|Proteomes:UP000192936}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A2P {ECO:0000313|EMBL:SMF81715.1,
RC ECO:0000313|Proteomes:UP000192936};
RA Afonso C.L., Miller P.J., Scott M.A., Spackman E., Goraichik I.,
RA Dimitrov K.M., Suarez D.L., Swayne D.E.;
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Required for activation of most nif operons, which are
CC directly involved in nitrogen fixation. {ECO:0000256|ARBA:ARBA00002167,
CC ECO:0000256|RuleBase:RU368029}.
CC -!- SUBUNIT: Interacts with sigma-54. {ECO:0000256|ARBA:ARBA00011135,
CC ECO:0000256|RuleBase:RU368029}.
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DR EMBL; FXAK01000007; SMF81715.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1X7H8L5; -.
DR STRING; 286727.SAMN02982917_5238; -.
DR OrthoDB; 9770562at2; -.
DR Proteomes; UP000192936; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-UniRule.
DR CDD; cd00009; AAA; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 3.30.450.40; -; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003018; GAF.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR InterPro; IPR002197; HTH_Fis.
DR InterPro; IPR010113; Nif-specific_regulatory_prot.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR002078; Sigma_54_int.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR InterPro; IPR025944; Sigma_54_int_dom_CS.
DR NCBIfam; TIGR01817; nifA; 1.
DR PANTHER; PTHR32071; TRANSCRIPTIONAL REGULATORY PROTEIN; 1.
DR PANTHER; PTHR32071:SF95; TRANSCRIPTIONAL REGULATORY PROTEIN ZRAR; 1.
DR Pfam; PF01590; GAF; 1.
DR Pfam; PF02954; HTH_8; 1.
DR Pfam; PF00158; Sigma54_activat; 1.
DR PRINTS; PR01590; HTHFIS.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00065; GAF; 1.
DR SUPFAM; SSF55781; GAF domain-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE 4: Predicted;
KW Activator {ECO:0000256|ARBA:ARBA00023159, ECO:0000256|RuleBase:RU368029};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU368029};
KW Nitrogen fixation {ECO:0000256|ARBA:ARBA00023231,
KW ECO:0000256|RuleBase:RU368029};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Transcription {ECO:0000256|RuleBase:RU368029};
KW Transcription regulation {ECO:0000256|RuleBase:RU368029};
KW Two-component regulatory system {ECO:0000256|ARBA:ARBA00023012,
KW ECO:0000256|RuleBase:RU368029}.
FT DOMAIN 207..435
FT /note="Sigma-54 factor interaction"
FT /evidence="ECO:0000259|PROSITE:PS50045"
FT REGION 478..522
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 565..593
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 565..583
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 638 AA; 68219 MW; 1D69C976C4E4A09C CRC64;
MPSAAQSASS SSLELLTIYE VSKILGSSLD LEQTLREVLR ALSYQLQMHR GRVYLQGEDG
ALRLVAAQGL PKDSPAQEID YNQGEGISGR ILKTGMPAVV PNLAEEPLFN NRSGGRDDLD
EQVASLVGVP IKAAGTVIGV LTIDRISDDG PSGHFGSDVR FLTMVANLIG QTVRLHRTVA
EERRFMMRET FRVQKELRPV VAPVNDVVCT SPNMVDVLAQ VHRVAPFKST VLIRGESGTG
KELIARAIHN LSPRKDAPFI RVNCAALPES LLESELFGHE KGSFTGAQKD HKGRFELASG
GTLFLDEIGD ISSNFQAKLL RVLQEQEFER VGGSKTIKTD VRLICATNLN LEEAVGHGKF
RADLYFRINV VTIHLPPLRE RRGDIATLAK HFVAKFGRDN GLNLDIAAEA LEVLNRCTWP
GNVRELENCI ERAATQCRNG TIRVPDLSCS MNLCNSSVLF QYRTMGAAVG GLAPSMSGTP
TNPAAGNPGV GNPAQGRAQP APMNGSAMPP AVPAPANGAP NGAPNGAQWP ACASGCGAGP
MPVCGASKPL PPSAIVPLPA PQPAVSAPPI PSAPAPQPAA AGPDLPLDEP ESGPLRDRLV
WAMERTGWVQ AKAARLLGMT TRQVSYALRK YNIEIKRF
//
