ID A0A1X7J7V6_9CORY Unreviewed; 165 AA.
AC A0A1X7J7V6;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=tRNA threonylcarbamoyladenosine biosynthesis protein TsaE {ECO:0000256|ARBA:ARBA00019010};
DE AltName: Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE {ECO:0000256|ARBA:ARBA00032441};
GN Name=tsaE {ECO:0000313|EMBL:NLP38722.1};
GN ORFNames=GX356_03230 {ECO:0000313|EMBL:NLP38722.1}, SAMN06295981_1336
GN {ECO:0000313|EMBL:SMG23824.1};
OS Corynebacterium pollutisoli.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
OC Corynebacteriaceae; Corynebacterium.
OX NCBI_TaxID=1610489 {ECO:0000313|EMBL:SMG23824.1, ECO:0000313|Proteomes:UP000193309};
RN [1] {ECO:0000313|EMBL:SMG23824.1, ECO:0000313|Proteomes:UP000193309}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VDS {ECO:0000313|EMBL:SMG23824.1,
RC ECO:0000313|Proteomes:UP000193309};
RA Afonso C.L., Miller P.J., Scott M.A., Spackman E., Goraichik I.,
RA Dimitrov K.M., Suarez D.L., Swayne D.E.;
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:NLP38722.1, ECO:0000313|Proteomes:UP000568696}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AS23ysBPME_344 {ECO:0000313|EMBL:NLP38722.1};
RX PubMed=32123542;
RA Campanaro S., Treu L., Rodriguez-R L.M., Kovalovszki A., Ziels R.M.,
RA Maus I., Zhu X., Kougias P.G., Basile A., Luo G., Schluter A.,
RA Konstantinidis K.T., Angelidaki I.;
RT "New insights from the biogas microbiome by comprehensive genome-resolved
RT metagenomics of nearly 1600 species originating from multiple anaerobic
RT digesters.";
RL Biotechnol. Biofuels 13:25-20(2020).
CC -!- FUNCTION: Required for the formation of a threonylcarbamoyl group on
CC adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning
CC with adenine. Is involved in the transfer of the threonylcarbamoyl
CC moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37,
CC together with TsaD and TsaB. TsaE seems to play an indirect role in the
CC t(6)A biosynthesis pathway, possibly in regulating the core enzymatic
CC function of TsaD. {ECO:0000256|ARBA:ARBA00024908}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the TsaE family.
CC {ECO:0000256|ARBA:ARBA00007599}.
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DR EMBL; JAAYSN010000079; NLP38722.1; -; Genomic_DNA.
DR EMBL; FXAR01000004; SMG23824.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1X7J7V6; -.
DR STRING; 1610489.SAMN06295981_1336; -.
DR OrthoDB; 9800307at2; -.
DR Proteomes; UP000193309; Unassembled WGS sequence.
DR Proteomes; UP000568696; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0002949; P:tRNA threonylcarbamoyladenosine modification; IEA:InterPro.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR003442; T6A_TsaE.
DR NCBIfam; TIGR00150; T6A_YjeE; 1.
DR PANTHER; PTHR33540; TRNA THREONYLCARBAMOYLADENOSINE BIOSYNTHESIS PROTEIN TSAE; 1.
DR PANTHER; PTHR33540:SF2; TRNA THREONYLCARBAMOYLADENOSINE BIOSYNTHESIS PROTEIN TSAE; 1.
DR Pfam; PF02367; TsaE; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Transferase {ECO:0000313|EMBL:NLP38722.1}.
SQ SEQUENCE 165 AA; 17797 MW; 33E1508B3D317952 CRC64;
MTETFPPEGT RELATAEDTR AFAEDMGRSL QAGDVVILDG PLGAGKTTFT QGLARGLQVK
GRVTSPTFVI AREHRSTVGG PTLIHLDAYR MLGEGSSGDP LGELDALDLD TELADAVVVA
EWGGGLVEQI AERYLLVQFD RETAVIRDPG SEARIISWRW VEQAD
//
