ID A0A1X7M444_9BURK Unreviewed; 640 AA.
AC A0A1X7M444;
DT 05-JUL-2017, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2017, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE RecName: Full=Glutathione import ATP-binding protein GsiA {ECO:0000256|ARBA:ARBA00041187};
DE EC=7.4.2.10 {ECO:0000256|ARBA:ARBA00039050};
GN ORFNames=SAMN06265784_11817 {ECO:0000313|EMBL:SMG60831.1};
OS Paraburkholderia susongensis.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Paraburkholderia.
OX NCBI_TaxID=1515439 {ECO:0000313|EMBL:SMG60831.1, ECO:0000313|Proteomes:UP000193228};
RN [1] {ECO:0000313|Proteomes:UP000193228}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LMG 29540 {ECO:0000313|Proteomes:UP000193228};
RA Varghese N., Submissions S.;
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Part of the ABC transporter complex GsiABCD involved in
CC glutathione import. Responsible for energy coupling to the transport
CC system. {ECO:0000256|ARBA:ARBA00037530}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + glutathione(out) + H2O = ADP + glutathione(in) + H(+) +
CC phosphate; Xref=Rhea:RHEA:29791, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57925, ChEBI:CHEBI:456216; EC=7.4.2.10;
CC Evidence={ECO:0000256|ARBA:ARBA00036581};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (GsiA),
CC two transmembrane proteins (GsiC and GsiD) and a solute-binding protein
CC (GsiB). {ECO:0000256|ARBA:ARBA00011469}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Glutathione
CC importer (TC 3.A.1.5.11) family. {ECO:0000256|ARBA:ARBA00038416}.
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DR EMBL; FXAT01000018; SMG60831.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1X7M444; -.
DR STRING; 1515439.SAMN06265784_11817; -.
DR OrthoDB; 9802772at2; -.
DR Proteomes; UP000193228; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0015833; P:peptide transport; IEA:InterPro.
DR CDD; cd03257; ABC_NikE_OppD_transporters; 2.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR013563; Oligopep_ABC_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR43776:SF15; GLUTATHIONE IMPORT ATP-BINDING PROTEIN GSIA; 1.
DR PANTHER; PTHR43776; TRANSPORT ATP-BINDING PROTEIN; 1.
DR Pfam; PF00005; ABC_tran; 2.
DR Pfam; PF08352; oligo_HPY; 2.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 2.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000313|EMBL:SMG60831.1};
KW Cell inner membrane {ECO:0000256|ARBA:ARBA00022519};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Translocase {ECO:0000256|ARBA:ARBA00022967};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 21..277
FT /note="ABC transporter"
FT /evidence="ECO:0000259|PROSITE:PS50893"
FT DOMAIN 334..584
FT /note="ABC transporter"
FT /evidence="ECO:0000259|PROSITE:PS50893"
SQ SEQUENCE 640 AA; 68743 MW; E71F12EFB0C5BC22 CRC64;
MPTSSHTARP LIETMPPQRV LAVDDLTVAF RRGGSTFNAV RNLSLTVERG ETLAIVGESG
SGKSVTSLSL MRLIEHGGGR LAGGSIAFRR RDGSVLDLAK ASSGTMRTIR GADIAMIFQE
PMTSLNPVFT VGDQISEAIA LHQGKSREAA RAETLRLLEL VRIPEAKRVA ARFPHQLSGG
MRQRVMIAMA LSCKPALLIA DEPTTALDVT IQAQILQLIR GLQDEMNMGV IFITHDMGVV
AEVADRVLVM YRGEKVEEGA ADTLFAAPSH PYTKALLAAV PRLGSMQGTD QPARFPILSV
EEAGANGADA AVQPAAAVAD EAQPPVSDET PPVLRVRDLV TRFPVRSGVF GRVTGRVHAV
EKVSFDLRPG ETLALVGESG CGKSTTGRSL LRLVESQSGS IEFNGKEISS LTGPALQTLR
RDIQFIFQDP FASLNPRLTV GFSIMEPLLV HGVAQGAQAQ ARVAWLLEKV GLPAEAARRY
PHEFSGGQRQ RIAIARALAL NPKVVIADES VSALDVSVQA QIVNLMLDLQ RELGVAYLFI
SHDMAVVERI SHRVAVMYLG QIVEIGPRRA VFEAPQHPYT KKLMSAVPVA DPARRHAKRM
LAADDIPSPI RALHDEPVVA PLVAVGPGHF VAQHRVGGAY
//
