ID A0A1X9LHM9_9MICO Unreviewed; 809 AA.
AC A0A1X9LHM9;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=B5808_05045 {ECO:0000313|EMBL:ARJ04663.1}, GCM10010988_37780
GN {ECO:0000313|EMBL:GGI42187.1};
OS Cnuibacter physcomitrellae.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Cnuibacter.
OX NCBI_TaxID=1619308 {ECO:0000313|EMBL:ARJ04663.1, ECO:0000313|Proteomes:UP000192775};
RN [1] {ECO:0000313|EMBL:ARJ04663.1, ECO:0000313|Proteomes:UP000192775}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=XA {ECO:0000313|EMBL:ARJ04663.1}, and XA(T)
RC {ECO:0000313|Proteomes:UP000192775};
RA Afonso C.L., Miller P.J., Scott M.A., Spackman E., Goraichik I.,
RA Dimitrov K.M., Suarez D.L., Swayne D.E.;
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:GGI42187.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CGMCC 1.15041 {ECO:0000313|EMBL:GGI42187.1};
RX PubMed=30832757;
RA Wu L., Ma J.;
RT "The Global Catalogue of Microorganisms (GCM) 10K type strain sequencing
RT project: providing services to taxonomists for standard genome sequencing
RT and annotation.";
RL Int. J. Syst. Evol. Microbiol. 69:895-898(2019).
RN [3] {ECO:0000313|EMBL:GGI42187.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CGMCC 1.15041 {ECO:0000313|EMBL:GGI42187.1};
RA Sun Q., Zhou Y.;
RL Submitted (DEC-2022) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP020715; ARJ04663.1; -; Genomic_DNA.
DR EMBL; BMHD01000002; GGI42187.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1X9LHM9; -.
DR STRING; 1619308.B5808_05045; -.
DR KEGG; cphy:B5808_05045; -.
DR Proteomes; UP000192775; Chromosome.
DR Proteomes; UP000633908; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000192775}.
FT DOMAIN 44..340
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 341..620
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 703..726
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 809 AA; 88749 MW; 7BEE8EC2BEC897C4 CRC64;
MTPPSDRIRT DAADSATGPS IVPVIVDPAA GPRAAEGAPD PLLDGLNPPQ RAAVEYRGDA
LLIVAGAGSG KTRVLTHRIA SLIRNREAWP SQILAITFTN KAAAEMRERV SHLLGGIAEG
MWISTFHSAC VRILRREAES FGFTRAFTIY DSADSRALIK RLIKDLQADT YGYTVPQVAA
RISKLKNELT DAETYARQAN YSDPQDAVFS QLYQEYAHAL QAANAFDFDD LISQTVYLFR
AFPEVADRYR TRFRHILVDE YQDTNHAQYS LIRELTRPPR SNELEGVSSP GNGGASLTVV
GDSDQSIYAF RGADIRNIVE FERDFPGAEV VLLEQNYRST QNILSAANAV ISNNFDRKDK
NLWTAVGDGA KIVGYTGYSG HDEAQFVADE IAKLHDEGIA YNEMAVFYRT NSQTRALEEI
FIRSAVPYRV LGGTKFYERA EIKDAMAYLT SVANPFDALA LRRILNTPKR GIGPATETQL
AGFAEREQIS YREAMRRAPE LGLGPKVTKA IVDLATLLDE AAELADPDRP GGPGRVSEVL
TLLIERSGYV TTLRASRDPQ DEARAENVEE LLAVTKEFAV NNPGGTLVDF LTEVSLVAAV
DELDDASGTV SMMTLHTAKG LEFDAVFITG VEEDLIPHRM SANEPGGPAE ERRLFYVGIT
RARKRLFLTL AMTRAQFGET SVAMPSRYLQ EIPGDLVDWV QSPGGATSRG GTQPRALNAA
RPGSSRWNDG PVYLPPKPKT EWANRVTGQV RDNGDLELVP GDRIRHTDFG EGRVDQVTGE
GAKRVAHVTF ESAGRKKLLI KIAPIEKVG
//
