ID A0A1X9M682_9BACI Unreviewed; 469 AA.
AC A0A1X9M682;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=protein-N(pi)-phosphohistidine--sucrose phosphotransferase {ECO:0000256|ARBA:ARBA00044053};
DE EC=2.7.1.211 {ECO:0000256|ARBA:ARBA00044053};
GN Name=treB_2 {ECO:0000313|EMBL:ARK28955.1};
GN ORFNames=BkAM31D_03260 {ECO:0000313|EMBL:ARK28955.1};
OS Halalkalibacter krulwichiae.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Halalkalibacter.
OX NCBI_TaxID=199441 {ECO:0000313|EMBL:ARK28955.1, ECO:0000313|Proteomes:UP000193006};
RN [1] {ECO:0000313|EMBL:ARK28955.1, ECO:0000313|Proteomes:UP000193006}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AM31D {ECO:0000313|EMBL:ARK28955.1,
RC ECO:0000313|Proteomes:UP000193006};
RA Krulwich T.A., Anastor L., Ehrlich R., Ehrlich G.D., Janto B.;
RT "Bacillus krulwichiae AM31D Genome sequencing and assembly.";
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N(pros)-phospho-L-histidyl-[protein](out) + sucrose = L-
CC histidyl-[protein] + sucrose 6(G)-phosphate(in);
CC Xref=Rhea:RHEA:49236, Rhea:RHEA-COMP:9745, Rhea:RHEA-COMP:9746,
CC ChEBI:CHEBI:17992, ChEBI:CHEBI:29979, ChEBI:CHEBI:64837,
CC ChEBI:CHEBI:91002; EC=2.7.1.211;
CC Evidence={ECO:0000256|ARBA:ARBA00043763};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP020814; ARK28955.1; -; Genomic_DNA.
DR RefSeq; WP_066157838.1; NZ_CP020814.1.
DR AlphaFoldDB; A0A1X9M682; -.
DR STRING; 199441.BkAM31D_03260; -.
DR KEGG; bkw:BkAM31D_03260; -.
DR Proteomes; UP000193006; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR GO; GO:0015574; F:trehalose transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd00212; PTS_IIB_glc; 1.
DR Gene3D; 3.30.1360.60; Glucose permease domain IIB; 1.
DR InterPro; IPR036878; Glu_permease_IIB.
DR InterPro; IPR018113; PTrfase_EIIB_Cys.
DR InterPro; IPR003352; PTS_EIIC.
DR InterPro; IPR013013; PTS_EIIC_1.
DR InterPro; IPR001996; PTS_IIB_1.
DR InterPro; IPR010973; PTS_IIBC_sucr.
DR InterPro; IPR011296; PTS_IIBC_treh.
DR NCBIfam; TIGR00826; EIIB_glc; 1.
DR NCBIfam; TIGR01996; PTS-II-BC-sucr; 1.
DR NCBIfam; TIGR01992; PTS-IIBC-Tre; 1.
DR PANTHER; PTHR30175; PHOSPHOTRANSFERASE SYSTEM TRANSPORT PROTEIN; 1.
DR PANTHER; PTHR30175:SF4; PTS SYSTEM TREHALOSE-SPECIFIC EIIBC COMPONENT; 1.
DR Pfam; PF00367; PTS_EIIB; 1.
DR Pfam; PF02378; PTS_EIIC; 1.
DR SUPFAM; SSF55604; Glucose permease domain IIB; 1.
DR PROSITE; PS51098; PTS_EIIB_TYPE_1; 1.
DR PROSITE; PS01035; PTS_EIIB_TYPE_1_CYS; 1.
DR PROSITE; PS51103; PTS_EIIC_TYPE_1; 1.
PE 4: Predicted;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Reference proteome {ECO:0000313|Proteomes:UP000193006};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT TRANSMEM 113..135
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 187..208
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 260..286
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 292..311
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 342..365
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 399..421
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 441..463
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 5..88
FT /note="PTS EIIB type-1"
FT /evidence="ECO:0000259|PROSITE:PS51098"
FT DOMAIN 108..469
FT /note="PTS EIIC type-1"
FT /evidence="ECO:0000259|PROSITE:PS51103"
FT ACT_SITE 27
FT /note="Phosphocysteine intermediate; for EIIB activity"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00421"
SQ SEQUENCE 469 AA; 49941 MW; 704C637B8D6472FE CRC64;
MSDLKQAAKE VLAAIGGNEN IASATHCVTR LRFALVDEGK VDKEALEKID VVRGSFSTNG
QFQVVIGQGT VDKVYKELVD LSGISESSKE ETKRAADKNL NPLQRAVKML ADIFIPILPA
IVTAGLLMGI NNILTAQGIF GEQAVVEMFP QWADIANIIN LIANTAFVFL PGLIGWSAVK
RFGGSELLGI VLGLMLVHPD LLNAWGYGAA QEAGEIPYWN LFGLDVAMVG YQGQVLPILV
ASFVLAKIEL ALRKRVPDGF QLLVVAPVAL LVTGFIAFVA IGPITFAIGN GITNLFVGIF
SNFAALGGLL YGAIYAPLVI TGMHHTFLAV DLQLIGSIGG TFLWPIVALS NIAQGSAALA
MYFILKDEKV KGLSLTSSIS AYLGITEPAM FGVNLRYRFP FICAIIGSAI AAIWITVNSV
IAPSIGVGGL PGIFSIEASG WFAFFIGMII AVTVPFVLTY LFARTRKTL
//
