ID   A0A1Y0H1U8_9BACT        Unreviewed;       351 AA.
AC   A0A1Y0H1U8;
DT   30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT   30-AUG-2017, sequence version 1.
DT   24-JAN-2024, entry version 20.
DE   SubName: Full=Endoglucanase {ECO:0000313|EMBL:ARU41586.1};
GN   ORFNames=CCB80_10720 {ECO:0000313|EMBL:ARU41586.1};
OS   Armatimonadetes bacterium Uphvl-Ar1.
OC   Bacteria; Armatimonadota.
OX   NCBI_TaxID=2004467 {ECO:0000313|EMBL:ARU41586.1, ECO:0000313|Proteomes:UP000196284};
RN   [1] {ECO:0000313|EMBL:ARU41586.1, ECO:0000313|Proteomes:UP000196284}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Uphvl-Ar1 {ECO:0000313|EMBL:ARU41586.1,
RC   ECO:0000313|Proteomes:UP000196284};
RA   Woodhouse J.N., Makower A.K., Ionescu D., Grossart H.-P., Neilan B.A.,
RA   Dittmann E.;
RT   "Draft genome sequences of three Uncultured Armatimonadetes, binned from a
RT   Microcystis sp. enrichment culture and from Microcystis bloom
RT   metagenomes.";
RL   Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000256|PIRSR:PIRSR001123-2};
CC       Note=Binds 2 divalent metal cations per subunit.
CC       {ECO:0000256|PIRSR:PIRSR001123-2};
CC   -!- SIMILARITY: Belongs to the peptidase M42 family.
CC       {ECO:0000256|ARBA:ARBA00006272, ECO:0000256|PIRNR:PIRNR001123}.
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DR   EMBL; CP021423; ARU41586.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1Y0H1U8; -.
DR   OrthoDB; 9772053at2; -.
DR   Proteomes; UP000196284; Chromosome.
DR   GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd05656; M42_Frv; 1.
DR   Gene3D; 2.40.30.40; Peptidase M42, domain 2; 1.
DR   Gene3D; 3.40.630.10; Zn peptidases; 1.
DR   InterPro; IPR008007; Peptidase_M42.
DR   InterPro; IPR023367; Peptidase_M42_dom2.
DR   PANTHER; PTHR32481; AMINOPEPTIDASE; 1.
DR   PANTHER; PTHR32481:SF0; AMINOPEPTIDASE YPDE-RELATED; 1.
DR   Pfam; PF05343; Peptidase_M42; 1.
DR   PIRSF; PIRSF001123; PepA_GA; 1.
DR   SUPFAM; SSF101821; Aminopeptidase/glucanase lid domain; 1.
DR   SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE   3: Inferred from homology;
KW   Aminopeptidase {ECO:0000256|ARBA:ARBA00022438};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR001123-2}; Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Reference proteome {ECO:0000313|Proteomes:UP000196284}.
FT   ACT_SITE        205
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-1"
FT   BINDING         63
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         175
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         175
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         206
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         228
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         316
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
SQ   SEQUENCE   351 AA;  38344 MW;  E8E6830114124957 CRC64;
     MNVELLKELT EAAGVPGQED LIRRIVYREL KDICDEITTD ALGNLICIKK GSGNGKKLML
     AAHMDEIGFV VKYIDDNGFL RIHPLGGFDP RMMAAQRVIV HAKSGPRNGL LMLGTKPKHM
     LSPTEANAGP KLEDYFVDTG LTKDEAKSQI RIGDMVTMNR DFQTMGDLYT CKSMDDRCAV
     FVMIEAVKAA KSHDVDVYAV ATTQEEIGLR GATAAGSGLK PNFCVALDVT LANDFPGIPA
     EYCVTKLGEG TAIKVLDSSL ICHPKMVEHF EDLAIKNNIK YQLELLTAGG TDAGGVQRQN
     GGVPSFTLSI PTRYIHTVNE TVHKDDVQAS IDLLARYIED CGSRDYAFKL D
//