ID A0A1Y0KPN7_9PSED Unreviewed; 360 AA.
AC A0A1Y0KPN7;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=Peptide chain release factor 1 {ECO:0000256|HAMAP-Rule:MF_00093};
DE Short=RF-1 {ECO:0000256|HAMAP-Rule:MF_00093};
GN Name=prfA {ECO:0000256|HAMAP-Rule:MF_00093};
GN ORFNames=B9K09_05635 {ECO:0000313|EMBL:ARU87479.1};
OS Pseudomonas sp. M30-35.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1981174 {ECO:0000313|EMBL:ARU87479.1, ECO:0000313|Proteomes:UP000196445};
RN [1] {ECO:0000313|EMBL:ARU87479.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M30-35 {ECO:0000313|EMBL:ARU87479.1};
RA He A., Zhao Q., Li H., Zhang J.;
RT "Complete genome sequence of the plant growth-promoting rhizobacterium
RT Pseudomonas rhizovicinus strain M30-35.";
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC translation in response to the peptide chain termination codons UAG and
CC UAA. {ECO:0000256|ARBA:ARBA00002986, ECO:0000256|HAMAP-Rule:MF_00093}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00093}.
CC -!- PTM: Methylated by PrmC. Methylation increases the termination
CC efficiency of RF1. {ECO:0000256|HAMAP-Rule:MF_00093}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000256|ARBA:ARBA00010835, ECO:0000256|HAMAP-
CC Rule:MF_00093}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP020892; ARU87479.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y0KPN7; -.
DR OrthoDB; 9806673at2; -.
DR Proteomes; UP000196445; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.160.20; -; 1.
DR Gene3D; 3.30.70.1660; -; 1.
DR Gene3D; 6.10.140.1950; -; 1.
DR HAMAP; MF_00093; Rel_fac_1; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004373; RF-1.
DR NCBIfam; TIGR00019; prfA; 1.
DR PANTHER; PTHR43804; LD18447P; 1.
DR PANTHER; PTHR43804:SF7; LD18447P; 1.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; Release factor; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00093};
KW Methylation {ECO:0000256|ARBA:ARBA00022481, ECO:0000256|HAMAP-
KW Rule:MF_00093}; Protein biosynthesis {ECO:0000256|HAMAP-Rule:MF_00093};
KW Reference proteome {ECO:0000313|Proteomes:UP000196445}.
FT DOMAIN 230..246
FT /note="Prokaryotic-type class I peptide chain release
FT factors"
FT /evidence="ECO:0000259|PROSITE:PS00745"
FT MOD_RES 237
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00093"
SQ SEQUENCE 360 AA; 40194 MW; 37028BAB077E4277 CRC64;
MKPSLLNKLD LMQDRFEELT ALLGDAEVIS NQSQFRAYSK EYAEIEPVNK TFSEFRKVLA
DLESAQALLK DSDPDMREMA EEEVAEAKEQ LVKLEGDLQR MLLPKDPNDD RNVFLEVRAG
TGGDEAAIFS GDLFRMYSRY AERRGWRVEI LSANEGEHGG YKEVIARIEG DNVYSKLKFE
SGAHRVQRVP ETESQGRVHT SACTVAVLPE PDEQAEININ PADLRVDTYR SSGAGGQHVN
TTDSAIRITH IPTGTVVECQ EERSQHKNRA KAMSWLAAKL KDQQDAAAHK EISDTRKLLV
GSGDRSERIR TYNFPQGRVT DHRINLTLYS LAEVMAGGVD AVIEPLLAEY QADQLAALGD
//
