ID A0A1Y0MI73_9FLAO Unreviewed; 238 AA.
AC A0A1Y0MI73;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=tRNA1(Val) (adenine(37)-N6)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_01872};
DE EC=2.1.1.223 {ECO:0000256|HAMAP-Rule:MF_01872};
DE AltName: Full=tRNA m6A37 methyltransferase {ECO:0000256|HAMAP-Rule:MF_01872};
GN ORFNames=BTO05_10490 {ECO:0000313|EMBL:ARV10042.1};
OS Winogradskyella sp. PC-19.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Winogradskyella.
OX NCBI_TaxID=754417 {ECO:0000313|EMBL:ARV10042.1, ECO:0000313|Proteomes:UP000195547};
RN [1] {ECO:0000313|EMBL:ARV10042.1, ECO:0000313|Proteomes:UP000195547}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PC-19 {ECO:0000313|EMBL:ARV10042.1,
RC ECO:0000313|Proteomes:UP000195547};
RA Kumagai Y., Yoshizawa S., Kogure K., Iwasaki W.;
RT "Trade-off between light-utilization and light-protection in marine
RT flavobacteria.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Specifically methylates the adenine in position 37 of
CC tRNA(1)(Val) (anticodon cmo5UAC). {ECO:0000256|HAMAP-Rule:MF_01872}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=adenosine(37) in tRNA1(Val) + S-adenosyl-L-methionine = H(+) +
CC N(6)-methyladenosine(37) in tRNA1(Val) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43160, Rhea:RHEA-COMP:10369, Rhea:RHEA-COMP:10370,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74411, ChEBI:CHEBI:74449; EC=2.1.1.223;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01872};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01872}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. tRNA
CC (adenine-N(6)-)-methyltransferase family. {ECO:0000256|HAMAP-
CC Rule:MF_01872}.
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DR EMBL; CP019332; ARV10042.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y0MI73; -.
DR OrthoDB; 5383291at2; -.
DR Proteomes; UP000195547; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0000179; F:rRNA (adenine-N6,N6-)-dimethyltransferase activity; IEA:InterPro.
DR GO; GO:0016430; F:tRNA (adenine-N6)-methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-UniRule.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR HAMAP; MF_01872; tRNA_methyltr_YfiC; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR020596; rRNA_Ade_Mease_Trfase_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR007848; Small_mtfrase_dom.
DR InterPro; IPR022882; tRNA_adenine-N6_MeTrfase.
DR PANTHER; PTHR47739; TRNA1(VAL) (ADENINE(37)-N6)-METHYLTRANSFERASE; 1.
DR PANTHER; PTHR47739:SF1; TRNA1(VAL) (ADENINE(37)-N6)-METHYLTRANSFERASE; 1.
DR Pfam; PF05175; MTS; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
DR PROSITE; PS01131; RRNA_A_DIMETH; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_01872};
KW Methyltransferase {ECO:0000256|HAMAP-Rule:MF_01872,
KW ECO:0000313|EMBL:ARV10042.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000195547};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691, ECO:0000256|HAMAP-
KW Rule:MF_01872};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_01872};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW Rule:MF_01872}.
FT DOMAIN 41..132
FT /note="Methyltransferase small"
FT /evidence="ECO:0000259|Pfam:PF05175"
SQ SEQUENCE 238 AA; 27318 MW; E460F89F5A21BAE1 CRC64;
MGIKPFKFKE FSINQDRSAM KIGTDGVLLG AWTSIRNQPF SILDIGAGTG ILSLMLAQRS
NAQVIEAIEI DDNAYEQCAD NFENSPWSDR LFCYHASLLE YTEEIEEQYD IIICNPPFYS
EDYKTEKISR DLARFQDAMP FEHLIFSVSQ FLAEGGVFSV VIPNKEEEKL VELASKVGLS
PKRILHVKGN PEAEVKRSLI EFSFNDEELK TSELIIENDR HNYTEDYINL TKDFYLKM
//
