ID A0A1Y1HSY4_KLENI Unreviewed; 715 AA.
AC A0A1Y1HSY4;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=KFL_000460220 {ECO:0000313|EMBL:GAQ80111.1};
OS Klebsormidium nitens (Green alga) (Ulothrix nitens).
OC Eukaryota; Viridiplantae; Streptophyta; Klebsormidiophyceae;
OC Klebsormidiales; Klebsormidiaceae; Klebsormidium.
OX NCBI_TaxID=105231 {ECO:0000313|EMBL:GAQ80111.1, ECO:0000313|Proteomes:UP000054558};
RN [1] {ECO:0000313|EMBL:GAQ80111.1, ECO:0000313|Proteomes:UP000054558}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIES-2285 {ECO:0000313|EMBL:GAQ80111.1,
RC ECO:0000313|Proteomes:UP000054558};
RX PubMed=24865297; DOI=10.1038/ncomms4978;
RA Hori K., Maruyama F., Fujisawa T., Togashi T., Yamamoto N., Seo M.,
RA Sato S., Yamada T., Mori H., Tajima N., Moriyama T., Ikeuchi M.,
RA Watanabe M., Wada H., Kobayashi K., Saito M., Masuda T.,
RA Sasaki-Sekimoto Y., Mashiguchi K., Awai K., Shimojima M., Masuda S.,
RA Iwai M., Nobusawa T., Narise T., Kondo S., Saito H., Sato R., Murakawa M.,
RA Ihara Y., Oshima-Yamada Y., Ohtaka K., Satoh M., Sonobe K., Ishii M.,
RA Ohtani R., Kanamori-Sato M., Honoki R., Miyazaki D., Mochizuki H.,
RA Umetsu J., Higashi K., Shibata D., Kamiya Y., Sato N., Nakamura Y.,
RA Tabata S., Ida S., Kurokawa K., Ohta H.;
RT "Klebsormidium flaccidum genome reveals primary factors for plant
RT terrestrial adaptation.";
RL Nat. Commun. 5:3978-3978(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
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DR EMBL; DF236995; GAQ80111.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y1HSY4; -.
DR STRING; 105231.A0A1Y1HSY4; -.
DR OrthoDB; 5480520at2759; -.
DR Proteomes; UP000054558; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 2.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR PANTHER; PTHR11254:SF446; HECT-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR Pfam; PF00632; HECT; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50237; HECT; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils}; Ligase {ECO:0000313|EMBL:GAQ80111.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000054558};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 396..715
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 1..199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 267..337
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 213..247
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 34..59
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 138..152
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 302..321
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 322..337
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 682
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 715 AA; 78113 MW; 285BC11A2D8AAF1A CRC64;
MEPNRKGAGG PRETSESIAE NAVHKLPMRL SRTMSDQARR KAEKDVRESE SKDKPAAKLT
SGKAQRVLKD VTNQRSAKHA TELTKKASQQ SRPVPIAAIG AGQAPNVHHA TTASAKEAEV
AVADTSEYKP ESASLLGRPQ EADSSQGPVE KGTTSVLPEG RSGLPFNTEE SPGIQVLEEG
AGANADTERK ADSKASMKSK ALPLPVAVPL SDTRREQRKL AAAMKVFEQL EQQRLQQQEM
ERKAQAKTLH VQQLLRKAMK GWKSIRSNAS LPANPSSLGV DEAGKLQAPG PSEQQTVNND
IKGKQKVEEI TSEEGQVNHR PRQTGKRNGS TSMPAPKVEV QSPSQAFGSA AVAIVDQSIQ
ELKEKRKKRV RTRGRQKQVE RVRMVESIIA LFSELKGEEL LDDFAVLFKG EEGMDAGGLT
AELFYLFLLS CLRAEGPGTD KKEWANTFLQ IGKVFIKSIL DQVPLPRRLA STFFKFVLDC
PLDLDDLGSF DPQLKRSYES ILAATSEDLD NMCLSFDSVG DPADAVTSEN RAQFVDQMVY
EKLLGCRLEA LEALREGMSQ IGPLYDQLRT LSPAALEDRM CGTEVMNGRD VAVFLHFPPG
ELSQAVHKEQ LLALLDAWDQ STLSDFLKFT TGLCVLPIGG LKNPDASPKH MIKVVSCTCA
SCKKRSGPAK SPCTLLPQAS TCFWTLRLPV CSIAEELEKK LLVAFKWGNG SFGQL
//
