ID A0A1Y1IMY4_KLENI Unreviewed; 820 AA.
AC A0A1Y1IMY4;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=KFL_009040040 {ECO:0000313|EMBL:GAQ92013.1};
OS Klebsormidium nitens (Green alga) (Ulothrix nitens).
OC Eukaryota; Viridiplantae; Streptophyta; Klebsormidiophyceae;
OC Klebsormidiales; Klebsormidiaceae; Klebsormidium.
OX NCBI_TaxID=105231 {ECO:0000313|EMBL:GAQ92013.1, ECO:0000313|Proteomes:UP000054558};
RN [1] {ECO:0000313|EMBL:GAQ92013.1, ECO:0000313|Proteomes:UP000054558}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIES-2285 {ECO:0000313|EMBL:GAQ92013.1,
RC ECO:0000313|Proteomes:UP000054558};
RX PubMed=24865297; DOI=10.1038/ncomms4978;
RA Hori K., Maruyama F., Fujisawa T., Togashi T., Yamamoto N., Seo M.,
RA Sato S., Yamada T., Mori H., Tajima N., Moriyama T., Ikeuchi M.,
RA Watanabe M., Wada H., Kobayashi K., Saito M., Masuda T.,
RA Sasaki-Sekimoto Y., Mashiguchi K., Awai K., Shimojima M., Masuda S.,
RA Iwai M., Nobusawa T., Narise T., Kondo S., Saito H., Sato R., Murakawa M.,
RA Ihara Y., Oshima-Yamada Y., Ohtaka K., Satoh M., Sonobe K., Ishii M.,
RA Ohtani R., Kanamori-Sato M., Honoki R., Miyazaki D., Mochizuki H.,
RA Umetsu J., Higashi K., Shibata D., Kamiya Y., Sato N., Nakamura Y.,
RA Tabata S., Ida S., Kurokawa K., Ohta H.;
RT "Klebsormidium flaccidum genome reveals primary factors for plant
RT terrestrial adaptation.";
RL Nat. Commun. 5:3978-3978(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
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DR EMBL; DF237853; GAQ92013.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y1IMY4; -.
DR STRING; 105231.A0A1Y1IMY4; -.
DR OrthoDB; 5475708at2759; -.
DR Proteomes; UP000054558; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0043138; F:3'-5' DNA helicase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000725; P:recombinational repair; IBA:GO_Central.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF13245; AAA_19; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000054558}.
FT DOMAIN 68..305
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT BINDING 89..96
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 820 AA; 92230 MW; E7A1F8E916259371 CRC64;
MSFFGLSSLS PPSEEGGTDR MVHIQTLVGG PKSKTAMNPR IPLLESFWKS LQGGFTLANL
HTIMESSYND PRQREIVVAP QNVHIKVLAC PGSGKTRVLV QRACHLIEHF GVKPSAIRVV
TFSRHAAADV GSRLQGVVKA QTIHSLCLEI AEAFEPILHN SLRYVPAACA WSTDSAEDLD
VSYLPDEKES VFQNAEAEVF FCDEHIYRLL RAFEAAGDEV DIAAPIRDLI PDYLLVDESQ
DLDATQFRVI ELLVKRFRTR IFMVGDKCQG IYKFRKSDSD LMDRVDTLGT YPSITFYLNN
NYRSTQTIVD FCNDMRPDRS MPPMATIRDF PSWLPRLCVF EERSEEIRFV ADLVQSYVTD
MGRVLGDIAI LSRTQREAYA LAHKFTEMGL PTKICTGDAV ESSHKHREGD PLTISSFHGA
KGLEFGVVIV TGCTDSFNKF LTPDELQQEA QLYYVACSRA KDILVITSGS RCISRMIFPV
KSSRFKVDMG DPKSASPIFG IFGDVNALYG SVIERVIYRQ ILSACLRESG SSDEVKMTDH
FANMCFLWTK AFEDDWRLRE PTEQDVRNLI RNKAAEFQLP ESHVAVHYEN RSLPSFARPS
KDDAHKRTAR FAAAVERIGK SYKRYVNFDH DTESTHALKT IADVAVCAHL AYPPAKTHYV
FSELHLGDLV KDKNLGLFSC TRRLVNGIMA SHFETRRAIG TLRHFAAGDV VTPELMLGTF
RVGLDIDLST KSLRGVADMV IGDTVYDIKC SSEKGGIQAA WVLQLMCYLS LARLNGLKVD
NIAIYCPLQG FVWKAPMHMW QRHEELIRRV NEEISGSSFR
//
