ID A0A1Y1IRP5_KLENI Unreviewed; 3979 AA.
AC A0A1Y1IRP5;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=KFL_007800030 {ECO:0000313|EMBL:GAQ91416.1};
OS Klebsormidium nitens (Green alga) (Ulothrix nitens).
OC Eukaryota; Viridiplantae; Streptophyta; Klebsormidiophyceae;
OC Klebsormidiales; Klebsormidiaceae; Klebsormidium.
OX NCBI_TaxID=105231 {ECO:0000313|EMBL:GAQ91416.1, ECO:0000313|Proteomes:UP000054558};
RN [1] {ECO:0000313|EMBL:GAQ91416.1, ECO:0000313|Proteomes:UP000054558}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIES-2285 {ECO:0000313|EMBL:GAQ91416.1,
RC ECO:0000313|Proteomes:UP000054558};
RX PubMed=24865297; DOI=10.1038/ncomms4978;
RA Hori K., Maruyama F., Fujisawa T., Togashi T., Yamamoto N., Seo M.,
RA Sato S., Yamada T., Mori H., Tajima N., Moriyama T., Ikeuchi M.,
RA Watanabe M., Wada H., Kobayashi K., Saito M., Masuda T.,
RA Sasaki-Sekimoto Y., Mashiguchi K., Awai K., Shimojima M., Masuda S.,
RA Iwai M., Nobusawa T., Narise T., Kondo S., Saito H., Sato R., Murakawa M.,
RA Ihara Y., Oshima-Yamada Y., Ohtaka K., Satoh M., Sonobe K., Ishii M.,
RA Ohtani R., Kanamori-Sato M., Honoki R., Miyazaki D., Mochizuki H.,
RA Umetsu J., Higashi K., Shibata D., Kamiya Y., Sato N., Nakamura Y.,
RA Tabata S., Ida S., Kurokawa K., Ohta H.;
RT "Klebsormidium flaccidum genome reveals primary factors for plant
RT terrestrial adaptation.";
RL Nat. Commun. 5:3978-3978(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the UPL family. TOM1/PTR1 subfamily.
CC {ECO:0000256|ARBA:ARBA00034494}.
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DR EMBL; DF237729; GAQ91416.1; -; Genomic_DNA.
DR STRING; 105231.A0A1Y1IRP5; -.
DR OrthoDB; 1214833at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000054558; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR CDD; cd00078; HECTc; 1.
DR CDD; cd14327; UBA_atUPL1_2_like; 1.
DR Gene3D; 6.10.250.1630; -; 1.
DR Gene3D; 1.10.8.10; DNA helicase RuvA subunit, C-terminal domain; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR010309; E3_Ub_ligase_DUF908.
DR InterPro; IPR010314; E3_Ub_ligase_DUF913.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR025527; HUWE1/Rev1_UBM.
DR InterPro; IPR015940; UBA.
DR InterPro; IPR009060; UBA-like_sf.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR PANTHER; PTHR11254:SF446; HECT-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR Pfam; PF06012; DUF908; 2.
DR Pfam; PF06025; DUF913; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF00627; UBA; 1.
DR Pfam; PF14377; UBM; 3.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00165; UBA; 1.
DR SUPFAM; SSF48371; ARM repeat; 2.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF46934; UBA-like; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS50030; UBA; 1.
PE 3: Inferred from homology;
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000054558};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 1304..1345
FT /note="UBA"
FT /evidence="ECO:0000259|PROSITE:PS50030"
FT DOMAIN 3637..3979
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 683..728
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1012..1036
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1556..1591
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1758..1808
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1827..1866
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2088..2338
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2545..2757
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2772..2835
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3168..3237
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3476..3550
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1018..1032
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1559..1579
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1758..1781
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1849..1866
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2146..2198
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2232..2277
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2285..2304
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2320..2338
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2547..2571
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2685..2709
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2793..2810
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3168..3198
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3489..3550
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 3946
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 3979 AA; 428957 MW; 8632D930EEADA300 CRC64;
MKTKKRKAAE VPHAVKACTN EVTSAPPESL TDVLRNFKWD YDKGDLNHWV DLLNFFDAYF
EKHLPESVKL QLDGTLKQEQ HPVSKDTLLQ ILRVTAVILE NSYNKQLYGS VEHLTLLLGH
EDTDVVIATL KALLALVRKP MTSGRSSRSY SNPALNVRLL SLCQGWGGKE EGLGLLASIT
EEVQDQKALQ LGSTLFFDFY AEAKDEIASA TGQKSIRLPE VHTRPESDSL LLKDLADHYK
VPPELRFSLL SRIRAARSFA SLEGRRTYMA IRLLAFTILV QSSPDYEELS TFFANEPEFA
TDLVALVKAE GRVPDEIRTL AVQALTAQAQ DRPRQSNILA IITAGGHQGV LPTVMQRSVG
LLSKGDSRAN AAFVEALLTL LTNLATSTSG CTALREAGLV STLLPLIKDV DPEHIVIVSL
ATRVLDAYMD YSNSASAMFR ELHGVEDAAE RLTREIDLHV QKAEGAPQDP VVPRYPLRQL
IKSLMRMVAL ANYSNQQGTA RVQNLEESRL PYVLQTVYDK AALFGPEIFA LGSTILAELI
NHDPMCYPAL HAARVPHAFL DVLEKGILPT ADCLMTVPSA LVAICLNAAG LELVKQRKVL
NCLVPIMTSP QYTRAMQGET PTVLGAGLDE LMRHVPTLRQ DGVAVVLKLL RKVGAMGGSK
TALEQLRGTV KAERAAAALL ARTTAEPAPE ATRGQESVEP AVAEMTESGD GPEPMDTGDS
EAPPPVPEAP PAVVGEILVE DKELIGELSD AELEKFLPDC ITNVMRMLET VLSNQETCRA
FIEKRGVEAL LELYTLPQMP VTFGAQSGAA HSMTVAFRAF APQQSHSLVR AVVSVLREQL
KRGAAATQKQ LAGKKLSDLD KDVRAEVVRH WASAECLLSL ASSMVRSFPG VMGEMCGGAA
DVLKDLDVLY KEAVWQGAFL LDESRRAGQN AKEVTPPAQE DSSMVQEYVD QYGNRLGITD
VRWIPGRSAR ARLIRGRARA AAENGAMEAV DHAVDDPEQA LADLDDALAA QDDPETPMAS
DTGDNSATPN AAQAAKAAEK AEKAMYEVVG RLATAGRGMY VALGKSIVQS SRRGQDVTTL
NSQAKKYIAW LAESAKSAFS LSAQAKDAPL AVKCAFVSRI ADDLLATVVD VRRRTCNTAI
LGGLDALGAI QEVLLTFEAS AQLLWAQLAK AGEQGDKGAA AGELSHGLFD LLLSLTRFVE
QIVNAQLLLA PTSSLQLMVQ PVQGAVKPAA KDAESFVLGL QQDVLDVVLP IWGHPLFAKS
PAPFIESIFT IMTQIYSGVG DKKGQKISAG GVPAIPAPRP AALPLDEDAI NMIVEMGFSR
QRAEDALRHV RTNDVEVALE WLFSHPEEPP AASTATAAGG SDEDELARAL ALSMGGIDTD
VTDAGVPEKP EEEPPLEVPA VKDTLSMAVH LLVQAGEVAF SMTDLLVCLC NRKEGQDRKE
VVAYLVEQLK VAKESGDEGS LSTVAHVLAL VMHEESNTRV HAAQAGLVDI VLELLAGFVE
RVKDADVFTT AAAGEAPKQA VHPPWVDALF LVLDGFSTNK MQLGEELDVG LNAVRGVPPA
QNANPTQAAN QTPNPATGAA ETAQEKKEEK TKESTMLSAL CNAGGLLSGK QQAQAMTICC
ALLQKQLPLD AAQAVLQLTS HLSKSHKLAV QFLDSGALSA VLARPEVYMN PRFEPLVASI
IRHVLEDPHT LQQAMEAEIK QALVTTLARQ MGTLSPRMFL TTMAPVICRE PSVFMVAAAA
VCQVEKVNGR QTIVLKETKE KTDKGEPKAK DEKKDASSTK PEEPGASDAA PKATPSKAPR
GSHPGKKISH SFTAVINQLL EVVQRYSPPQ SLEKSAPKKT AGADEASPMD VDVDDKGKAK
VEETAAGKEK DEEVGLSAAQ RAEAARVLFV LRLLTEFCLL YSSCLSVVLR RDWESAQGKG
TGGLLHHILH QLVTYRALGA EARAALDSWD LLKAIPPTAS SFLNAICLRS SEGRKRLVTD
MAKALLSIPD RGAAPATGEA VPEEGKVDTL IGLALNLLSA GPTASAAGPS FSAETLKTTV
EAGMVQSLTH VLTNVDLDHP RAPTLVSKIL KALEALTRVS PSIAAHAPAS RLGHVRGDTS
ARTEVVGSQG EAPPATAGGE TGAGEGRPAQ GAEVGGAPTG SQPAAEEERP PHDEEQDRMD
HDRLNRHLQR MAVNRMAERD RFREITEDIN RMVEDIRDAE EDEASDDDEE HPGPMRMMAG
FGGPVPFGHE DEGPSDDEEP SEPDEDDDDE GDDDDNDDDN DGEPDDGEED DQDGDEHLEG
DPGPLTDEEG DEEEEEEEDE DEDDGVRYAL AHPPPAVHDA DEDEEMDMMD EDEEEDEDHD
EWHDDHITEV HWGGGPRHTG GQQVVAHGHQ MHLLDMARGG GAMASVGNLF AGLRRQVEGR
GNRAITYRPY PERSARAEAR IGPQQHPLLS RGPTAANPTP PMATSTWRNV ASHHVIVGPS
GAGDPEYARL LAVDAALMAN SGVDGWPAGG GAGGFVDFGE GMASGGARRG VRTDRMASWA
EDSQMQGGGL NATIAELVEG DFVSQLTVPE PPPPEPERPA PAVPTSQPEE PKPDEPQAME
ESGQEGAPAG GSADRANEPP AAEATSEMAV EQEPAARAAP VAPPDEGMGA LDDQPGASTG
EPAEQGDPPA EEHGDVQMGE GGQGRDAPDA SDGGSNMGES LRSLDVEIGS RDGNEDVHAG
EREHGAPAGH TEPPADPSQQ AEETARPEGV GPTVAAPVEA APETATGMSG IDPTFLDALP
PELRAEVLEN EQAAARRQAR QATLAALAQP PAAPAAPADP TNPPTNEAQN PDPAPSAAQV
PEAAPGPSSN NPIEALLTND VDPEFLAALP PDMQAEVLAQ QRVAQAAARH AASATSADMD
SASIIATFPP DLREEVLLTA SEQVLAALPP ALLAEAQLLR ERHLAHYRHA DPGRAAQRTR
LHQINHALQA RVRGNGHVST HPHHVPEPHR KEVEGRPLLE TKELGSILRL LWLAQPLGKG
LLPRLLLNLS LHSKTRTAML RQLLTIMRPK DEKASADIEA MPDRLFGAQS NVVYARSDAT
TDGFPPLLSR RVLDTLTYLA RHSGTIAHQL LQLEVDAPEA PTDRKGKAKV VESEEVAKTV
TEIPLVLLLR LLSQPLYVRS NSHLEQLLAL TEVVMRHAEA DMAEHRARVR AHEKELERQK
AKAEAEQKEK EKKAAEDAAA RAAAPAPTEG DVSTEAAPAD GPPAAAQETS AQPERMDGLP
ELWDVDRQEK DEGEKLEAAP IGPCEAETVL KELPAKELSY LCRLLAHQGF PEATYPKVST
ILKLLANPVP AHRTLFLGEL KTDAHTLVRP AVAELETLSH SAAVSAAMDA SAGAALLRVL
LMIGYLMAGK RVKDGVTKTE SAGAEGVAVI DELAPALEPV WQALSGCVSQ LETQLVQTTA
LGGLNVAAGG VAAAVQSLPP SVGKVLPHIE AFLMWCEKLQ EAGELKKASE KAKKASEAAE
KASESSEKAS EKASGSGASG SQDTTMREVS ESRPNSPGSR QEGGESPAHS TGTSTPRLWS
QQSTPRDVAR PSTFQAFAEK HRKLLNAFIR QNPNLLEGTF NLILKTPRLI DFDNKRNFFR
SKIRQQHEQT HHHHSLRVPV RRAYVLEDSY NQLRMRRPEE LRGRLVVHFQ GEEGIDAGGL
TREWYQLLSR VIFDKGTLLF TSAAGNDSTY QPNPNSGYQT EHLSYFKFVG RVVGKALYDG
QLLDVYFTRS FYKHILGNKI TYHDIEAIDP EYYKNLKWML DNDITGVLDL TFAVEADEEQ
HILYEKQQLH DHELKPGGRD IPVTEANKRE YVNLVAEHRM TTAIRPQISA FLDGFHELIP
KDLIAVFNDK ELELLISGLP EIDIEDLKQN TEYTGFTGAS PVVQWFWDVV RNFSKEDQAR
LLQFTTGTSK VPLEGFKALQ GISGPQRFQI HKAYGAPDRL PSAHTCFNQL DLPEYQMREQ
LQERLLLAIH EGSEGFGFG
//
