ID A0A1Y1IUU3_KLENI Unreviewed; 1024 AA.
AC A0A1Y1IUU3;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=mRNA (guanine-N(7))-methyltransferase {ECO:0000256|ARBA:ARBA00011926};
DE EC=2.1.1.56 {ECO:0000256|ARBA:ARBA00011926};
GN ORFNames=KFL_010170055 {ECO:0000313|EMBL:GAQ92457.1};
OS Klebsormidium nitens (Green alga) (Ulothrix nitens).
OC Eukaryota; Viridiplantae; Streptophyta; Klebsormidiophyceae;
OC Klebsormidiales; Klebsormidiaceae; Klebsormidium.
OX NCBI_TaxID=105231 {ECO:0000313|EMBL:GAQ92457.1, ECO:0000313|Proteomes:UP000054558};
RN [1] {ECO:0000313|EMBL:GAQ92457.1, ECO:0000313|Proteomes:UP000054558}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIES-2285 {ECO:0000313|EMBL:GAQ92457.1,
RC ECO:0000313|Proteomes:UP000054558};
RX PubMed=24865297; DOI=10.1038/ncomms4978;
RA Hori K., Maruyama F., Fujisawa T., Togashi T., Yamamoto N., Seo M.,
RA Sato S., Yamada T., Mori H., Tajima N., Moriyama T., Ikeuchi M.,
RA Watanabe M., Wada H., Kobayashi K., Saito M., Masuda T.,
RA Sasaki-Sekimoto Y., Mashiguchi K., Awai K., Shimojima M., Masuda S.,
RA Iwai M., Nobusawa T., Narise T., Kondo S., Saito H., Sato R., Murakawa M.,
RA Ihara Y., Oshima-Yamada Y., Ohtaka K., Satoh M., Sonobe K., Ishii M.,
RA Ohtani R., Kanamori-Sato M., Honoki R., Miyazaki D., Mochizuki H.,
RA Umetsu J., Higashi K., Shibata D., Kamiya Y., Sato N., Nakamura Y.,
RA Tabata S., Ida S., Kurokawa K., Ohta H.;
RT "Klebsormidium flaccidum genome reveals primary factors for plant
RT terrestrial adaptation.";
RL Nat. Commun. 5:3978-3978(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 5'-end (5'-triphosphoguanosine)-(ribonucleoside) in mRNA +
CC S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-
CC triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:67008, Rhea:RHEA-COMP:17166, Rhea:RHEA-
CC COMP:17167, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:156461,
CC ChEBI:CHEBI:167617; EC=2.1.1.56;
CC Evidence={ECO:0000256|ARBA:ARBA00024288};
CC -!- SIMILARITY: In the N-terminal section; belongs to the dsDNA virus mRNA
CC guanylyltransferase family. {ECO:0000256|ARBA:ARBA00008556}.
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DR EMBL; DF237966; GAQ92457.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y1IUU3; -.
DR STRING; 105231.A0A1Y1IUU3; -.
DR Proteomes; UP000054558; Unassembled WGS sequence.
DR GO; GO:0004482; F:mRNA 5'-cap (guanine-N7-)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR004971; mRNA_G-N7_MeTrfase_dom.
DR InterPro; IPR039753; RG7MT1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR12189:SF2; MRNA CAP GUANINE-N7 METHYLTRANSFERASE; 1.
DR PANTHER; PTHR12189; MRNA GUANINE-7- METHYLTRANSFERASE; 1.
DR Pfam; PF03291; mRNA_G-N7_MeTrfase; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000054558};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 4..170
FT /note="MRNA cap 0 methyltransferase"
FT /evidence="ECO:0000259|Pfam:PF03291"
FT REGION 444..472
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 885..907
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 353..429
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 696..756
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 987..1021
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1024 AA; 116998 MW; 3643361A28E887C7 CRC64;
MEGLRAFHNV VKETLLSEAL SRCRNGRDLK VLDLACGCLG DVHKYDRTGR IRRVVGLDVD
GKALEEAERR SRPYAGRMDV SLLPPIDCRD LDLASEALGG ERFDLLVCNF AVQYFTTGPK
ETARFACFIK GHLDGPRSIF IGCAMNGDKV DLISDASGTF ANDIIGVQRE GETFAVAMNE
RTRYYAKQGA SISEPIFRPE SLITGALESG LRLLDWRSFA EYDVAQRALA EIHGDGLEAA
ARRYDIHVVE EFLQHLEEAL EGRALNGLED EHKAPAILGL GVKVRPGEGD VKELVVIGAN
ISSIGAFFSV EGPPRVLPPC LFFSHWKAAL PPLIKRQRGE GPSVVAPIQG GLVEAHAQAT
ANYESQIEKL QARIDGYERI QNQTDNELRR ERASREIVNS QLTESKRHIK DLEKDIERIR
REGRKTEDKL QNELDYEKRS RKVQMDKLEK ERSAEMKKSL AEQAEKHRKR EEELRKLREE
EKKRYEAKVD ELRKERDEAL ARQKELSEAA EAKRAIEIED LEGRRRRAEE EVKKLKEELQ
KKEHEREEAI AGWSHVRKGL TLANQQLNRE NADLQTAREE AIALMDRLRT VYKNLPRRAF
DLPRRVETQE ELTDVLESLI KDLNSDRSRQ SNQLDDIFRR VTRAVSNFRG FCERAENRVN
IADSNDIQTM IGNLENSLTH AQNMHEGARE TILGLERTAE TRKRDLERAL RDLDDLLSGT
VRADASEQVK TIKDRLDAAR EEARAFGKAA ARAEELRRAK AAEAERTGAQ LASLTKEVRR
LNAVVAHGNE REESMREKIR DFEKLCRDVL VSQEHREQLL NHADPKLREV GSIVKTLLDS
KETLERALQE SRERESARME LDDEIEELKT SKRALERQNA ELMAKASDLR DEVQRSNAEH
EHEKSRITAD YQAREAAWQR SAQNVSRDRE TWMQGAKSEF EQIYAERVRE LDGFRAQAAA
EVDAHRADRV ALEGRVRIAE QKAIEDEQNA EQRIAEIGAQ VRQIDKNYTR ASEEKQRELE
ELNV
//
