ID A0A1Y1YXA1_9FUNG Unreviewed; 1015 AA.
AC A0A1Y1YXA1;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Glycine cleavage system P protein {ECO:0000256|RuleBase:RU364056};
DE EC=1.4.4.2 {ECO:0000256|RuleBase:RU364056};
GN ORFNames=K493DRAFT_312043 {ECO:0000313|EMBL:ORY02504.1};
OS Basidiobolus meristosporus CBS 931.73.
OC Eukaryota; Fungi; Fungi incertae sedis; Zoopagomycota;
OC Entomophthoromycotina; Basidiobolomycetes; Basidiobolales; Basidiobolaceae;
OC Basidiobolus.
OX NCBI_TaxID=1314790 {ECO:0000313|EMBL:ORY02504.1, ECO:0000313|Proteomes:UP000193498};
RN [1] {ECO:0000313|EMBL:ORY02504.1, ECO:0000313|Proteomes:UP000193498}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 931.73 {ECO:0000313|EMBL:ORY02504.1,
RC ECO:0000313|Proteomes:UP000193498};
RG DOE Joint Genome Institute;
RA Mondo S.J., Dannebaum R.O., Kuo R.C., Labutti K., Haridas S., Kuo A.,
RA Salamov A., Ahrendt S.R., Lipzen A., Sullivan W., Andreopoulos W.B.,
RA Clum A., Lindquist E., Daum C., Ramamoorthy G.K., Gryganskyi A., Culley D.,
RA Magnuson J.K., James T.Y., O'Malley M.A., Stajich J.E., Spatafora J.W.,
RA Visel A., Grigoriev I.V.;
RT "Pervasive Adenine N6-methylation of Active Genes in Fungi.";
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The glycine cleavage system catalyzes the degradation of
CC glycine. {ECO:0000256|RuleBase:RU364056}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glycine + H(+) + N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage
CC complex H protein] = CO2 + N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-
CC L-lysyl-[glycine-cleavage complex H protein]; Xref=Rhea:RHEA:24304,
CC Rhea:RHEA-COMP:10494, Rhea:RHEA-COMP:10495, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:57305, ChEBI:CHEBI:83099,
CC ChEBI:CHEBI:83143; EC=1.4.4.2;
CC Evidence={ECO:0000256|ARBA:ARBA00043839,
CC ECO:0000256|RuleBase:RU364056};
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000256|ARBA:ARBA00001933,
CC ECO:0000256|PIRSR:PIRSR603437-50, ECO:0000256|RuleBase:RU364056};
CC -!- SUBUNIT: The glycine cleavage system is composed of four proteins: P,
CC T, L and H. {ECO:0000256|RuleBase:RU364056}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000256|RuleBase:RU364056}.
CC -!- SIMILARITY: Belongs to the GcvP family. {ECO:0000256|ARBA:ARBA00010756,
CC ECO:0000256|RuleBase:RU364056}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ORY02504.1}.
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DR EMBL; MCFE01000056; ORY02504.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y1YXA1; -.
DR STRING; 1314790.A0A1Y1YXA1; -.
DR InParanoid; A0A1Y1YXA1; -.
DR OrthoDB; 177349at2759; -.
DR Proteomes; UP000193498; Unassembled WGS sequence.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0004375; F:glycine dehydrogenase (decarboxylating) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006546; P:glycine catabolic process; IEA:InterPro.
DR CDD; cd00613; GDC-P; 2.
DR Gene3D; 3.90.1150.10; Aspartate Aminotransferase, domain 1; 2.
DR Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 2.
DR HAMAP; MF_00711; GcvP; 1.
DR InterPro; IPR003437; GcvP.
DR InterPro; IPR049316; GDC-P_C.
DR InterPro; IPR049315; GDC-P_N.
DR InterPro; IPR020581; GDC_P.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR NCBIfam; TIGR00461; gcvP; 1.
DR PANTHER; PTHR11773:SF1; GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL; 1.
DR PANTHER; PTHR11773; GLYCINE DEHYDROGENASE, DECARBOXYLATING; 1.
DR Pfam; PF21478; GcvP2_C; 1.
DR Pfam; PF02347; GDC-P; 2.
DR SUPFAM; SSF53383; PLP-dependent transferases; 2.
PE 3: Inferred from homology;
KW Mitochondrion {ECO:0000256|RuleBase:RU364056};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU364056};
KW Pyridoxal phosphate {ECO:0000256|PIRSR:PIRSR603437-50,
KW ECO:0000256|RuleBase:RU364056};
KW Reference proteome {ECO:0000313|Proteomes:UP000193498};
KW Transit peptide {ECO:0000256|RuleBase:RU364056}.
FT DOMAIN 65..492
FT /note="Glycine cleavage system P-protein N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02347"
FT DOMAIN 677..793
FT /note="Glycine cleavage system P-protein N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02347"
FT DOMAIN 836..957
FT /note="Glycine dehydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF21478"
FT MOD_RES 764
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000256|PIRSR:PIRSR603437-50"
SQ SEQUENCE 1015 AA; 111022 MW; 822C5E39BE200F8C CRC64;
MACSVRVAAR LLTSRTTVTL KSFATPLVVR ALPKPSVAPR FATRFLHTAK PPAKDVFAPL
DSFPRRHNGS GEKAVDSMLK TIGVKSVDDL IAKTVPSSIR AAKALALDNG YPERELLKRL
KDIASKNKVY RSYIGMGYTD TVVPNVILRN VMENPAWYTQ YTPYQAEISQ GRLESLLNYQ
TMVADLTGME IANASLLDEG TAAGEAMVMC YTASRNKKKT FFVDENCHPQ TIEVLKTRAE
GFGINVVVGN YKTYDFEGAQ KDLSGVLIQY PANDGIVIDY QGFTDRIHQL GGQVVCATDL
LALTMLKPPG EFGADIVLGN SQRFGVPLGY GGPHAAFFAC TKDNARRMPG RIVGVSKDAA
GKKALRLALQ TREQHIRREK ATSNICTAQA LLANMAAMYA VYHGPEGIKQ IAQRIHNLTT
ILSEGIRKAG HTIQNDTFFD TLTINVTGGS ESVIKKAADN GINLRHIDES TVGITLDETV
TRKDLEDLLK VFSKDGSAPD VSVLASELGV SESKPAQSFP SNLARTSKYL EHQVFHSYHS
ETEMLRYIHH LQSKDLSLTH SMIPLGSCTM KLNATTEMVP VTWPEFGNIH PFAPADQVEG
YKIMLDELAQ DLAVVTGFDE VSLQPNSGAQ GEYAGLRTIK AFHESRGEGQ RNVCLIPISA
HGTNPASAAM AGMKVVIVNC KPNGNLDLDD LKAKAEKHKD QLSAVMVTYP STFGVFEDGI
KDLCDIIHNN GGQVYMDGAN MNAQIGLCSP GEIGADVCHL NLHKTFCIPH GGGGPGMGPI
GVKAHLAPFL PGHPLVKTGG EKAIGPISAA PFGSASILPI SWAYIKMMGG KGITEATQQA
ILNANYMRSR LQDHYKVLYT NENGMCAHEF ILDIRPFGTT AGVEAIDVAK RLQDYGFHSP
TMSWPVANTL MIEPTESESK AELDRFCDAM ISIREEIREI EEGKQPKQQN ILKNSPHTLD
RLMADEWKQP YSREQAAYPK GWLRERKFWP SVSRIDDAYG DRNLVCSCPP ISDYQ
//
