ID A0A1Y2AIE9_9FUNG Unreviewed; 1135 AA.
AC A0A1Y2AIE9;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 22-FEB-2023, entry version 19.
DE RecName: Full=Phosphodiesterase {ECO:0000256|RuleBase:RU363067};
DE EC=3.1.4.- {ECO:0000256|RuleBase:RU363067};
GN ORFNames=LY90DRAFT_675945 {ECO:0000313|EMBL:ORY22296.1};
OS Neocallimastix californiae.
OC Eukaryota; Fungi; Fungi incertae sedis; Chytridiomycota;
OC Chytridiomycota incertae sedis; Neocallimastigomycetes; Neocallimastigales;
OC Neocallimastigaceae; Neocallimastix.
OX NCBI_TaxID=1754190 {ECO:0000313|EMBL:ORY22296.1, ECO:0000313|Proteomes:UP000193920};
RN [1] {ECO:0000313|EMBL:ORY22296.1, ECO:0000313|Proteomes:UP000193920}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=G1 {ECO:0000313|EMBL:ORY22296.1,
RC ECO:0000313|Proteomes:UP000193920};
RG DOE Joint Genome Institute;
RA Haitjema C.H., Gilmore S.P., Henske J.K., Solomon K.V., De Groot R.,
RA Kuo A., Mondo S.J., Salamov A.A., Labutti K., Zhao Z., Chiniquy J.,
RA Barry K., Brewer H.M., Purvine S.O., Wright A.T., Boxma B., Van Alen T.,
RA Hackstein J.H., Baker S.E., Grigoriev I.V., O'Malley M.A.;
RT "A Parts List for Fungal Cellulosomes Revealed by Comparative Genomics.";
RL Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000256|RuleBase:RU363067};
CC Note=Binds 2 divalent metal cations per subunit. Site 1 may
CC preferentially bind zinc ions, while site 2 has a preference for
CC magnesium and/or manganese ions. {ECO:0000256|RuleBase:RU363067};
CC -!- SIMILARITY: Belongs to the cyclic nucleotide phosphodiesterase family.
CC {ECO:0000256|RuleBase:RU363067}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ORY22296.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MCOG01000250; ORY22296.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y2AIE9; -.
DR STRING; 1754190.A0A1Y2AIE9; -.
DR OrthoDB; 5479253at2759; -.
DR Proteomes; UP000193920; Unassembled WGS sequence.
DR GO; GO:0004114; F:3',5'-cyclic-nucleotide phosphodiesterase activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR CDD; cd00077; HDc; 1.
DR Gene3D; 3.30.450.40; -; 2.
DR Gene3D; 1.10.1300.10; 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain; 1.
DR InterPro; IPR003018; GAF.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR InterPro; IPR003607; HD/PDEase_dom.
DR InterPro; IPR023088; PDEase.
DR InterPro; IPR002073; PDEase_catalytic_dom.
DR InterPro; IPR036971; PDEase_catalytic_dom_sf.
DR InterPro; IPR023174; PDEase_CS.
DR PANTHER; PTHR11347; CYCLIC NUCLEOTIDE PHOSPHODIESTERASE; 1.
DR PANTHER; PTHR11347:SF221; DUAL 3',5'-CYCLIC-AMP AND -GMP PHOSPHODIESTERASE 11; 1.
DR Pfam; PF01590; GAF; 1.
DR Pfam; PF00233; PDEase_I; 1.
DR PRINTS; PR00387; PDIESTERASE1.
DR SMART; SM00065; GAF; 1.
DR SUPFAM; SSF55781; GAF domain-like; 2.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR PROSITE; PS00126; PDEASE_I_1; 1.
DR PROSITE; PS51845; PDEASE_I_2; 1.
PE 3: Inferred from homology;
KW cGMP {ECO:0000256|ARBA:ARBA00022535};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU363067};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR623088-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000193920}.
FT DOMAIN 789..1123
FT /note="PDEase"
FT /evidence="ECO:0000259|PROSITE:PS51845"
FT REGION 465..484
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 869
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR623088-1"
FT BINDING 869..873
FT /ligand="AMP"
FT /ligand_id="ChEBI:CHEBI:456215"
FT /evidence="ECO:0000256|PIRSR:PIRSR623088-2"
FT BINDING 873
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR623088-3"
FT BINDING 910
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR623088-3"
FT BINDING 911
FT /ligand="AMP"
FT /ligand_id="ChEBI:CHEBI:456215"
FT /evidence="ECO:0000256|PIRSR:PIRSR623088-2"
FT BINDING 911
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR623088-3"
FT BINDING 911
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR623088-3"
FT BINDING 1026
FT /ligand="AMP"
FT /ligand_id="ChEBI:CHEBI:456215"
FT /evidence="ECO:0000256|PIRSR:PIRSR623088-2"
FT BINDING 1026
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR623088-3"
FT BINDING 1080
FT /ligand="AMP"
FT /ligand_id="ChEBI:CHEBI:456215"
FT /evidence="ECO:0000256|PIRSR:PIRSR623088-2"
SQ SEQUENCE 1135 AA; 131528 MW; C88F6E39E048E49E CRC64;
MSSHSLHKKK NRIGVNENIG LKKRHFYPLN HNELSKEFEG KVYSSPNLSF KFNRSNEIHY
QLNKLSKKLS HTIFDEDESF SVLNDSYTQI NSSLERIVSK MLNIANAEAA SLSLIKPIET
SSLNNFSTSN LSLSNNIYGE VNSKIDHPIN KAFKLELTTF INRNEHNKKE RRKSENKTYK
MYRNVKVKKI EKDISVDLSE IQYFCCEVVQ RNKVIIENNS EIINKRSWLD LNGNVRSIEN
NNKRYISSIN LFQDLDPNNY DEKNSLFKSG YSIENVENGE SSYEDPELTY KKKYSYKSDY
SLDNLKEIES GTDSHIRNFI GIPISCCVDR KLVPWMQNKC YNNNVIGVVC LYNKYNPIHN
IDNCINDSNE NMSSLNTLSD SNSSINKKKG NIKVFTNNDY EKLKVYLKLL GSAIQNNYLY
SSYNLCKNEI NNRYKKFIQY EKEIEEEVKD DSDLVSIIDN MGKETTKIND SDETNEQPKN
SITKDEILDD DGKAIMNGDS IECLIQKTQK NVTSSILKNE IENKLIDLKK ENLYYREAQK
ELIEKMKEMS NKNDVLTDQV SRHSIILQLV NILQSLFGEL DYYTLLRKIV SSAQELVGSD
QASLYMVQDN DTFRYVNFDS EDDNKYEDIP ISKGGLIGHV IQTGELLNIG DAYLDKNYNG
TIDTDHKYRT KSIIVVPIFR STQIVNGGNE TNFFSNYNGG FYIPDPTEND KEDAKKVISV
LKLVNKLGED GEVIEFSDYD VMVIQFFAAY CAISMERAIL LERNQKQKML MDVNVEMIAY
HMTTSVEDVD HFYEKYLKDE SKTFINPEIY QNWDFDTHSL KDQNEELILT IYSMFQEFNF
TETFQIPEKM LVHFLMTVHK NYRTNVQYHN FSHASAVTHT LYLLLKFGGL RELINDQFDI
LVMLVSCICH DIDHRGTNNQ FQVSAKTMLA VESSDSTMER HHFNHTINIL SLPDHNIFKN
LSKENYKRAL NIMESCIIST DLSIFFKNLK SINELGDRYI NGENLDLENN KRNLELLHGI
VMTSCDLGSM FKPFEETRLI AEDVLIESFE QGKKEKELGL QFTSALVDRA STEKEIPKVQ
YEFFSNVIEP AAKALVKFLP KAKYILEGVQ RNKECWRQLM EKKIEYAIGS YTHTS
//
