ID A0A1Y2APG8_9FUNG Unreviewed; 780 AA.
AC A0A1Y2APG8;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 22-FEB-2023, entry version 18.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=LY90DRAFT_389665 {ECO:0000313|EMBL:ORY23845.1};
OS Neocallimastix californiae.
OC Eukaryota; Fungi; Fungi incertae sedis; Chytridiomycota;
OC Chytridiomycota incertae sedis; Neocallimastigomycetes; Neocallimastigales;
OC Neocallimastigaceae; Neocallimastix.
OX NCBI_TaxID=1754190 {ECO:0000313|EMBL:ORY23845.1, ECO:0000313|Proteomes:UP000193920};
RN [1] {ECO:0000313|EMBL:ORY23845.1, ECO:0000313|Proteomes:UP000193920}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=G1 {ECO:0000313|EMBL:ORY23845.1,
RC ECO:0000313|Proteomes:UP000193920};
RG DOE Joint Genome Institute;
RA Haitjema C.H., Gilmore S.P., Henske J.K., Solomon K.V., De Groot R.,
RA Kuo A., Mondo S.J., Salamov A.A., Labutti K., Zhao Z., Chiniquy J.,
RA Barry K., Brewer H.M., Purvine S.O., Wright A.T., Boxma B., Van Alen T.,
RA Hackstein J.H., Baker S.E., Grigoriev I.V., O'Malley M.A.;
RT "A Parts List for Fungal Cellulosomes Revealed by Comparative Genomics.";
RL Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10060,
CC ECO:0000256|RuleBase:RU361166}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ORY23845.1}.
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DR EMBL; MCOG01000229; ORY23845.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y2APG8; -.
DR STRING; 1754190.A0A1Y2APG8; -.
DR OrthoDB; 121538at2759; -.
DR Proteomes; UP000193920; Unassembled WGS sequence.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 3.90.1220.10; Cellulose docking domain, dockering; 3.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR002883; CBM10/Dockerin_dom.
DR InterPro; IPR009034; Dockerin_dom_fun_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR Pfam; PF02013; CBM_10; 3.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF64571; Cellulose docking domain, dockering; 3.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS51763; CBM10; 3.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10060};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10060};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10060};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10060};
KW Reference proteome {ECO:0000313|Proteomes:UP000193920};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 20..780
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5011834509"
FT DOMAIN 21..57
FT /note="CBM10"
FT /evidence="ECO:0000259|PROSITE:PS51763"
FT DOMAIN 67..102
FT /note="CBM10"
FT /evidence="ECO:0000259|PROSITE:PS51763"
FT DOMAIN 128..165
FT /note="CBM10"
FT /evidence="ECO:0000259|PROSITE:PS51763"
FT ACT_SITE 602
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 611
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 780 AA; 88563 MW; 833FB059A7020C6A CRC64;
MKNLLLISII GLLTSIAFAE ECWSEKLGYK CCKYCNVEYE DEEGKWGLEN DEWCGIKKSS
CDVYYKECFA SPAYPCCSKC NVVYTDEEGE WGYEKGSWCG IKDTCKNDYN SYSNTSTKTT
SVSTSEPTCW SEKLGYDCCK HCTVSYTDES GEWGVEEDDV WCGILKTCKI DFDYAIDMER
PYNSIPNPEK LHSRDYARIL NLNLRFYECQ WSGKAPEHKQ VYWRYDSALE DGADVGLDLT
GGFYDAGDHV KFNLPMSYAM TMLAWGGIDL YDNYVAAGQI DYLLQMVNHG ADYFVRCHPE
KEVIYAQVGV GDVDHCFWVP PELMTMERPT VCKLTPENGA SDVTGQMAAA LASASILNKK
YGNTEKAELY LKHAIEIFDF ADKYRDVYNN PCPEGASFYP SASYKDELVW GAAWVYRASG
DEKYYQKADE YYDSLALSGR NLIGPITWDD ASSGIVFLMS KPGFGSNWER YLGEAQHYVD
QYLNDKGART DGGMLWYSSV SGWGSARHAM AGTFLYCIFN TYHKPTDPKY FNFIQEQADY
ILGNNPFNRS LVVGSIPGYE PLNVHHRGSH GGYSPLSPPE HNMYPLYGAL AGGPLQNDFW
DDDRNNYVTN EVACDYNAGI TGVMAYLTPW TVSDNFDIDD SEPEADAHLI IAIEKTWEDV
YRLVANISTN LPTPAWELEF QMPIGHVFDD YSPIDAELVS WDEETRVVRL KNTPWNGPLT
ANLTETFRYQ GHGMKEEEGV ILPSHARLYY PKNKIYKNMK LTLYDKDGTT ILETKDRDSD
//
