ID A0A1Y2APK8_9FUNG Unreviewed; 372 AA.
AC A0A1Y2APK8;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 13-SEP-2023, entry version 21.
DE SubName: Full=Radical SAM enzyme {ECO:0000313|EMBL:ORY24503.1};
GN ORFNames=LY90DRAFT_706536 {ECO:0000313|EMBL:ORY24503.1};
OS Neocallimastix californiae.
OC Eukaryota; Fungi; Fungi incertae sedis; Chytridiomycota;
OC Chytridiomycota incertae sedis; Neocallimastigomycetes; Neocallimastigales;
OC Neocallimastigaceae; Neocallimastix.
OX NCBI_TaxID=1754190 {ECO:0000313|EMBL:ORY24503.1, ECO:0000313|Proteomes:UP000193920};
RN [1] {ECO:0000313|EMBL:ORY24503.1, ECO:0000313|Proteomes:UP000193920}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=G1 {ECO:0000313|EMBL:ORY24503.1,
RC ECO:0000313|Proteomes:UP000193920};
RG DOE Joint Genome Institute;
RA Haitjema C.H., Gilmore S.P., Henske J.K., Solomon K.V., De Groot R.,
RA Kuo A., Mondo S.J., Salamov A.A., Labutti K., Zhao Z., Chiniquy J.,
RA Barry K., Brewer H.M., Purvine S.O., Wright A.T., Boxma B., Van Alen T.,
RA Hackstein J.H., Baker S.E., Grigoriev I.V., O'Malley M.A.;
RT "A Parts List for Fungal Cellulosomes Revealed by Comparative Genomics.";
RL Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the radical SAM superfamily. Anaerobic
CC sulfatase-maturating enzyme family. {ECO:0000256|ARBA:ARBA00023601}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ORY24503.1}.
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DR EMBL; MCOG01000221; ORY24503.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y2APK8; -.
DR STRING; 1754190.A0A1Y2APK8; -.
DR OrthoDB; 9838at2759; -.
DR Proteomes; UP000193920; Unassembled WGS sequence.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR023885; 4Fe4S-binding_SPASM_dom.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR023819; Pep-mod_rSAM_AF0577.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023867; Sulphatase_maturase_rSAM.
DR NCBIfam; TIGR04084; rSAM_AF0577; 1.
DR NCBIfam; TIGR04085; rSAM_more_4Fe4S; 1.
DR PANTHER; PTHR43273; ANAEROBIC SULFATASE-MATURATING ENZYME HOMOLOG ASLB-RELATED; 1.
DR PANTHER; PTHR43273:SF2; RADICAL SAM DOMAIN CONTAINING PROTEIN; 1.
DR Pfam; PF13353; Fer4_12; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SFLD; SFLDG01067; SPASM/twitch_domain_containing; 1.
DR SFLD; SFLDG01104; Uncharacterised_Radical_SAM_Su; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000193920};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..16
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 17..372
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5012282327"
FT DOMAIN 1..219
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 372 AA; 42448 MW; FA24B53B6B39AE29 CRC64;
MLFFLTLTQN CNLACTYCGS DGNADIENIL EEDQPKEIRY KLNNLEKLKN IKDDLIICFY
GGEPLLKPRV IKKVIEMHPN AKFCLQTNGT LLNRFNTKLT NQMDTILISV DGGPEVTNKS
RGEGTWERAI NNARYIRKNG YKNDLVARMT VSNVSNVYED VRYLIELDDG LFDHVHWQLD
TQWSTPCFAG YDNFLDWRDN NYNVGITKLI NWFEKEIVEN SRVLGIAPFL QVIHDMITGR
KSRLRCGSGL DSFNVDTGGE INPCPIAPET HELAMIQDEN FQTPESLRDS VKIAGLCTDC
EILDLCGGRC LYANQTMWWG EEGFKEVCVT VKHLMNELKR ILPNVKQAIA DGKITLEDLN
YPKYNNSVEV IP
//
