ID A0A1Y2DYG2_9PEZI Unreviewed; 696 AA.
AC A0A1Y2DYG2;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=tRNA(Ile)-lysidine synthetase {ECO:0000256|ARBA:ARBA00013267};
DE EC=6.3.4.19 {ECO:0000256|ARBA:ARBA00013267};
GN ORFNames=BCR38DRAFT_342566 {ECO:0000313|EMBL:ORY64249.1};
OS Pseudomassariella vexata.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Xylariomycetidae; Xylariales; Pseudomassariaceae; Pseudomassariella.
OX NCBI_TaxID=1141098 {ECO:0000313|EMBL:ORY64249.1, ECO:0000313|Proteomes:UP000193689};
RN [1] {ECO:0000313|EMBL:ORY64249.1, ECO:0000313|Proteomes:UP000193689}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 129021 {ECO:0000313|EMBL:ORY64249.1,
RC ECO:0000313|Proteomes:UP000193689};
RG DOE Joint Genome Institute;
RA Mondo S.J., Dannebaum R.O., Kuo R.C., Labutti K., Haridas S., Kuo A.,
RA Salamov A., Ahrendt S.R., Lipzen A., Sullivan W., Andreopoulos W.B.,
RA Clum A., Lindquist E., Daum C., Ramamoorthy G.K., Gryganskyi A., Culley D.,
RA Magnuson J.K., James T.Y., O'Malley M.A., Stajich J.E., Spatafora J.W.,
RA Visel A., Grigoriev I.V.;
RT "Pervasive Adenine N6-methylation of Active Genes in Fungi.";
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP +
CC diphosphate + H(+) + lysidine(34) in tRNA(Ile2);
CC Xref=Rhea:RHEA:43744, Rhea:RHEA-COMP:10625, Rhea:RHEA-COMP:10670,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:82748, ChEBI:CHEBI:83665,
CC ChEBI:CHEBI:456215; EC=6.3.4.19;
CC Evidence={ECO:0000256|ARBA:ARBA00000047};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ORY64249.1}.
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DR EMBL; MCFJ01000007; ORY64249.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y2DYG2; -.
DR STRING; 1141098.A0A1Y2DYG2; -.
DR InParanoid; A0A1Y2DYG2; -.
DR OrthoDB; 460053at2759; -.
DR Proteomes; UP000193689; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0016879; F:ligase activity, forming carbon-nitrogen bonds; IEA:InterPro.
DR GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR CDD; cd01992; PP-ATPase; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_01161; tRNA_Ile_lys_synt; 1.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR011063; TilS/TtcA_N.
DR InterPro; IPR012094; tRNA_Ile_lys_synt.
DR InterPro; IPR012795; tRNA_Ile_lys_synt_N.
DR NCBIfam; TIGR02432; lysidine_TilS_N; 1.
DR PANTHER; PTHR43033; TRNA(ILE)-LYSIDINE SYNTHASE-RELATED; 1.
DR PANTHER; PTHR43033:SF1; TRNA(ILE)-LYSIDINE SYNTHASE-RELATED; 1.
DR Pfam; PF01171; ATP_bind_3; 2.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Reference proteome {ECO:0000313|Proteomes:UP000193689}.
FT DOMAIN 43..193
FT /note="tRNA(Ile)-lysidine/2-thiocytidine synthase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01171"
FT DOMAIN 284..330
FT /note="tRNA(Ile)-lysidine/2-thiocytidine synthase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01171"
SQ SEQUENCE 696 AA; 80392 MW; 427B275D9EE2371A CRC64;
MATISPVVHS SARAIRLAEF LDALRATCKP RFPHARGSQH RPVVLAISGG VDSMALAYLC
SKVRRFDPDF KISDNPVQKF QALVVDHGLR EGSNQEAEAV CKALWQMEIL SEVLPINWNK
EIGLGTDPNL LPNLESVARR VRYRKLGTVC ANRKVASLML AHHQDDQYET VLMRLLNGHG
SRGLRGMRRA SGIPECEGVH GAYNSGWIDD QSRQIPYYNT KPTQRQRKYL RYDLRHSINM
LLQEDEPAQA EDPWAGEENY GAYSYLNRDV SKSFVVGLLP IEDGGVNIYR PLLEFSKDRL
VATCLENNVP WWEDHTNKDP TLTMRNAVRH LWKNFELPQA LQKPAILELR ERSECRAQAL
EAEANRLLQQ TILHDFQPQV GTLVVQFPEL KPPKSTKYRP TSERYQGWVS RKREVAGLLI
QRILALVSPE TQTAPLANLQ NVISRLFPSL GNSAAELVAT PKAFPIAGVH FMPIESTPQS
VAMSTHPRTW YLARLPYPKN RPLPTWRTPY WSLTEQIAKH RKRVHWKWST WLRWHFWDGR
YWVRLAHRLP YRVVVMPFLI EHAKSFRESL LPRERKRLNE ALKKYTPGKV RYTLPAIYCE
EYLDLDNNWR PIKALDTSKM RMLALPSLDI QVPELDKWLL YEMRYKRADR NTLQMAGTLA
RSPVRATRRV GRAVRSMKGR IRLTRTAGFR SRSSNR
//
