ID A0A1Y2EZK4_9BASI Unreviewed; 788 AA.
AC A0A1Y2EZK4;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=cysteine--tRNA ligase {ECO:0000256|ARBA:ARBA00012832};
DE EC=6.1.1.16 {ECO:0000256|ARBA:ARBA00012832};
DE AltName: Full=Cysteinyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00031499};
GN ORFNames=BCR35DRAFT_267332 {ECO:0000313|EMBL:ORY76907.1};
OS Leucosporidium creatinivorum.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina;
OC Microbotryomycetes; Leucosporidiales; Leucosporidium.
OX NCBI_TaxID=106004 {ECO:0000313|EMBL:ORY76907.1, ECO:0000313|Proteomes:UP000193467};
RN [1] {ECO:0000313|EMBL:ORY76907.1, ECO:0000313|Proteomes:UP000193467}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=62-1032 {ECO:0000313|EMBL:ORY76907.1,
RC ECO:0000313|Proteomes:UP000193467};
RG DOE Joint Genome Institute;
RA Mondo S.J., Dannebaum R.O., Kuo R.C., Labutti K., Haridas S., Kuo A.,
RA Salamov A., Ahrendt S.R., Lipzen A., Sullivan W., Andreopoulos W.B.,
RA Clum A., Lindquist E., Daum C., Ramamoorthy G.K., Gryganskyi A., Culley D.,
RA Magnuson J.K., James T.Y., O'Malley M.A., Stajich J.E., Spatafora J.W.,
RA Visel A., Grigoriev I.V.;
RT "Pervasive Adenine N6-methylation of Active Genes in Fungi.";
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ORY76907.1}.
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DR EMBL; MCGR01000033; ORY76907.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y2EZK4; -.
DR STRING; 106004.A0A1Y2EZK4; -.
DR InParanoid; A0A1Y2EZK4; -.
DR OrthoDB; 2140072at2759; -.
DR Proteomes; UP000193467; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004817; F:cysteine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006423; P:cysteinyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd00672; CysRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR HAMAP; MF_00041; Cys_tRNA_synth; 1.
DR InterPro; IPR015803; Cys-tRNA-ligase.
DR InterPro; IPR024909; Cys-tRNA/MSH_ligase.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR032678; tRNA-synt_1_cat_dom.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR NCBIfam; TIGR00435; cysS; 1.
DR PANTHER; PTHR10890:SF3; CYSTEINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR10890; CYSTEINYL-TRNA SYNTHETASE; 1.
DR Pfam; PF01406; tRNA-synt_1e; 1.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000313|EMBL:ORY76907.1}; ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000193467};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 35..487
FT /note="tRNA synthetases class I catalytic"
FT /evidence="ECO:0000259|Pfam:PF01406"
FT REGION 727..771
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 757..771
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 788 AA; 87548 MW; 8FE7CCC533D5486B CRC64;
MASTQDASRS KATQDSWILP TRTVEEPKLK VDFVTKKPGV VTWYNCGPTV YDASHMGHAR
NYMAQDIMRR ILRDYFGYEV HFVMNITDVD DKIILRARQS HLLDKYTADV TSSSTPLSPT
FLAEVQAAWS AYVFKTMKDS LAAPTSDDYA AAKLAWEEIL TSEKDLKWVE AQKAKEEKFG
MYLSAVRAGL VGLVEAEKAA KEGRIGVEEA KALITANHDA ISLWLDKKLG STVNDPTIFR
DLAAYWEDSY FKSMAALHVE RPTTLTRVSE YLPEIVTFVE GIVNRGLAYE AGGSVWFDTT
KFEGAKGEES KDGDDGWKHT YAKLQPWSKG NRELLEDGEG SLTSTTGKRS ASDFALWKAS
KPGEPEWPST WGPGRPGWHI ECSVMASAVL GEGMDVHSGG VDLAFPHHDN EIAQSEAFHN
CRQWVNYFLH TGHLHIEGLK MSKSLKNFVT IDDALLKYSA RQLRFSFLLQ TWNAKLDFKE
SAMQEVRAAE TLLNNFFAIV NALAAEAKAD SVASDGQHHF NQPELDLLDK LEQAQLAFRV
ALCDSFDTPK GLQIILDLVS ATNVYLARGR AEVNISSVVA VADWVTRMLR MFGLGEGAPT
NVRGERLIGW GTAAAPGQEG AGDRQEILMP YLRALSAFRD SVRKLAMEGA PASEILALSD
QFRDYDAVEL GIALDDQADG RALFKLMPPE SLRQARDAKL AAANEKAARK AATAAAAEVK
RLERLEKGRT PPTELFRSSP EYSAWDEQGI PTLDKEGVEV PKSRKKKCQK EWDAQKKLHD
EFLKESSK
//
