ID   A0A1Y2F7G6_PROLT        Unreviewed;       666 AA.
AC   A0A1Y2F7G6;
DT   30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT   30-AUG-2017, sequence version 1.
DT   27-MAR-2024, entry version 14.
DE   SubName: Full=Carotenoid oxygenase {ECO:0000313|EMBL:ORY79870.1};
GN   ORFNames=BCR37DRAFT_381303 {ECO:0000313|EMBL:ORY79870.1};
OS   Protomyces lactucae-debilis.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Taphrinomycetes;
OC   Taphrinales; Protomycetaceae; Protomyces.
OX   NCBI_TaxID=2754530 {ECO:0000313|EMBL:ORY79870.1, ECO:0000313|Proteomes:UP000193685};
RN   [1] {ECO:0000313|EMBL:ORY79870.1, ECO:0000313|Proteomes:UP000193685}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=12-1054 {ECO:0000313|EMBL:ORY79870.1,
RC   ECO:0000313|Proteomes:UP000193685};
RG   DOE Joint Genome Institute;
RA   Mondo S.J., Dannebaum R.O., Kuo R.C., Labutti K., Haridas S., Kuo A.,
RA   Salamov A., Ahrendt S.R., Lipzen A., Sullivan W., Andreopoulos W.B.,
RA   Clum A., Lindquist E., Daum C., Ramamoorthy G.K., Gryganskyi A., Culley D.,
RA   Magnuson J.K., James T.Y., O'Malley M.A., Stajich J.E., Spatafora J.W.,
RA   Visel A., Grigoriev I.V.;
RT   "Pervasive Adenine N6-methylation of Active Genes in Fungi.";
RL   Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC         Evidence={ECO:0000256|PIRSR:PIRSR604294-1};
CC       Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR604294-1};
CC   -!- SIMILARITY: Belongs to the carotenoid oxygenase family.
CC       {ECO:0000256|ARBA:ARBA00006787}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:ORY79870.1}.
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DR   EMBL; MCFI01000014; ORY79870.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1Y2F7G6; -.
DR   STRING; 56484.A0A1Y2F7G6; -.
DR   OMA; TRLHRWR; -.
DR   OrthoDB; 318119at2759; -.
DR   Proteomes; UP000193685; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:InterPro.
DR   InterPro; IPR004294; Carotenoid_Oase.
DR   PANTHER; PTHR10543; BETA-CAROTENE DIOXYGENASE; 1.
DR   PANTHER; PTHR10543:SF89; CAROTENOID 9,10(9',10')-CLEAVAGE DIOXYGENASE 1; 1.
DR   Pfam; PF03055; RPE65; 1.
PE   3: Inferred from homology;
KW   Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR604294-1};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR604294-1};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Reference proteome {ECO:0000313|Proteomes:UP000193685};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        633..652
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   REGION          17..38
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         229
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT   BINDING         283
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT   BINDING         346
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT   BINDING         570
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
SQ   SEQUENCE   666 AA;  73755 MW;  974AEB21E1FF15D1 CRC64;
     MEEFAVAGVV PAEALVDAAS DKEKRSPRKS TRSRHPYLSG NYWPQPRELH LVPCTVEGQI
     PACLAGGQFV RNGPNAALPP AEGENYHMFD GDGMIHGVYF QEQADGNVVP LYINKYVRTD
     IFLASSYWGL NLLPSIASLI DPLSTTVRIL QSIIRSGAIA FLSGSTRLST ANTSLVFHDR
     RLLATCESGP PMHVNAPELE TIDWHTFPDD AGRGLSRVQG SSSEWITGHP KVDPVSGELL
     MFGYDIFQLF NPHIRLSILD SQGVHKHFQK PVYLQSAKPK MMHDFAITQK HVLIMDLPLT
     MSPFNVATAK PMLFFDANLI SKFGVLQRDD PDQIVWFETE SCMIFHTVNA WDDYNEAGQL
     VAFCLVACRF KGAKLVYAAG AIEMPQTGED VVHLYYYRFD LTTHKISHEY ALCDFPIEYP
     SINTQKLSLR NQYAYGASMR EGSFDTALNG AQIDVIVKCD IQALIQEPPT GVKVATSNAP
     PKMKHVTTLL LPQGCYASEA TFVSRDPADK TLDEDDGYLL LHVYDETTQD EYGDAPDDIG
     SEVWVVCAKT LILPPLAKIK LPQRVPYGLH GLWVSGQQIA LQRDATFNTK TGTMQSTRAT
     RDQTSVSLIA SFYKWSAIWA GKSALHGVSN GRFAISMLLS AVLAVLLTYR ALTILLQAMK
     HLTCTV
//