ID A0A1Y2KBK9_9PROT Unreviewed; 466 AA.
AC A0A1Y2KBK9;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE SubName: Full=Putative nickel-dependent hydrogenase, large subunit {ECO:0000313|EMBL:OSM07308.1};
GN ORFNames=MAIT1_04470 {ECO:0000313|EMBL:OSM07308.1};
OS Magnetofaba australis IT-1.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Magnetococcales;
OC Magnetococcaceae; Magnetofaba.
OX NCBI_TaxID=1434232 {ECO:0000313|EMBL:OSM07308.1, ECO:0000313|Proteomes:UP000194003};
RN [1] {ECO:0000313|EMBL:OSM07308.1, ECO:0000313|Proteomes:UP000194003}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IT-1 {ECO:0000313|EMBL:OSM07308.1,
RC ECO:0000313|Proteomes:UP000194003};
RX PubMed=27801294; DOI=10.1186/s12864-016-3064-9;
RA Araujo A.C., Morillo V., Cypriano J., Teixeira L.C., Leao P., Lyra S.,
RA Almeida L.G., Bazylinski D.A., Vasconcellos A.T., Abreu F., Lins U.;
RT "Combined genomic and structural analyses of a cultured magnetotactic
RT bacterium reveals its niche adaptation to a dynamic environment.";
RL BMC Genomics 17:726-726(2016).
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OSM07308.1}.
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DR EMBL; LVJN01000014; OSM07308.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y2KBK9; -.
DR STRING; 1434232.MAIT1_04470; -.
DR Proteomes; UP000194003; Unassembled WGS sequence.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR Gene3D; 1.10.645.10; Cytochrome-c3 Hydrogenase, chain B; 1.
DR InterPro; IPR001501; Ni-dep_hyd_lsu.
DR InterPro; IPR029014; NiFe-Hase_large.
DR PANTHER; PTHR42958:SF4; HYDROGENASE EXPRESSION_FORMATION PROTEIN HUPK; 1.
DR PANTHER; PTHR42958; HYDROGENASE-2 LARGE CHAIN; 1.
DR Pfam; PF00374; NiFeSe_Hases; 2.
DR SUPFAM; SSF56762; HydB/Nqo4-like; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|PIRSR:PIRSR601501-1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR601501-1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601501-1};
KW Nickel {ECO:0000256|PIRSR:PIRSR601501-1};
KW Reference proteome {ECO:0000313|Proteomes:UP000194003}.
FT BINDING 24
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 43
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 46
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 46
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 411
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 460
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 463
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 466
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
SQ SEQUENCE 466 AA; 50982 MW; 57412D79F14AC682 CRC64;
MQIDVRNGAV ADAWVTSPLY RGYEQMLQGK NPFDALVYVP RICGICSVAQ SVASGEALAA
AMGVTPTPNG QRARNLILAV ENVADHLTHF SLFFMPDFAR DAYRDAPWFD AAVKRFKAVS
GESARHALPA RAQLLNLLGI LAGKWPHSLA IQPGGSTRPI EPREQGRMAA LLHDFRHYLE
TRLFGDSLET IANLDSVAAL QHWAAQEPFE QSDFRFFLHL CQALELDQLG RAQDQFLSYG
AYSDEESHLF APGVMRDGAI SPLESAQISE DHSHSWMAAA QTRQHPYEGV TRPAEDRQRG
YSWCKAPRLA QAPMEVGALA RQAMAQQPLM TELIAHSGGN VQNRVIARLL EVARVTLAME
QWVWALQPRA PYCAQAPAIE QGRGAGLIEA ARGALGHWIT VESGRIANYQ IIAPTTWNFS
PRDAGGGLGP LEQALIGAPV ASGEAEPVTV QHIVRSFDPC MVCTVH
//
