ID A0A1Y2LPU3_EPING Unreviewed; 785 AA.
AC A0A1Y2LPU3;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=B5807_09555 {ECO:0000313|EMBL:OSS45750.1};
OS Epicoccum nigrum (Soil fungus) (Epicoccum purpurascens).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Didymellaceae; Epicoccum.
OX NCBI_TaxID=105696 {ECO:0000313|EMBL:OSS45750.1, ECO:0000313|Proteomes:UP000193240};
RN [1] {ECO:0000313|EMBL:OSS45750.1, ECO:0000313|Proteomes:UP000193240}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ICMP 19927 {ECO:0000313|EMBL:OSS45750.1,
RC ECO:0000313|Proteomes:UP000193240};
RA Fokin M., Fleetwood D., Weir B.S., Villas-Boas S.G.;
RT "Genome sequence of the saprophytic ascomycete Epicoccum nigrum ICMP 19927
RT strain isolated from New Zealand.";
RL Genome Announc. 0:0-0(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
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DR EMBL; KZ107853; OSS45750.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y2LPU3; -.
DR STRING; 105696.A0A1Y2LPU3; -.
DR InParanoid; A0A1Y2LPU3; -.
DR OMA; KWHGASA; -.
DR OrthoDB; 49764at2759; -.
DR Proteomes; UP000193240; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR CDD; cd00141; NT_POLXc; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR Gene3D; 3.40.50.10190; BRCT domain; 1.
DR Gene3D; 1.10.150.110; DNA polymerase beta, N-terminal domain-like; 1.
DR Gene3D; 3.30.210.10; DNA polymerase, thumb domain; 1.
DR InterPro; IPR036420; BRCT_dom_sf.
DR InterPro; IPR002054; DNA-dir_DNA_pol_X.
DR InterPro; IPR010996; DNA_pol_b-like_N.
DR InterPro; IPR028207; DNA_pol_B_palm_palm.
DR InterPro; IPR018944; DNA_pol_lambd_fingers_domain.
DR InterPro; IPR027421; DNA_pol_lamdba_lyase_dom_sf.
DR InterPro; IPR037160; DNA_Pol_thumb_sf.
DR InterPro; IPR022312; DNA_pol_X.
DR InterPro; IPR002008; DNA_pol_X_beta-like.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR029398; PolB_thumb.
DR PANTHER; PTHR11276:SF43; DNA POLYMERASE IV; 1.
DR PANTHER; PTHR11276; DNA POLYMERASE TYPE-X FAMILY MEMBER; 1.
DR Pfam; PF14792; DNA_pol_B_palm; 1.
DR Pfam; PF14791; DNA_pol_B_thumb; 1.
DR Pfam; PF10391; DNA_pol_lambd_f; 1.
DR Pfam; PF14716; HHH_8; 1.
DR PRINTS; PR00869; DNAPOLX.
DR PRINTS; PR00870; DNAPOLXBETA.
DR SMART; SM00483; POLXc; 1.
DR SUPFAM; SSF47802; DNA polymerase beta, N-terminal domain-like; 1.
DR SUPFAM; SSF81301; Nucleotidyltransferase; 1.
DR SUPFAM; SSF81585; PsbU/PolX domain-like; 1.
PE 4: Predicted;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000193240};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 456..784
FT /note="DNA-directed DNA polymerase X"
FT /evidence="ECO:0000259|SMART:SM00483"
FT REGION 264..357
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 383..440
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 265..303
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 313..331
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 424..440
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 517
FT /note="Nucleophile; Schiff-base intermediate with DNA; for
FT 5'-dRP lyase activity"
FT /evidence="ECO:0000256|PIRSR:PIRSR622312-50"
SQ SEQUENCE 785 AA; 87403 MW; F7C2341720D742E8 CRC64;
MPNDKLVAKQ AYFEDQKLLD LSNDETSDSN EALDEIESAM AEGERVKLVR QNSNFLGPTP
KETKADFEAH AARRRAAKRD RNQSFVRSTT APEAELTQSF PVTKLRARQS SHIDNNASSK
IKRITSLPDM TGLDQTPFYQ RMGVVPRELK NGKNVKPADN IKLEPESRQL LRGKVVYFYP
NDDVSMVRRT RIHMVIRLGA AWVNKWRDDI THVFVDDASH TYTQLLRHLN KAGLPRKVVL
AKFEPYVPQC IHFGSLMDPS ATRYSVKGAP QQSQASQKPE VSTVVSQTSG ESQTSLQIKP
TRKQKDALSS RKTDSLPGES TSRQSAMDKA PENSTVERVE DSFAMPSSDS HNEPPEVEVH
DAEFGYELQQ AIRETKAIAH LPIDDDEDEG SIAPSMVDAD GTDSGTDGEP SEIPSKSRKK
NFANEKTTST GPTRKAFDQS GFQCMDPSTG GYSSQNPNAH TIKILEEMGR YHDQMQDQWR
TLAYRRGVTT LKKQPTKIST AKQAAALPFI GTRLAEKIEE IVLTSRLRKL DNTKDDPLAE
VLRLFLGVYG AGLVQANKWI QTGHRTLDDL QAKAKLTDSQ KIGIEHYADF NARIPRAEVA
AHGAFVKNAL LEIDPAFEAT VMGSYRRRAA NSGDIDMLIS RPGASLAQLR TVVFNVLVPT
LSKAGFLKVA LAASHSSDAS GTKWHGASCL PGSTVWRRID LLLVPEAEMG AALIYFTGND
IFNRSMRLLA RKKGMRLNQK GLYKDVKRGW RGEKLNEGTL VEGRSERRIF EVLGVPWREP
GERIC
//
