ID   A0A1Y2ME43_EPING        Unreviewed;       579 AA.
AC   A0A1Y2ME43;
DT   30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT   30-AUG-2017, sequence version 1.
DT   27-MAR-2024, entry version 21.
DE   RecName: Full=Nuclear fusion protein KAR5 {ECO:0008006|Google:ProtNLM};
GN   ORFNames=B5807_01137 {ECO:0000313|EMBL:OSS54059.1};
OS   Epicoccum nigrum (Soil fungus) (Epicoccum purpurascens).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Pleosporomycetidae; Pleosporales; Pleosporineae; Didymellaceae; Epicoccum.
OX   NCBI_TaxID=105696 {ECO:0000313|EMBL:OSS54059.1, ECO:0000313|Proteomes:UP000193240};
RN   [1] {ECO:0000313|EMBL:OSS54059.1, ECO:0000313|Proteomes:UP000193240}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ICMP 19927 {ECO:0000313|EMBL:OSS54059.1,
RC   ECO:0000313|Proteomes:UP000193240};
RA   Fokin M., Fleetwood D., Weir B.S., Villas-Boas S.G.;
RT   "Genome sequence of the saprophytic ascomycete Epicoccum nigrum ICMP 19927
RT   strain isolated from New Zealand.";
RL   Genome Announc. 0:0-0(2017).
CC   -!- FUNCTION: Required for nuclear membrane fusion during karyogamy.
CC       {ECO:0000256|ARBA:ARBA00003389, ECO:0000256|RuleBase:RU368082}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000256|RuleBase:RU368082}. Nucleus membrane
CC       {ECO:0000256|RuleBase:RU368082}.
CC   -!- SIMILARITY: Belongs to the KAR5 family. {ECO:0000256|ARBA:ARBA00010473,
CC       ECO:0000256|RuleBase:RU368082}.
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DR   EMBL; KZ107838; OSS54059.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1Y2ME43; -.
DR   STRING; 105696.A0A1Y2ME43; -.
DR   InParanoid; A0A1Y2ME43; -.
DR   OMA; ECKLLEH; -.
DR   OrthoDB; 1940841at2759; -.
DR   Proteomes; UP000193240; Unassembled WGS sequence.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000742; P:karyogamy involved in conjugation with cellular fusion; IEA:UniProtKB-UniRule.
DR   GO; GO:0048288; P:nuclear membrane fusion involved in karyogamy; IEA:UniProtKB-UniRule.
DR   InterPro; IPR007292; Nuclear_fusion_Kar5.
DR   PANTHER; PTHR28012; NUCLEAR FUSION PROTEIN KAR5; 1.
DR   PANTHER; PTHR28012:SF1; NUCLEAR FUSION PROTEIN KAR5; 1.
DR   Pfam; PF04163; Tht1; 1.
PE   3: Inferred from homology;
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Endoplasmic reticulum {ECO:0000256|RuleBase:RU368082};
KW   Karyogamy {ECO:0000256|ARBA:ARBA00022459, ECO:0000256|RuleBase:RU368082};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU368082};
KW   Nucleus {ECO:0000256|RuleBase:RU368082};
KW   Reference proteome {ECO:0000313|Proteomes:UP000193240};
KW   Signal {ECO:0000256|RuleBase:RU368082, ECO:0000256|SAM:SignalP};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW   ECO:0000256|RuleBase:RU368082};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|RuleBase:RU368082}.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           19..579
FT                   /note="Nuclear fusion protein KAR5"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5012982940"
FT   TRANSMEM        407..425
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU368082"
FT   TRANSMEM        432..454
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU368082"
FT   COILED          228..259
FT                   /evidence="ECO:0000256|SAM:Coils"
SQ   SEQUENCE   579 AA;  63832 MW;  3278077981495ACC CRC64;
     MKLAIAVVIG GLTPISAALF HHYSPPADGA TVPSLDALVQ YTTSRNHETI SKAVEFLALM
     ETASTCTRAA VSHLISECKN FEGGSGSDKS QLDAYLDTVK KDYAAKLAVC EWLGAQPLDE
     ATPPLHCDIL VPSSRACGKD HWWPRAPVNT NKLCYPEYND RQFKTCFESL RAKSQHWTSY
     SNAGQNVVVM CQASRDALER ENHLEIFKNL TQVMGDVSSN IQHTTEAYAS LLREQTQFAD
     ELREAQDQFK KDAAAAQEKA LSTVSELDDK FHGFMSSSIS QLTTALADGQ AAELVRIREN
     LQNFSQDMIV ENSHLVSALR TELQQHHDRA IISLELNHQA QVHSHMILSG FMSDITDAAN
     KINATSGESL TIIDNIENRL DSLSSKTENI IKGFAFFSGL ARLATSMVQG LIATIGAIFI
     FNLLYRFSRQ LAVHLVGACS AAYFLHVCGV YQLLDESHRS PSFLDHLSNL NTTQKGVVIV
     LSLWLATYPI SRISTAISEV FLRILSSYWI REYRNESGAG YLLSVEIPQY AKTAQRKIDA
     LEQGIATGHT RLSGQQHRIL GSPDDGTRAP GFSSKVCKA
//