ID   A0A1Y2RC90_9BACT        Unreviewed;       813 AA.
AC   A0A1Y2RC90;
DT   30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT   30-AUG-2017, sequence version 1.
DT   27-MAR-2024, entry version 19.
DE   SubName: Full=Penicillin acylase family protein {ECO:0000313|EMBL:OSZ77751.1};
GN   ORFNames=CAP36_15410 {ECO:0000313|EMBL:OSZ77751.1};
OS   Chitinophagaceae bacterium IBVUCB2.
OC   Bacteria; Bacteroidota; Chitinophagia; Chitinophagales; Chitinophagaceae.
OX   NCBI_TaxID=1985174 {ECO:0000313|EMBL:OSZ77751.1, ECO:0000313|Proteomes:UP000194400};
RN   [1] {ECO:0000313|EMBL:OSZ77751.1, ECO:0000313|Proteomes:UP000194400}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IBVUCB2 {ECO:0000313|EMBL:OSZ77751.1,
RC   ECO:0000313|Proteomes:UP000194400};
RA   Orr R.J.;
RT   "Draft genome sequences of novel bacteria, isolated from environmental
RT   samples.";
RL   Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|PIRSR:PIRSR001227-2};
CC       Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2};
CC   -!- SIMILARITY: Belongs to the peptidase S45 family.
CC       {ECO:0000256|ARBA:ARBA00006586}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OSZ77751.1}.
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DR   EMBL; NFUV01000002; OSZ77751.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1Y2RC90; -.
DR   STRING; 1985174.CAP36_15410; -.
DR   InParanoid; A0A1Y2RC90; -.
DR   OrthoDB; 9759796at2; -.
DR   Proteomes; UP000194400; Unassembled WGS sequence.
DR   GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR   CDD; cd03747; Ntn_PGA_like; 1.
DR   Gene3D; 1.10.1400.10; -; 1.
DR   Gene3D; 2.30.120.10; -; 1.
DR   Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR   Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1.
DR   InterPro; IPR029055; Ntn_hydrolases_N.
DR   InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR   InterPro; IPR043147; Penicillin_amidase_A-knob.
DR   InterPro; IPR023343; Penicillin_amidase_dom1.
DR   InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR   InterPro; IPR002692; S45.
DR   PANTHER; PTHR34218:SF4; ACYL-HOMOSERINE LACTONE ACYLASE QUIP; 1.
DR   PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1.
DR   Pfam; PF01804; Penicil_amidase; 1.
DR   PIRSF; PIRSF001227; Pen_acylase; 1.
DR   SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE   3: Inferred from homology;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001227-2};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000194400};
KW   Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT   ACT_SITE        282
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-1"
FT   BINDING         354
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT   BINDING         357
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
SQ   SEQUENCE   813 AA;  91529 MW;  C041CF5069060AF2 CRC64;
     MRIVPFAIST IVTAGLVFAL NKQWGTIPPV GKFVSPQHGF WQNAEPTGEG FNAELNFPEL
     KGKADVYFDE RLVPHVFAEN DEDLYFIQGF LHAKFRLFQM DLQTKAAAGR ASEIAGPKAI
     NFDKEQRRLG MVYAAENAMK EMDKDPATKI MFDAYTNGVN AYIKSLKEAE LPVEYKLLNF
     KPEEWTNFRT ALLLKMMAKM LSSGTENDLS NTNAKSIFIP DELKAMYPQV HDSLAPIVPK
     GTVFEKPGIV PVQPATADSL YFGNKEVIAA KEISKPDINN GSNNWVVAGS KTQSGVPILS
     NDPHLELSLP SIWYEMQLQT PTSNTYGVSL PGSPFVIIGF NDSIAWGVTN AQRDVKDYYN
     IKFKDNTKQE YWFNGKWEKS ARKVETIKVK GGADVLDTVA YTVFGPVMFD EAFSNDFSNK
     RNLAVRWAAH DPSNDGITFY KLNRAKNYDE YADAIKTFDC PGQNFVFASK TGDIAIWQQG
     KFPARWNRQG LYVMPGEDSS YMWQGFIPQA ENPHSKNPVE GFLQSANQRP VDSTYPYFIP
     GSYITPRGIT ISNKLTAMSG ITPDDMMKLQ NNYFNTLGED ARSILTGYVK ENELNATEKK
     YLDILRNWDL MATPDSKGQT IYQCWWDSLE IGIWKDELSR TTPISPFPDE QTTMELLKRD
     TALKFIDDIT TTKQETLFDV VTIALKKASV ELAKAEAEGK LEWTKFKNPT VYHLLKDALP
     AFARKGLPVG GNGNIVNAIT HSHGPSWRMV VQMSTPTEAY GVYPGGQSGN PGSQYYDDYL
     DNWVAGKYNK LWFMREGDRT DKNVKWTMKF SKG
//