ID A0A1Y2V3R4_9PEZI Unreviewed; 2158 AA.
AC A0A1Y2V3R4;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=E3 ubiquitin-protein ligase {ECO:0000256|RuleBase:RU366018};
DE EC=2.3.2.27 {ECO:0000256|RuleBase:RU366018};
GN ORFNames=M434DRAFT_396731 {ECO:0000313|EMBL:OTA92053.1};
OS Hypoxylon sp. CO27-5.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Xylariomycetidae; Xylariales; Hypoxylaceae; Hypoxylon.
OX NCBI_TaxID=1001938 {ECO:0000313|EMBL:OTA92053.1, ECO:0000313|Proteomes:UP000194361};
RN [1] {ECO:0000313|EMBL:OTA92053.1, ECO:0000313|Proteomes:UP000194361}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CO27-5 {ECO:0000313|EMBL:OTA92053.1,
RC ECO:0000313|Proteomes:UP000194361};
RX PubMed=28078400; DOI=10.1007/s00253-017-8091-1;
RA Wu W., Davis R.W., Tran-Gyamfi M.B., Kuo A., LaButti K., Mihaltcheva S.,
RA Hundley H., Chovatia M., Lindquist E., Barry K., Grigoriev I.V.,
RA Henrissat B., Gladden J.M.;
RT "Characterization of four endophytic fungi as potential consolidated
RT bioprocessing hosts for conversion of lignocellulose into advanced
RT biofuels.";
RL Appl. Microbiol. Biotechnol. 101:2603-2618(2017).
CC -!- FUNCTION: Ubiquitin ligase protein which is a component of the N-end
CC rule pathway. Recognizes and binds to proteins bearing specific N-
CC terminal residues that are destabilizing according to the N-end rule,
CC leading to their ubiquitination and subsequent degradation.
CC {ECO:0000256|RuleBase:RU366018}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900,
CC ECO:0000256|RuleBase:RU366018};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|RuleBase:RU366018}.
CC -!- SIMILARITY: Belongs to the UBR1 family. {ECO:0000256|ARBA:ARBA00009750,
CC ECO:0000256|RuleBase:RU366018}.
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DR EMBL; KZ112490; OTA92053.1; -; Genomic_DNA.
DR STRING; 1001938.A0A1Y2V3R4; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000194361; Unassembled WGS sequence.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniRule.
DR GO; GO:0071596; P:ubiquitin-dependent protein catabolic process via the N-end rule pathway; IEA:UniProtKB-UniRule.
DR CDD; cd16482; RING-H2_UBR1-like; 1.
DR CDD; cd19673; UBR-box_UBR3; 1.
DR Gene3D; 2.10.110.30; -; 1.
DR Gene3D; 1.10.10.2670; E3 ubiquitin-protein ligase; 1.
DR InterPro; IPR042065; E3_ELL-like.
DR InterPro; IPR044046; E3_ligase_UBR-like_C.
DR InterPro; IPR039164; UBR1-like.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR InterPro; IPR003126; Znf_UBR.
DR PANTHER; PTHR21497:SF24; E3 UBIQUITIN-PROTEIN LIGASE UBR1; 1.
DR PANTHER; PTHR21497; UBIQUITIN LIGASE E3 ALPHA-RELATED; 1.
DR Pfam; PF18995; PRT6_C; 1.
DR Pfam; PF02207; zf-UBR; 1.
DR SMART; SM00396; ZnF_UBR1; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS51157; ZF_UBR; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU366018};
KW Reference proteome {ECO:0000313|Proteomes:UP000194361};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU366018};
KW Ubl conjugation pathway {ECO:0000256|RuleBase:RU366018};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU366018};
KW Zinc-finger {ECO:0000256|RuleBase:RU366018}.
FT DOMAIN 83..155
FT /note="UBR-type"
FT /evidence="ECO:0000259|PROSITE:PS51157"
FT ZN_FING 83..155
FT /note="UBR-type"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00508"
FT REGION 401..441
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 471..512
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 404..420
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2158 AA; 244791 MW; EF8F277A3B5C09D8 CRC64;
MSTQEQQLCQ YLIELPKRYA FRYEEAACRD LLYNLFWSMA GGRPENLRLF FPEGRLTPKS
TLKLREAQGA VEGAEYTEAA RGKACGHIFR AGEASYSCKT CSTDETCCLC SKCFNATDHT
GHMVRISISP GNSGCCDCGD PEAWKIPMFC TIHSEREEDK SKGKGKEAAA LPEDVVSNIR
MTISRVLDFI CDVISCAPEQ LRQPKTKESI EQDEKMSRLA STYCGGDVEP PEEYALLLWN
DEKHTVKEVQ DQVARACSTT LDEGYNRAVE TDSIGRSILK YDKSIEKLLR CATVLEHIKI
TVTIRSSRDT FREQMCGTMI DWLNDIAGCV VGNDQNILRT VVCEELLKPW RKGSPGTHAM
PGKDGLEDEE KLEYDPLEAH SAALFIQRAR FLAETGNVAR LAELEDSDED DNDDEDEDMD
GGNRTPSSGE EDNDEDEDED EDVMMVDMRP EASADVSVSD WIAPGVASLE EEEATMAGYP
PPPPPPPVPR RTTRDRDLTP SDSDTAEPLI APTVYAKANL EIPKTPGLSK ADKAGPPKPG
RYWVETPAAY MERDSVHPFE DVFQRVRLDW LILFDLRMWK KVRNDLRSLY ISTVVTIPEF
KRVLGLRFAA LYTTLAQLYL IGDREPDHSI INISLQMLTT PSITAEVVER GNFLTTLMAI
LYTFLTTRQV GHPWEVSSSA VLGFDTGSVT NRRMYHFYVD LKFLLGSPHV QERMRTEERY
LMQFLDLVKL HQGICPNVRA VGEHVEYETD SWIGASLMTR EINRLCRLLS GSFRNLPEQD
SIYLSRAIRT TAKSVIINSI GAERARFTQA EIKDEVRFKS LSDFEFAGEA TRFEVVKFIV
EEQSISFHHA LHYTLSWLIE CGKNMSPQEL RSILSFTSQE LKMKPRSMGR KTMPRRDYSP
EDYLMAAFDY PLRVCTWLAQ IKAGMWVRNG ISLRHQAGTY KNILHRDVTH ARDIFLLQTA
MVVCNPSRVL ASIVDRFGME HWIKGLFEQT STGQDEIQHL DVVEDMVHFL IVLLSDRTSL
IPVKDEQQTH LMAMRRDITH VLCFRPLSFN EIASKLPEKF HEQEDFQRVL DEVSTFKPPE
GVSDVGTFEL KPEFIEDIDP YIAHYNKNQR EESEAAYRKW MAKKTGKPAE EIVYEPKLRP
IESGVFVGLA QFTSTGMFAQ IIYYCLLYPL VAAKLTPKVP FTRIETFLQV VLHLILIAIA
EDKADETELA AGASSFVYIA LTTHARSNFM QDAPNAKTIV SLLDMMSTKE EFKACHPKIA
LILKRMKQKY PQHFDTAYSR LGIPVDRIDT ASPASTHNAE EERERKKKAA LDRQAKVMAQ
FQQQQKSFLE MQGDIDWGEE EWDDMDEAKP VEEQKNFWKY PRGTCILCQE DTDDGRLYGT
FALFTESKIL RQTDLQDPDF VREAANTPIN LDRSAEAIRP FGLAHENREI VTKVNAEGQT
FLAERQGLGK GFPAKLCRTG PVSVGCGHIM HFSCFDMYME ATVRRHGHQI ARHHPESLER
LEFVCPLCKA LGNAFLPITW DGKEEAYPGI LQTDSDLSSF ISTYMTGRHF TAVVSRDQSQ
NAFANYLRTR MPECLLEKAS ASQSPYSIWD TGNFRPVSSV ASSSDTFSLA ATPGSSVRSH
SPPESQQGDK ELMLTYRRLR DTLRKNKLVA ATLDNDSRDD DLYASDTLAQ SVGFSISAAE
IQQRGVEAQY GMTLIERMPE QLLVQLRILS ETVSSYIAVG GLRHGGDNQI EREFCKASER
QYCQLFISQY FDTSIGDQRR PFDEYAPLLS QDVFIFLCET MFGLSVAHNM DPMHLVRLCY
LAEIVKVVHN VSRNLALSRW LEYMSNRNVE DPAMNNFANF FHYITVARMN ISQKLSHEAL
EPSSDLPNLG FEQPGFDTLE SLHSFVRKYA LVFLRKVTIL LHVHLGVDFN SHISPIPEAD
ELTRLTTALK LPSFDEMCTA LTPLSTQFGW PPRIEAIVKG WLRHQIRGHT ARHFAIEKGI
EQIPPPSAQI SHPGIFELVG LPKNYDTLIE ECAKRRCPKT GKDLADPIVC LMCGDIFCGQ
TMCCLMEYQE PGPDKPLLRI GGAQQHMLFK CQGNIGLFIN IRKCAIFYLH RRSGSFSNAP
YIDKYGEVDI GLRHGRQLFL NQKRYDSMLR NMWLSHGIPS FISRKLEADI NSGGWETI
//
