ID   A0A1Y2VHR9_9PEZI        Unreviewed;       615 AA.
AC   A0A1Y2VHR9;
DT   30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT   30-AUG-2017, sequence version 1.
DT   27-MAR-2024, entry version 20.
DE   RecName: Full=tyrosinase {ECO:0000256|ARBA:ARBA00011906};
DE            EC=1.14.18.1 {ECO:0000256|ARBA:ARBA00011906};
GN   ORFNames=M434DRAFT_388779 {ECO:0000313|EMBL:OTA96924.1};
OS   Hypoxylon sp. CO27-5.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Xylariomycetidae; Xylariales; Hypoxylaceae; Hypoxylon.
OX   NCBI_TaxID=1001938 {ECO:0000313|EMBL:OTA96924.1, ECO:0000313|Proteomes:UP000194361};
RN   [1] {ECO:0000313|EMBL:OTA96924.1, ECO:0000313|Proteomes:UP000194361}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CO27-5 {ECO:0000313|EMBL:OTA96924.1,
RC   ECO:0000313|Proteomes:UP000194361};
RX   PubMed=28078400; DOI=10.1007/s00253-017-8091-1;
RA   Wu W., Davis R.W., Tran-Gyamfi M.B., Kuo A., LaButti K., Mihaltcheva S.,
RA   Hundley H., Chovatia M., Lindquist E., Barry K., Grigoriev I.V.,
RA   Henrissat B., Gladden J.M.;
RT   "Characterization of four endophytic fungi as potential consolidated
RT   bioprocessing hosts for conversion of lignocellulose into advanced
RT   biofuels.";
RL   Appl. Microbiol. Biotechnol. 101:2603-2618(2017).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 L-dopa + O2 = 2 H2O + 2 L-dopaquinone; Xref=Rhea:RHEA:34287,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57504,
CC         ChEBI:CHEBI:57924; EC=1.14.18.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00000426};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-tyrosine + O2 = H2O + L-dopaquinone; Xref=Rhea:RHEA:18117,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57924,
CC         ChEBI:CHEBI:58315; EC=1.14.18.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00001038};
CC   -!- COFACTOR:
CC       Name=Cu(2+); Xref=ChEBI:CHEBI:29036;
CC         Evidence={ECO:0000256|ARBA:ARBA00001973};
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DR   EMBL; KZ112429; OTA96924.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1Y2VHR9; -.
DR   STRING; 1001938.A0A1Y2VHR9; -.
DR   Proteomes; UP000194361; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004503; F:tyrosinase activity; IEA:UniProtKB-EC.
DR   Gene3D; 2.60.310.20; -; 1.
DR   Gene3D; 1.10.1280.10; Di-copper center containing domain from catechol oxidase; 1.
DR   InterPro; IPR008922; Di-copper_centre_dom_sf.
DR   InterPro; IPR041640; Tyrosinase_C.
DR   InterPro; IPR002227; Tyrosinase_Cu-bd.
DR   PANTHER; PTHR11474:SF32; TYROSINASE; 1.
DR   PANTHER; PTHR11474; TYROSINASE FAMILY MEMBER; 1.
DR   Pfam; PF18132; Tyosinase_C; 1.
DR   Pfam; PF00264; Tyrosinase; 1.
DR   PRINTS; PR00092; TYROSINASE.
DR   SUPFAM; SSF48056; Di-copper centre-containing domain; 1.
DR   PROSITE; PS00497; TYROSINASE_1; 1.
DR   PROSITE; PS00498; TYROSINASE_2; 1.
PE   4: Predicted;
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Reference proteome {ECO:0000313|Proteomes:UP000194361};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           23..615
FT                   /note="tyrosinase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5012305411"
FT   DOMAIN          129..146
FT                   /note="Tyrosinase copper-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS00497"
FT   DOMAIN          334..345
FT                   /note="Tyrosinase copper-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS00498"
SQ   SEQUENCE   615 AA;  68939 MW;  872D4BEE4727394C CRC64;
     MSFARQVCFF ITSFFFFFTV VADFVQPGQY DYGFNVDLRL RKREPTQSHI VTGLPRVDGQ
     TQLRPDIRDL QKDEDKWNLY LLTLNWLQYT NQDSPFSWYQ ITGIHGAPAL TWGDVGPTPG
     NEHSGYCTHV SILFPTWHRP YLALYEQVLY NIAQFVASLY PPDLQDRFQK AASTFRIPYW
     DWAATPPDGV SVLPLAVGGS SSVNVSGPNG VQSISNPLFS YVFRPFNAST FPDFPYNTWH
     ETKRAPRPVN SPNATSNNSF VAHALDTHLP SFQQRLYNLF SNYPNYTTFS NEAWIPSGNN
     NDSYDSIESL HDAIHTVGGG VYGHLAIIAY SGFDPLFWLH HANVDRIFTM WQALYNDTYV
     VPQPAVYSSH TTSPGESEDS QTGLTPFFSN ETHFWTSDMA RDHEVFGYTY AEVANKSRAE
     VAASINRLYG NFSPISMLTR PTTNGLSWNG MTSAYPPVEA VFSGDKYREW IANIRVSKHA
     MGGSFSIHLF LEDVPSDPIS WPVASNLVGT MGVFAHKGVH GSKSHRMVAG TIPITSALMQ
     MVASGKIPSL HAEDIEPYLR LNLELRVSLV NGTEVDAHEV SGLHIEIASS MVKAPESESM
     LSEWGKVERH FDLFA
//