ID A0A1Y3P4F0_9PSED Unreviewed; 790 AA.
AC A0A1Y3P4F0;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE SubName: Full=Penicillin amidase {ECO:0000313|EMBL:OUM72433.1};
GN ORFNames=AUC60_17820 {ECO:0000313|EMBL:OUM72433.1};
OS Pseudomonas caspiana.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1451454 {ECO:0000313|EMBL:OUM72433.1, ECO:0000313|Proteomes:UP000195440};
RN [1] {ECO:0000313|EMBL:OUM72433.1, ECO:0000313|Proteomes:UP000195440}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FBF102 {ECO:0000313|EMBL:OUM72433.1,
RC ECO:0000313|Proteomes:UP000195440};
RX PubMed=28552245; DOI=10.1016/j.syapm.2017.04.002;
RA Busquets A., Gomila M., Beiki F., Mulet M., Rahimian H., Garcia-Valdes E.,
RA Lalucat J.;
RT "Pseudomonas caspiana sp. nov., a citrus pathogen in the Pseudomonas
RT syringae phylogenetic group.";
RL Syst. Appl. Microbiol. 40:266-273(2017).
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR001227-2};
CC Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2};
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000256|ARBA:ARBA00004418}.
CC -!- SIMILARITY: Belongs to the peptidase S45 family.
CC {ECO:0000256|ARBA:ARBA00006586}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OUM72433.1}.
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DR EMBL; LOHF01000016; OUM72433.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y3P4F0; -.
DR OrthoDB; 9760084at2; -.
DR Proteomes; UP000195440; Unassembled WGS sequence.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR CDD; cd03747; Ntn_PGA_like; 1.
DR Gene3D; 1.10.1400.10; -; 1.
DR Gene3D; 2.30.120.10; -; 1.
DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR InterPro; IPR043147; Penicillin_amidase_A-knob.
DR InterPro; IPR023343; Penicillin_amidase_dom1.
DR InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR InterPro; IPR002692; S45.
DR PANTHER; PTHR34218:SF5; PENICILLIN G ACYLASE; 1.
DR PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1.
DR Pfam; PF01804; Penicil_amidase; 1.
DR PIRSF; PIRSF001227; Pen_acylase; 1.
DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR001227-2};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2};
KW Periplasm {ECO:0000256|ARBA:ARBA00022764};
KW Signal {ECO:0000256|ARBA:ARBA00022729};
KW Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT ACT_SITE 244
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-1"
FT BINDING 184
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 316
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 319
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 452
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
SQ SEQUENCE 790 AA; 87484 MW; 788F001F544C7F75 CRC64;
MKNALRALTI LSLLIVLSAS LYVYSKQPTR EGTVGLSHLQ RSVAVNYDDR GVPHINAENE
TDLYRTLGYV HAQDRLFQME IMRRLARGEL AEVLGPKLLD TDKLFRSLRI RERASRYVEQ
MDHNSASWKA MEAYLDGVNQ YQDSHAKPME FDVLGIQKRP FTAEDTFSIA GYVAYSFAIA
FRTEPLLTYV RDELGSEYLK VFDLDWQPKG ALNLAASDWK DLGAIAQLSE QAMAGNGLPQ
FEGSNAWAIS GNRTKSGKPL LAGDPHISFS VPSVWYEAQL TAPGFELYGY HNALVPVAFL
GHNKDFGWSL TMFQNDDLDL IAEKVNPDNP NQVWYHDKWV DMTSSEQQIA VKGQDPVALT
LRTSPHGPII NDVLGESAGK TPIAMWWAFL DTENPIFDGF YQLNRADTLD KVRAAAAKVQ
APGLNIVWAN ANGDIGWWAA AQLPVRPTGV NPSFILDGST VQADKLGFYP FSTNPQEENP
ARGYVVSANA QPASPTGMPI PGYYNLADRG QQLNAQLSDN SVRWDLESSK ALQLGTTNAY
GPRLLAPLLP VLREVVKDPA ELRLVEQLAT WRGGYPVDST SATLFNQFLF NLTYNVFHPK
LGDAMFESLL NTKAVDAALP RLTASPDSPW WNGHRTETVK VAWDHSLLHL KATFGDDPSQ
WQWGKAHTLT HGHPLGSQKP LDKIVNVGPF AAPGTHEVTN NLSAQIDSAP WPVTYGPSTR
RLIDFADAAK ALTINPVGQS GVPFDKHYSD QAETYIKGGY EKAYLNEEDV ISNRKSTLIF
LPANAETPQK
//
