ID A0A1Y4CNY6_9ACTN Unreviewed; 624 AA.
AC A0A1Y4CNY6;
DT 30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT 30-AUG-2017, sequence version 1.
DT 13-SEP-2023, entry version 20.
DE RecName: Full=PTS system lactose-specific EIICB component {ECO:0000256|ARBA:ARBA00020834};
DE EC=2.7.1.207 {ECO:0000256|ARBA:ARBA00012802};
DE AltName: Full=EIICB-Lac {ECO:0000256|ARBA:ARBA00029639};
GN ORFNames=B5F79_01470 {ECO:0000313|EMBL:OUO48724.1};
OS Olsenella sp. An285.
OC Bacteria; Actinomycetota; Coriobacteriia; Coriobacteriales; Atopobiaceae;
OC Olsenella.
OX NCBI_TaxID=1965621 {ECO:0000313|EMBL:OUO48724.1, ECO:0000313|Proteomes:UP000196267};
RN [1] {ECO:0000313|Proteomes:UP000196267}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=An285 {ECO:0000313|Proteomes:UP000196267};
RA Medvecky M., Cejkova D., Polansky O., Karasova D., Kubasova T., Cizek A.,
RA Rychlik I.;
RT "Function of individual gut microbiota members based on whole genome
RT sequencing of pure cultures obtained from chicken caecum.";
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=lactose(out) + N(pros)-phospho-L-histidyl-[protein] = L-
CC histidyl-[protein] + lactose 6-phosphate(in); Xref=Rhea:RHEA:42400,
CC Rhea:RHEA-COMP:9745, Rhea:RHEA-COMP:9746, ChEBI:CHEBI:17716,
CC ChEBI:CHEBI:29979, ChEBI:CHEBI:64837, ChEBI:CHEBI:79080;
CC EC=2.7.1.207; Evidence={ECO:0000256|ARBA:ARBA00001568};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OUO48724.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; NFIZ01000001; OUO48724.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1Y4CNY6; -.
DR OrthoDB; 3169536at2; -.
DR Proteomes; UP000196267; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.2300; -; 1.
DR InterPro; IPR036095; PTS_EIIB-like_sf.
DR InterPro; IPR003501; PTS_EIIB_2/3.
DR InterPro; IPR013012; PTS_EIIB_3.
DR InterPro; IPR003352; PTS_EIIC.
DR InterPro; IPR004501; PTS_EIIC_3.
DR NCBIfam; TIGR00410; lacE; 1.
DR NCBIfam; TIGR00853; pts-lac; 1.
DR PANTHER; PTHR33989; -; 1.
DR PANTHER; PTHR33989:SF8; PERMEASE IIC COMPONENT; 1.
DR Pfam; PF02378; PTS_EIIC; 1.
DR Pfam; PF02302; PTS_IIB; 1.
DR SUPFAM; SSF52794; PTS system IIB component-like; 1.
DR PROSITE; PS51100; PTS_EIIB_TYPE_3; 1.
DR PROSITE; PS51105; PTS_EIIC_TYPE_3; 1.
PE 4: Predicted;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 102..126
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 146..163
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 183..200
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 230..247
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 290..311
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 346..366
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 386..414
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 7..416
FT /note="PTS EIIC type-3"
FT /evidence="ECO:0000259|PROSITE:PS51105"
FT DOMAIN 521..624
FT /note="PTS EIIB type-3"
FT /evidence="ECO:0000259|PROSITE:PS51100"
FT REGION 477..498
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 528
FT /note="Phosphocysteine; by EIIA"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00423"
SQ SEQUENCE 624 AA; 66815 MW; 0D308CD6A597C5F4 CRC64;
MEKLIALIEK GKPFFNAIAR NKYLKAIRDG FISVMPIIIV SSVFLLVSAV PNVWGFYWPA
EINDVLMKAY NYSMGIVGIC AAATTAKHFC DAQNRDLPKN NQINFISVMI ASLIGFLLLS
SDAISFTDNG VSISGLANGY MGSKGLLTAF ICAFATGIIY KFFIKRNITI RMPEQVPPNI
SQTFKDIIPF GVCLVFFWGF DFAFRNIFGF CFAEGVIQVF QPLFSAADGY VGLAIIYGAM
SLFWFVGVHG PSIIEPAISA ALISNLELNM AMYQAGEHAT AVLTKPLQDF VVAMGGTGAT
LVICFMFAFL AKSKEMRAVG RASAVPVCFG VNEPFLFGAP MVLNPIFFIP FIFAPIANIW
ITKFFIDVLG MNGFMFSLPW TTPAPVGIAM GLGLQPLAFL LIPALLIVDF LIYFPFFKVY
DREKCAEEAE ADQAEVAQRA AHKQAAMNAA FQGKADTASV ATGAVAKAAA SMAAEKKRAA
GQSMDEESAG NTTPAASDNA VASASSSTIA AASDPYAALN GRRVLVLCQG GGTSGLLANA
LAKAAREHGI DLETAADAYG SHLDIMPDFD LVILAPQAAS YFDDLKADGE RMGVACCVTR
GKQYIDLTRD GEASLAFVAQ QLGL
//
